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Now showing items 1 - 11 of 11

  • Role of miR-21 in alkalinity stress tolerance in tilapia

    Zhao, Yan   Wu, Jun-Wei   Wang, Yan   Zhao, Jin-Liang  

    MicrORNAs (miRNAs) are a class of short, evolutionary conserved non-coding RNA molecules, which are shown as the key regulators of many biological functions. External stress can alter miRNA expression levels, thereby changing the expression of mRNA target genes. Here, we show that miR-21 is involved in the regulation of alkalinity tolerance in Nile tilapia. Alkalinity stress results in a marked reduction in miR-21 levels. miR-21 loss of function could affect ion balance regulation, ROS production, and antioxidant enzyme activity in vivo. Moreover, miR-21 knockdown protects cell against alkalinity stress-induced injury in vitro. miR-21 directly regulates VEGFB and VEGFC expression by targeting the 3'-untranslated regions (UTRs) of their mRNAs, and inhibition of miR-21 significantly increases the levels of VEGFB and VEGFC expression in vivo. Taken together, our study reveals that miR-21 knockdown plays a protective role in alkalinity tolerance in tilapia. (C) 2016 Elsevier Inc. All rights reserved.
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  • The complete mitochondrial genome of Acleris fimbriana (Lepidoptera:Tortricidae)

    Zhao, Jin-Liang   Wu, Yu-Peng   Su, Tian-Juan   Jiang, Guo-Fang   Wu, Chun-Sheng  

    The yellow tortrix, Acleris fimbriana belongs to Tortricidae in Lepidoptera. We described the complete mitogenome of A. fimbriana, which is typical circular duplex molecules and 15,933 bp in length containing the standard metazoan set of 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and an A + T-rich region with macro-repeat sequences. All the inferred tRNA secondary structures show the common cloverleaf pattern, with the exception of trnS1(AGN) which lacks the DHU arm. The A. fimbriana mitochondrial genome has the same gene order with other lepidopterans.
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  • Molecular characterization and functional analysis of IRF3 in tilapia (Oreochrornis niloticus)

    Gu, Yi-Feng   Wei, Qun   Tang, Shou-Jie   Chen, Xiao-Wu   Zhao, Jin-Liang  

    Interferon regulatory factor 3 (IRF3) plays a key role in interferon (IFN) response and binding to the MN stimulatory response elements (ISREs) within the promoter of IFN and IFN-stimulated genes followed by virus infection. In the current study, we discovered one IRF3 homologue in tilapia genome and analyzed the characterizations and functions of tilapia IRF3. Tilapia IRF3 contains 1368 bp with an ORE of 455 aa. Structurally, tilapia IRF3 protein typically shares the conserved characterizations with other species' IRF3 homologues, displaying conserved DNA-binding domain, IRF association domain, serine-rich C terminal domain, and tryptophan residue cluster. Phylogenetic analysis illustrated that tilapia IRF3 belongs to the IRF3 subfamily. Real-time PCR revealed a broad expression pattern of tilapia IRF3 in various tissues. Subcellular localization analysis showed that tilapia IRF3 mainly resides in the cytoplasm, Western blot demonstrated that IRF3 was distributed in the cytoplasmic fraction. Functionally, IRF3 was found to be transcriptionally up-regulated by the poly I:C stimulation. Moreover, reporter assay elucidated that tilapia IRF3 serves as a regulator in mediating IFN response by increasing the activity of IFN-beta and ISRE-containing promoter. These data supported the view that tilapia IRF3 is a potential molecule in IFN immune defense system against viral infection. (C) 2015 Elsevier Ltd. All rights reserved.
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  • miR-429 regulation of osmotic stress transcription factor 1 (OSTF1) in tilapia during osmotic stress

    Yan, Biao   Zhao, Li-Hui   Guo, Jin-Tao   Zhao, Jin-Liang  

    The Nile tilapia represents an excellent model for osmoregulation study. Osmotic stress transcription factor 1 (OSTF1) identified in tilapia gill epithelium is a critical element of osmosensory signal transduction by means of transcriptional regulation. Thus, tight regulation of OSTF1 level is necessary for tilapia osmotic adaptation. microRNAs (miRNAs), have emerged as a crucial regulator of gene expression at post-transcriptional level. We reasoned that OSTF1 expression could be regulated by miRNAs. By bioinformatics analysis, we identified a putative miR-429 binding site in the OSTF1 mRNA. Interestingly, miR-429 is down-regulated in tilapia upon osmotic stress, consistent with OSTF1 protein up-regulation. miR-429 directly regulates OSTF1 expression by targeting its 3'-UTR, and inhibition of miR-429 substantially increases OSTF1 level in vivo. Moreover, miR-429 loss of function could influence the regulation of plasma osmolality and ion concentration responding to osmotic stress. Taken together, miR-429 is an endogenous regulator of OSTF1 expression, which participates in a regulatory circuit that allows rapid gene program transitions in response to osmotic stress. Crown Copyright (c) 2012 Published by Elsevier Inc. All rights reserved.
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  • Reduction in hysteresis losses and large magnetic entropy change in the B-doped La(Fe, Si)(13) compounds RID G-6874-2011

    Shen, Jun   Wang, Fang   Zhao, Jin-Liang   Wu, Jian-Feng   Gong, Mao-Qiong   Hu, Feng-Xia   Li, Yang-Xian   Sun, Ji-Rong   Shen, Bao-Gen  

    The effect of the B-addition on magnetic entropy change Delta S(M) and hysteresis loss in La(Fe, Si)(13) is studied. The maximal values of Delta S(M) for LaFe(11.9)Si(1.1), LaFe(11.5)B(0.4)Si(1.1), LaFe(11.5)Si(1.5), and LaFe(11.0)B(0.5)Si(1.5) are found to be 27.0, 26.1, 23.7, and 21.2 J/kg K at Curie temperature T(C) for a field change in 0-5 T, respectively. The maximal hysteresis losses around T(C) are 43 and 21 J/kg for LaFe(11.9)Si(1.1) and LaFe(11.5)Si(1.5), respectively, while almost no magnetic hysteresis is observed for the B-doped compounds. Our result reveals that a large Delta S(M) and a small hysteresis loss can be simultaneously achieved in NaZn(13)-type La(Fe, Si)(13) compounds by the addition of B. (C) 2010 American Institute of Physics. [doi: 10.1063/1.3349325]
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  • Copper amine oxidase-catalysed hydrogen peroxide involves production of nitric oxide in darkness-induced stomatal closure in broad bean

    Huang, Ai-Xia   Wang, Yong-Shun   She, Xiao-Ping   Mu, Juan   Zhao, Jin-Liang  

    Hydrogen peroxide is an important intermediate in darkness-induced stomatal closure. In the present work, we provide evidence that copper amine oxidase (CuAO) was involved in H2O2 production in darkness-induced stomatal closure in Vicia faba L. Darkness activated CuAO in intercellular washing fluid from leaves. Aminoguanidine (AG) and 2-bromoethylamine (BEA), which were both irreversible inhibitors of CuAO, significantly suppressed darkness-induced stomatal closure and H2O2 generation. The effects of AG and BEA were reversed only by H2O2 but not by other products of CuAO. These results indicate that CuAO participates in darkness-induced stomatal closure through its reaction product, H2O2. Furthermore, darkness-induced nitric oxide (NO) production and cytosolic alkalinisation were obviously inhibited by AG and BEA, and only H2O2, among the products of CuAO, could reverse the effects, implying that the CuAO-catalysed product H2O2 is required for NO production and cytosolic alkalinisation to a large extent in darkness-induced stomatal closure. In addition, butyric acid blocked but methylamine enhanced the ability of H2O2 to reverse the effect of BEA on NO production, suggesting that cytosolic alkalinisation is involved in CuAO-mediated NO generation in darkness-induced stomatal closure.
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  • Reduction of magnetic hysteresis loss in La(0.5)Pr(0.5)Fe(11.4)Si(1.6)H(x) hydrides with large magnetocaloric effects RID G-6874-2011

    Zhao, Jin-liang   Shen, Jun   Hu, Feng-xia   Li, Yang-xian   Sun, Ji-rong   Shen, Bao-gen  

    Magnetic properties and magnetocaloric effects (MCEs) have been investigated in hydrogenated La(0.5)Pr(0.5)Fe(11.4)Si(1.6)H(x) (x=0, 0.9, and 1.6) compounds. It is found that the Curie temperature T(C) can be tuned from 189 to 317 K by adjusting hydrogen content from 0 to 1.6. It is attractive that both thermal and magnetic hysteresis are remarkably reduced because of the weakness of the itinerant-electron metamagnetic transition after hydrogenation, while the large magnetic entropy change is retained. The maximal hysteresis loss at T(C) decreases from 17.8 to 2.3 J/kg as x increases from 0 to 1.6. For the samples with x=0, 0.9, and 1.6, the maximal values of -Delta S are 26.3, 24.1, and 22.1 J/kg K at T(C), with efficient refrigeration capacities of 463, 366, and 351 J/kg for a field change of 0-5 T, respectively. Large reversible MCE and small hysteresis with considerable value of refrigeration capacity indicate the potentiality of La(0.5)Pr(0.5)Fe(11.4)Si(1.6)H(x) hydrides as a candidate magnetic refrigerant around room temperature. (C) 2010 American Institute of Physics. [doi: 10.1063/1.3374635]
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  • Mitochondrial genome annotation and phylogenetic placement of Oreochromis andersonii and O. macrochir among the cichlids of southern Africa.

    Bbole, Ian   Zhao, Jin-Liang   Tang, Shou-Jie   Katongo, Cyprian  

    Genetic characterization of southern African cichlids has not received much attention. Here, we describe the mitogenome sequences and phylogenetic positioning of Oreochromis andersonii and O. macrochir among the African cichlids. The complete mitochondrial DNA sequences were determined for O. andersonii and O. macrochir, two important aquaculture and fisheries species endemic to southern Africa. The complete mitogenome sequence lengths were 16642 bp and 16644 bp for O. andersonii and O. macrochir respectively. The general structural organization follows that of other teleost species with 13 protein-coding genes, 2 rRNAs, 22 tRNAs and a non-coding control region. Phylogenetic placement of the two species among other African cichlids was performed using Maximum Likelihood (ML) and Bayesian Markov-Chain-Monte-Carlo (MCMC). The consensus trees confirmed the relative positions of the two cichlid species with O. andersonii being very closely related to O. mossambicus and O. macrochir showing a close relation to both species. Among the 13 mitochondrial DNA protein coding genes ND6 may have evolved more rapidly and COIII was the most conserved. There are signs that ND6 may have been subjected to positive selection in order for these cichlid lineages to diversify and adapt to new environments. More work is needed to characterize the southern Africa cichlids as they are important species for capture fisheries, aquaculture development and understanding biogeographic history of African cichlids. Bio-conservation of some endangered cichlids is also essential due to the threat by invasive species.=20
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  • Reduction of magnetic hysteresis loss in La[sub 0.5]Pr[sub 0.5]Fe[sub 11.4]Si[sub 1.6]H[sub x] hydrides with large magnetocaloric effects

    Zhao, Jin-liang   Shen, Jun   Hu, Feng-xia   Li, Yang-xian   Sun, Ji-rong   Shen, Bao-gen  

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  • Fatty Acid Composition,Lipogenic Enzyme Activities and Mrna Expression of Genes Involved in The Lipid Metabolism of Nile Tilapia Fed with Palm Oil

    Ayisi, Christian Larbi   Zhao, Jin-Liang  

    This study was aimed at elucidating the effects of replacing fish oil (FO) with palm oil (PO) on tissue fatty acid composition, lipogenic enzyme activities and mRNA expression of genes related to lipid metabolism in Nile tilapia, Oreochromis niloticus (6.72 +/- 0.14g). An eight week feeding trial was conducted using five isonitrogenous and isolipidic diets containing 0% PO, 25% PO, 50% PO, 75% PO and 100% PO. PO supplementation led to a significant increase in total saturated fatty acid (SFA), total mono unsaturated fatty acids (MUFA) and 18: 2n-6, whiles DHA, EPA, total n-3 as well as 20: 2n-6 were reduced significantly in the liver (P<0.05). With the exception of glycerol-3-phosphate acyltransferase (GPAT) enzyme activity, supplementing tilapia diet with PO significantly increased fatty acid synthesase (FAS), acetyl-CoA carboxylase (ACC), steroyl-CoA desaturase 1 (SCD1), ATP citrate lyase (ACYL), carnitine palmitoyltransferase Ia (CPTIa) and carnitine palmitoyltransferase Ib (CPT Ib) (P<0.05). In addition, significant/positive correlations were observed among dietary PO and/or liver tissue FA with FAS, ACC, SCD1 and ACYL mRNA expression while a negative correlation was recorded for CPTI mRNA expression. Generally, inclusion of PO in tilapia diets resulted in lipid accumulation in the liver and altered the key gene expression of lipid metabolism.
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  • Corn silk (Zea mays L.), a source of natural antioxidants with α-amylase, α-glucosidase, advanced glycation and diabetic nephropathy inhibitory activities

    Wang, Kai-Jin   Zhao, Jin-Liang  

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