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Now showing items 49 - 64 of 64

  • Na(+)/K(+)-ATPase-mediated signal transduction and Na(+)/K(+)-ATPase regulation

    Zhang, Linan   Zhang, Zhe   Guo, Huicai   Wang, Yongli  

    A number of studies suggest that Na(+)/K(+)-ATPase in caveolae interacts with neighboring membrane proteins and organizes cytosolic cascades of signaling proteins to send messages to intracellular organelles in different tissues, mostly in cardiac myocytes. Low concentration of ouabain binding to Na(+)/K(+)-ATPase activates Src/epidermal growth factor receptor complex to initiate multiple signal pathways, which include PLC/IP3/CICR, PI3K, reactive oxygen species (ROS), PLC/DG/PKC/Raf/MEK/ERK1/2, and Ras/Raf/MEK/ERK1/2 pathways. In cardiac myocytes, the resulting downstream events include the induction of some early response proto-oncogenes, activation of transcription factors, activator protein-1, and nuclear factor-kappa B, the regulation of a number of cardiac growth-related genes, and the stimulation of protein synthesis and myocyte hypertrophy and apoptosis. Conversely, several factors acting through signal pathways, such as protein kinases, Ca(2+), ROS, etc., can modulate the activity of the Na(+)/K(+)-ATPase.
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  • Combined liver-kidney perfusion enhances protective effects of normothermic perfusion on liver grafts from donation after cardiac death.

    He, Xiaoshun   Ji, Fei   Zhang, Zhiheng   Tang, Yunhua   Yang, Lu   Huang, Shanzhou   Li, Wenwen   Su, Qiao   Xiong, Wei   Zhu, Zebin   Wang, Linhe   Lv, Lei   Yao, Jiyou   Zhang, Linan   Zhang, Longjuan   Guo, Zhiyong  

    It has been shown that combined liver-kidney normothermic machine perfusion (NMP) is able to better maintain the circuit's biochemical milieu. Nevertheless, whether the combined perfusion is superior to liver perfusion alone in protecting livers from donation after circulatory death (DCD) is unclear. We aimed to test the hypothesis and explored the mechanisms. Livers from 15 DCD pig donors were subjected to either static cold storage (group A), liver-alone NMP (group B), or combined liver-kidney NMP (group C). Livers were preserved for 6 hours and reperfused ex vivo for 2 hours to simulate transplantation or were transplanted in situ. During perfusion, group C showed an improved acid-base and biochemical environment in the circuit over group B. After reperfusion, the architecture of the liver grafts was best preserved in group C, followed by group B, then group A, as shown by the histology and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling staining of both hepatocytes and biliary epithelium. Ki-67 staining showed substantial hepatocyte proliferation and biliary epithelial regeneration after perfusion in group B and group C. Group C produced more bile in the reperfusion phase than those in group A and group B, with more physiological bile composition and less severe biliary epithelium injury. Von Willebrand factor-positive endothelial cells and E-selectin expression decreased in both group B and group C. Combined liver-kidney NMP not only produced more adenosine triphosphate, protected the nitric oxide signaling pathway, but also diminished oxidative stress (high mobility group box-1 protein and 8-hydroxy-2-deoxy guanosine levels) and inflammatory cytokine (IL6 and IL8) release when compared with liver-alone NMP and CS. In addition, the 7-day survival rate of liver transplant recipients was higher in group C than that in groups A and B. In conclusion, combined liver-kidney NMP can better protect DCD livers from warm ischemia and reperfusion injury probably by maintaining the stability of the internal environment and by abolishing oxidative stress injury. Liver Transplantation 24 67-79 2018 AASLD. =C2=A9 2017 by the American Association for the Study of Liver Diseases.
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  • Comparative Transcriptome Analysis Reveals Candidate Genes Related to Structural and Storage Carbohydrate Biosynthesis in Kelp Saccharina japonica (Laminariales,Phaeophyceae)

    Zhang, Linan   Cao, Zengmei   Liang, Guangjin   Li, Xiaojie   Wu, Hao   Yang, Guanpin  

    Saccharina japonica is a brown macroalga that has been commercially cultivated in China for almost a century. As a natural raw material, it is widely used in the food and pharmaceutical industries, and it may potentially be useful for biofuel production. However, little is known about the genes involved in carbohydrate biosynthesis, and their regulation is less understood. In this study, the analysis of growth traits and alginate and mannitol contents suggested that sporophyte development could be divided into four stages. Accordingly, we performed transcriptome analysis of the S. japonica sporophyte. In total, 589 million clean reads were generated, and 4,514 novel genes were identified. Gene expression analysis revealed that 2,542 genes were differentially expressed. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis indicated that these genes were significantly enriched in "Carbon metabolism," "Photosynthesis," and "Photosynthesis-antenna proteins" pathways, which are important for metabolism of various carbohydrates during sporophyte development. Systematic analysis identified the genes encoding enzymes for the biosynthesis of cell wall carbohydrates (including alginate, fucoidan, and cellulose) and cytoplasm storage carbohydrates (mannitol, laminarin, and trehalose). Among them, some key genes associated with carbohydrate content were further identified based on detailed expression profiling, representing good candidates for further functional studies. This study provides a global view of the carbohydrate metabolism process and an important resource for functional genomics studies in S. japonica. The results obtained lay the basis for elucidating the molecular mechanism of carbohydrate biosynthesis and for genetic breeding of carbohydrates-related traits in kelp.
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  • Morphometric measurement of the patella on 3D model reconstructed from CT scan images for the southern Chinese population.

    Shang, Peng   Zhang, Linan   Hou, Zengtao   Bai, Xueling   Ye, Xin   Xu, Zhaobin   Huang, Xu  

    BACKGROUND: Due to racial differences in the morphology of the knee joint and due to most prostheses available in the market being designed using measurements from Caucasians, the objective of this study was to provide the morphometric data of the patella for the southern Chinese population for total knee arthroplasty (TKA), patellar resurfacing, and prostheses design.; METHODS: The CT slices of the knee joint were obtained from both knees of 40 Chinese volunteers (20 females, 20 males, and age from 20-25 years) by performing a computer tomographic scan. A 3D model was reconstructed by Mimics software based on the computed tomography images. Six metrical characteristics were measured by digital ruler. Statistical analysis was performed with the SPSS statistical program.; RESULTS: The mean, standard deviation and P values of measurements and ratios were calculated using SPSS. All dimensions showed a significant gender difference with P < 0.05, but the six variables of the left and right knees had no statistical significance with P > 0.05. In addition, we studied the relationship between six couples (H-W, H-T, H-HAF, W-T, W-HAF, T-HAF) of the four variables (H: height, W: width, T: thickness and HAF: height of articulating facet) that were measured, which showed a significant correlation.; CONCLUSIONS: Examination of the southern Chinese population revealed that males have larger patellae than women. In both genders, comparing data between left and right knees shows no statistically significant difference. Compared with Westerners in previous studies, the patella in our study was thin and small. There was a good linear regression correlation between measurements of the patella. The indirect measurement method on 3D models makes it easy to obtain anatomical data, and the results can provide a region and gender specific database for morphometric measurements of the patella, and can be helpful for designing implants suited for southern Chinese patients.=20
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  • CONSTRUCTION AND CHARACTERIZATION OF A TENTATIVE AMPLIFIED FRAGMENT LENGTH POLYMORPHISM-SIMPLE SEQUENCE REPEAT LINKAGE MAP OF LAMINARIA (LAMINARIALES, PHAEOPHYTA)

    Yang, Guanpin   Sun, Ying   Shi, Yuanyuan   Zhang, Linan   Guo, Shanshan   Li, Bingjun   Li, Xiaojie   Li, Zhiling   Cong, Yizhou   Zhao, Yushan   Wang, Wenquan  

    A tentative amplified fragment length polymorphism-simple sequence repeat (AFLP-SSR) linkage map of Laminaria was constructed using a haploid population of 40 gametophyte clones isolated from an individual of Dongfang No. 2, the first commercially cultured hybrid of a female gametophyte clone of L. japonica Aresch. [=Saccharina japonica (Aresch.) C. E. Lane, C. Mayes et G. W. Saunders] and a male one of L. longissima Miyabe [=Saccharina longissima (Miyabe) C. E. Lane, C. Mayes et G. W. Saunders]. To the map, 263 markers (255 AFLP, seven SSR, and the gametophyte sex) were assigned. The map consisted of 25 linkage groups (LGs) with >= four markers, five triplets, and 15 doublets, which is 1,629.0 centiMorgans (cM) in length, covering 66% of Laminaria genome. The maximum space between loci is 24.63 cM. A putative sex-determining region was identified in LG(2), which was characterized by a dense marker distribution around the gametophyte sex locus. The linkage map itself and the methodology associated with its construction will facilitate the genetic study and further trials of the linkage map construction of Laminaria.
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  • Development of genic SSR markers from an assembled Saccharina japonica genome

    Peng, Jie   Zhang, Linan   Li, Xiaojie   Cui, Cuiju   Wu, Ruina   Tian, Pingping   Li, Yan   Liu, Yanling  

    Saccharina japonica, a marine brown alga, is an economically important species that has been cultivated in China for approximately a century. We identified 11,973 simple sequence repeats (SSRs) in 58.3 Mb of predicted coding sequence from an assembled S. japonica genome using Illumina paired-end sequencing data. Trinucleotide SSRs were the most abundant motif. Twenty-six loci amplified by the 57 unique primer pairs we designed from trinucleotide genic SSRs (repeat number > 10) were polymorphic, among the 55 S. japonica individuals tested. The number of alleles per locus ranged from 2 to 7 (average 3.46). The observed and expected heterozygosity per locus ranged from 0.128 to 0.652 and from 0.130 to 0.676, respectively. Two loci deviated from the Hardy-Weinberg equilibrium and two others were in linkage disequilibrium. We demonstrate that genic SSRs can be efficiently identified from assembled and annotated genomes using Illumina sequencing data. The novel polymorphism markers that we have identified, with informative flanking sequences and unique positional relationships to the genome, should facilitate genetic diversity analysis and further genetic studies in this species.
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  • Research Progress on Ammonium Bisulfate Formation and Control in the Process of Selective Catalytic Reduction

    Zhou, Chaoyang   Zhang, Linan   Deng, Yue   Ma, Shuang-Chen  

    The formation mechanism of ammonium bisulfate (ABS) in the process of SCR flue gas denitrification and some research progresses on ABS are reviewed in the paper. The main factors affecting the formation of ABS are investigated, the formation temperature of ABS is in the range of about 190 degrees C-240 degrees C; ABS exists in vapor phase at temperature between 320 degrees C and 345 degrees C, and begins to decompose when the temperature is higher than 345 degrees C; the formation of the ABS can be reduced by reducing the amount of NH3 slip and SO3, in the flue gas. The adverse effects of ABS on catalyst and air preheater are discussed: ABS will cover the surface of catalyst and Bronsted acid sites, decreasing denitrification efficiency; it will deposit in the air preheater, fouling and corroding the air preheater and reduce its operational effectiveness; it also can reduce the resistivity of fly ash and the quality of the fly ash as well. The methods to control ABS formation and the measures to reduce its negative effects are proposed: Select appropriate operating temperature to reduce the ABS deposition over the SCR catalyst; replace the material of the air preheater components from steel to ceramic coating type and change the structure of the air preheater; Take proper methods to reduce NH3 slip and the oxidation rate of SO2, etc. (C) 2016 American Institute of Chemical Engineers Environ Prog, 35: 1664-1672, 2016
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  • The Regulation of GluN2A by Endogenous and Exogenous Regulators in the Central Nervous System

    Sun, Yongjun   Zhan, Liying   Cheng, Xiaokun   Zhang, Linan   Hu, Jie   Gao, Zibin  

    The NMDA receptor is the most widely studied ionotropic glutamate receptor, and it is central to many physiological and pathophysiological processes in the central nervous system. GluN2A is one of the two main types of GluN2 NMDA receptor subunits in the forebrain. The proper activity of GluN2A is important to brain function, as the abnormal regulation of GluN2A may induce some neuropsychiatric disorders. This review will examine the regulation of GluN2A by endogenous and exogenous regulators in the central nervous system.
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  • Force sensing of multiple-DOF cable-driven instruments for minimally invasive robotic surgery.

    He, Chao   Wang, Shuxin   Sang, Hongqiang   Li, Jinhua   Zhang, Linan  

    BACKGROUND: Force sensing for robotic surgery is limited by the size of the instrument, friction and sterilization requirements. This paper presents a force-sensing instrument to avoid these restrictions.; METHODS: Operating forces were calculated according to cable tension. Mathematical models of the force-sensing system were established. A force-sensing instrument was designed and fabricated. A signal collection and processing system was constructed.; RESULTS: The presented approach can avoid the constraints of space limits, sterilization requirements and friction introduced by the transmission parts behind the instrument wrist. Test results showed that the developed instrument has a 0.03N signal noise, a 0.05N drift, a 0.04N resolution and a maximum error of 0.4N. The validation experiment indicated that the operating and grasping forces can be effectively sensed.; CONCLUSIONS: The developed force-sensing system can be used in minimally invasive robotic surgery to construct a force-feedback system. Copyright =C2=A9 2013 John Wiley & Sons, Ltd.
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  • A study of morphological evolution of silicon microstructure based on phase field model

    Zhang, Linan   Zheng, Wei   Lee, Jungchul   Wu, Liqun   Kim, Dongchoul  

    A three dimensional model for a nano/microfabrications of silicon on nothing (SON) structure based on phase field model has been presented. The temperature induces morphological evolution of silicon substrate has been introduced and suggested as a great possibility for the evolution dynamics. Phase field model is employed to present the morphological evolution of silicon. The numerical simulations represent an efficient verification on the experimental work. The performed work could present a detailed process of the cavity formed in the silicon substrate under high temperature. The mechanism for making the cavity in the silicon substrate is the shape changes, which is caused by surface diffusion and driven by the minimization of their surface energy. The aim of this paper is providing a reliable fabrication process of silicon substrate in high temperature, and control the fabrication process when the annealing is performed. The performed simulation results are well consistent with the experimental works.
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  • Portable glucose meter:trends in techniques and its potential application in analysis

    Zhang, Linan   Gu, Chunchuan   Ma, Huan   Zhu, Langlang   Wen, Jiajun   Xu, Hanxiao   Liu, Hongying   Li, Lihua  

    A blood glucose meter is an electronic medical device used for determining the concentration of glucose in blood. These meters have undergone five phases of development: washed blood glucose meters, wiped blood glucose meters, colorimetric blood glucose meters, electrochemical blood glucose meters, and micro, multiple site blood glucose meters. Thanks to their speed, portability, low cost, and easy operation, blood glucose meters have been widely available for use in clinical diagnosis. Recently, coupling of target recognition elements (antibody-antigen recognition, nucleic acid hybridization, enzyme recognition, and click chemistry) with signal transduction and amplification strategies (glucose-generating enzymes, nicotinamide adenine dinucleotide (NADH)-generating enzymes, encapsulated glucose, nanomaterials, and cyclic amplification of DNA) has allowed various targets to be determined via the relationship between the signal of the blood glucose meter and the concentration of targets. In this paper, a brief review of the development and mechanism of blood glucose meters is given first. Then, more details on the application of blood glucose meters in analysis are described, including biomedical analysis, food analysis, and environmental analysis. Finally, the prospect of future development of blood glucose meters is also discussed
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  • Stability of aerobic granular sludge in a pilot scale sequencing batch reactor enhanced by granular particle size control.

    Long, Bei   Xuan, Xinpeng   Yang, Changzhu   Zhang, Linan   Cheng, Yuanyuan   Wang, Jianqi  

    Aerobic granulation was successfully achieved in a pilot scale sequencing batch reactor within 40 days. Then, stability of different particle size granules was explored according to their activity and resistance to ultrasonic crushing. Different particle size granules (0.3-0.6=E2=80=AFmm, 0.6-1=E2=80=AFmm, 1-1.43=E2=80=AFmm, 1.43-2=E2=80=AFmm, 2-3=E2=80=AFmm and 3-4=E2=80=AFmm) were exposed under different ultrasonic power separately. It was found that the granules with 2-3=E2=80=AFmm always had the maximum granulation rates after ultrasonic crushing. Meanwhile, activity data showed that the 2-3=E2=80=AFmm granules had the lowest specific oxygen utilization rates, which indicated that they were easier to maintain stability as the increase of their particle sizes was the slowest. So, 500=E2=80=AFmL mixed liquid of the reactor were taken out and sieved to obtain the 2-3=E2=80=AFmm granules, which were subsequently returned to the reactor to increase their proportion. Through the manual regulation, the proportion of 2-3=E2=80=AFmm granules kept increasing which gradually became dominant in the reactor. Under the strategy of 86 days of operation, the aerobic granules were regular and compact, which had good removal effects of the real wastewater. The results indicated that the stability of the system could be greatly enhanced by the method, which provided a new strategy to maintain the granular stability. Copyright =C2=A9 2019 Elsevier Ltd. All rights reserved.
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  • Astragalus Polysaccharide Protect against Cadmium-Induced Cytotoxicity through the MDA5/NF-kappa B Pathway in Chicken Peripheral Blood Lymphocytes

    Xie, Wanqiu   Ge, Ming   Li, Guangxing   Zhang, Linan   Tang, Zequn   Li, Ruyue   Zhang, Ruili  

    Cadmium (Cd) is a known environmental pollutant that is associated with inflammation, oxidative stress, and cell apoptosis. Astragalus polysaccharide (APS) is a major component of Astragalus membranaceus, a vital qi-reinforcing herb medicine with favorable immuneregulation properties. To study the effect of APS on the inhibition of the cadmium-induced injury of peripheral blood lymphocytes (PBLs) in chickens through the MDA5/NF-kappa B signaling pathway, PLBs acquired from 15-day-old chickens were divided into control group, Cd group, APS + Cd group, anti-MDA5 mAb + Cd group, BAY 11-7082 (a nuclear factor kappa-light chain-enhancer of activated B cells [NF-kappa B] inhibitor) + Cd group, APS group, anti-MDA5 mAb group, and BAY 11-7082 group. The transcription levels of melanoma differentiation-associated gene 5 (MDA5), interferon promoter-stimulating factor 1 (IPS-1), NF-kappa B, and inflammatory factors tumor necrosis factor (TNF)-alpha, interleukin (IL)-1 beta, and IL-6 were measured by quantitative real-time PCR. MDA5 protein expression was measured by western blotting. Levels of malondialdehyde (MDA), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) were measured by corresponding antioxidant kit. The morphological change of PBLs was measured by transmission electron microscopy. The results showed that Cd significantly increased the expression of MDA5, IPS-1, NF-kappa B, and their downstream cytokines, IL-1 beta and TNF-alpha, IL-6 in PLBs. In addition, a high level of MDA was observed in the Cd treatment group; the activities of GSH-Px and SOD were significantly lower in the Cd treatment group than those in controls (p < 0.05). Ultrastructural changes of PBLs showed that Cd promoted autophagy, apoptosis, and necrosis in PBLs. However, APS can efficiently improve Cd-induced cell damage by decreasing the activation of the MDA5 signaling pathway. The effect is consistent with that of anti-MDA5 mAb or/and BAY. The results indicated that APS inhibited Cd-induced cytotoxicity through the regulation of MDA5/NF-kappa B signaling.
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  • Stability and error estimates of BV solutions to the Abel inverse problem

    Zhang, Linan   Schaeffer, Hayden  

    Reconstructing images from ill-posed inverse problems often utilizes total variation regularization in order to recover discontinuities in the data while also removing noise and other artifacts. Total variation regularization has been successful in recovering images for (noisy) Abel transformed data, where object boundaries and data support will lead to sharp edges in the reconstructed image. In this work, we analyze the behavior of BV solutions to the Abel inverse problem, deriving a priori estimates on the recovery. In particular, we provide L-2-stability bounds on BV solutions to the Abel inverse problem. These bounds yield error estimates on images reconstructed from a proposed total variation regularized minimization problem.
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  • Nanotechnology applied to overcome tumor drug resistance.

    Gao, Zibin   Zhang, Linan   Sun, Yongjun  

    Emerging multidrug resistance (MDR) to chemotherapy is a major obstacle in successfully treating malignant diseases. Nanotechnology provides an innovative and promising alternative strategy compared to conventional small molecule chemotherapeutics to circumvent MDR. This review focuses on recent literature examples of nanotechnology applications to overcome MDR. The advantages and limitations of various nanotechnologies are discussed as well as possible approaches to overcome the limitations. Developing a practical nanotechnology-based drug delivery system requires further studies of the tumor microenvironment, the mechanisms of MDR to chemotherapy, the optimal dosage regimen of anticancer drugs and/or siRNA, the transport kinetics of nanocarriers in tumor stroma and the pharmacokinetics of drug-loaded nanocarriers within MDR tumor cells. Copyright =C2=A9 2012 Elsevier B.V. All rights reserved.
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  • Computational modeling of fabrication of nanoneedle based on multi-physics analysis

    Zhang, Linan   Guo, Ziwang   Wu, Liqun   Chen, Chao  

    A three-dimensional model for fabrication process of Ag2Ga nanoneedle based on phase field model has been performed. With the development of model, the fabrication of Ag2Ga nanoneedle which is induced by chemical reaction has been well presented. The model incorporates a number of components, such as silver and gallium, which plays a crucial role in the diffuse and chemical reaction process. A phase field model is employed to describe the fabrication process and then supply reliable information to control the morphological evolution of Ag2Ga nanoneedle. The purpose of this work is providing a reliable mechanism of fabrication process of Ag2Ga nanoneedle in room temperature, and control the structure of nanoneedle in different temporal perspectives.
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