MacDonald, Michael R.
Tay, Wan Ting
Teng, Tiew‐Hwa Katherine
Anand, Inder
Ling, Lieng Hsi
Yap, Jonathan
Tromp, Jasper
Wander, Gurpreet S.
Naik, Ajay
Ngarmukos, Tachapong
Siswanto, Bambang B.
Hung, Chung‐Lieh
Richards, A. Mark
Lam, Carolyn S. P.
Liew, Houng Bang
Narasimhan, Calambur
Park, Sang Weon
Reyes, Eugenio
Shimizu, Wataru
Zhang, Shu
Wu, Liqun
Ling, Tianyou
Li, Xinli
Zhang, Haifeng
Su, Yangang
Yu, Cheuk Man
Sastry, B K S
Gopi, Arun
Raghu, K
Sridevi, C
Kaur, Daljeet
Parikh, Keyur
Chandarana, Anish
Shah, Urmil
Chag, Milan
Baxi, Hemang
Gupta, Satya
Bhatia, Jyoti
Khakhkhar, Vaishali
Sankhla, Vineet
Patel, Tejas
Kapoor, Vipul
Tandon, Rohit
Chopra, Vijay
Kumar, Manoj
Sethi, Hatinder Jeet Singh
Verma, Rashmi
Sawhney, Jitendra
Sharma, Manish Kr.
Purayil, Mohanan Padinhare
Munawar, Muhammad
Pambudi, Jimmy Agung
Lukito, Antonia
Pardede, Ingrid
Thengker, Alvin
Damay, Vito
Danny, Siska Suridanda
Surarso, Rarsari
Yasuoka, Ryoubun
Asai, Kuniya
Ikeda, Takanori
Park, Sang‐Weon
Hong, Suk Keun
Lee, SookJin
Soo, Lim Dal
Kim, Dong‐Hyeok
Shim, Jaemin
Park, Seong‐Mi
Roh, Seung‐Young
Kim, Young Hoon
Kim, Mina
Choi, Jong‐Il
Na, Jin Oh
Rha, Seung Woon
Seo, Hong Seog
Oh, Dong Joo
Park, Chang Gyu
Kim, Eung Ju
Lee, Sunki
Joung, Boyoung
Uhm, Jae‐Sun
Lee, Moon Hyoung
Cho, In‐Jeong
Park, Hui‐Nam
Park, Hyung‐Wook
Cho, Jeong‐Gwan
Yoon, Namsik
Lee, KiHong
Kim, Kye Hun
Kim, Seong Hwan
Liew, Houng Bang
Saharudin, Sahrin
Beh, Boon Cong
Lee, Yu Wei
Yen, Chia How
Othman, Mohd Khairi
Augustine, Amie‐Anne
Asnawi, Mohd Hariz Mohd
Mojolou, Roberto Angelo
Tan, You Zhuan
Arbain, Aida Nurbaini
Wong, Chii Koh
Ghazi, Azmee Mohd
Khelae, Surinder Kaur
Chew, David S.P.
Yap, Lok Bin
Hussin, Azlan
Muhammad, Zulkeflee
Abidin, Imran Zainal
Zhudi, Ahmad Syadi Bin Mahmood
Sari, Nor Ashikin Md
Sridhar, Ganiga Srinivasaiah
Zuhdi, Ahmad Syadi Mahmood
Ismail, Muhammad Dzafir
Ong, Tiong Kiam
Cham, Yee Ling
Background/Aims: Doublecortin-like kinase 1 (DCLK1) is emerging as a tumor-specific stem cell marker in pancreatic cancer (PC). MicroRNA-195 (miR-195) plays an important role in many types of tumors. However, the roles of DCLK1 in cancer and miRNAs that directly regulate DCLK1 have not been elucidated. The goal of this study is to assess the effects of miR-195 on inhibiting DCLK1 and to clarify the regulating mechanism of miR-195-DCLK1 in PC cells. Methods: The expression of DCLK1 protein and miR-195 in PC tissues and adjacent healthy pancreatic tissues was detected by Western blot and quantitative reverse transcription polymerase chain reaction (qRT-PCR), respectively and the correlation between overall survival of PC patients and expression of DCLK1 was measured by Kaplan-Meier analysis. Bioinformatics tools were used to identify the target gene of miR-195. Effects of miR-195 and DCLK1 on proliferation and cell cycle of PC cells were analyzed by MTT, colony formation assays and flow cytometry. Transwell and wound-healing experiments were employed to examine the cellular migration and invasion. A xenograft mouse model was also used to test the effects of miR-195 on tumor growth and metastasis in vivo. Results: The expression level of DCLK1 and miR-195 shows an inverse correlation in PC tissues and cell lines. A higher DCLK1 level is associated with higher TNM (tumor, node, and metastasis) stage, higher rate of lymph node metastasis, and poor survival. Luciferase reporter assay shows that miR-195 directly targets DCLK1. Overexpression of miR-195 inhibits proliferation, migration and invasion of PC cells, whereas downregulation of miR-195 has an opposite role. These actions were similar to the effects of knockdown and overexpression of DCLK1, respectively. Conclusions: These data suggest that miR-195 has tumor suppressor roles in PC by targeting DCLK1. MiR-195-DCLK1 pathway may provide insight into PC progression and represent a novel, promising diagnostic and therapeutic target for PC. (C) 2017 The Author(s) Published by S. Karger AG, Basel
Rong, Hui
Jiao, Yangquan
Wu, Liqun
Gu, Yuan
Zhang, Liya
In this paper, we use a range of parameters to quantitatively analyze the heterogeneity of oolitic reservoirs aiming to establish a predictive relationship between the reservoir heterogeneity and seismic velocities. The Yudongzi Oolitic shoals on the Triassic carbonate platform margin in the Erlangmiao area, NW Sichuan Basin, contain two fades elements: a stabilized sand flat and a mobile fringe. The stabilized sand flat consists mainly of oolitic limestones with moderately sorted grains of 0.1-6 mm diameter that account for 40-65% of the rock components. The mobile fringe is characterized by residual oolitic dolostones in which the grains are extremely sorted with a diameter of 0.1-0.5 mm and account for 70-80% of the rock components. These oolitic shoals occurred in the lower part of the Feixianguan Formation in each of the four sequence sets (i.e., Pss1-Pss4), and they were continuously progradational from Pss1 to Pss4. Diagenesis and reservoir properties of the oolitic shoals are closely related to their depositional environments, with the mobile fringe tending to contain potential reservoirs. Predictive relationships between the geological properties and seismic velocities of the oolitic reservoirs show that the content, mean diameter, mean radius ratio, and standard deviation of grains and dolomite content are linearly related to seismic velocities, while the porosity has a logistical correlation with seismic velocities. The seismic velocities decrease either with an increase of grain content or dolomite content, or with a decrease of mean grain diameter, mean radius ratio or standard deviation, and they decrease rapidly at first, and then slowly with an increase of porosity. With these correlations, impedance data from seismic exploration could be used to predict depositional environments, mineral composition, and porosity distribution of the oolitic reservoirs. (C) 2012 Elsevier B.V. All rights reserved.
Background: Although the Barcelona Clinic Liver Cancer (BCLC) staging system suggests that patients with stage B hepatocellular carcinoma (HCC) should be treated with transcatheter arterial chemoembolization instead of surgical treatment, recent studies indicated that the prognosis of surgical resection for patients with BCLC stage B HCC was better than that of TACE. However, the portion of patients with stage B that will achieve better outcomes from surgical treatment remains unclear. In this study, we identified risk factors that influence the prognosis of BCLC stage B HCC after R0 surgical resection to determine whether some patients with stage B HCC may benefit more from R0 resection than other patients and to provide a guideline to estimate the tendency. Methods: The clinical data of 78 patients with BCLC stage B HCC after R0 surgical treatment within 11 years were analyzed retrospectively, using relapse or death as the endpoint. Kaplan-Meier survival and Cox regression analyses were used to study prognosis (disease-free survival, DFS and overall survival, OS) and independent risk factors. Results: For all stage B patients, 1-, 2-, and 5-year DFS rates were 62.5, 36.4, and 16.6 %, respectively. Cumulative tumor size > 5.0 cm and tumor number >= 4 were independent prognostic risk factors for DFS. The 1-, 2-, and 5-year DFS rates and OS rates of patients with at least one of these two factors were 49.0, 17.2, and 7.4 % (for DFS), and 78.6, 54.8, and 13.4 % (for OS), respectively, which were significantly lower than patients without these two factors (77.8, 58.3, and 27.2 % for DFS, and 94.4, 83.3, and 51.8 % for OS, respectively, P < 0.01). Conclusions: The analyses indicated that the outcomes of R0 resection were much better for patients with BCLC stage B HCC with two or three tumors and cumulative tumor sizes of <= 5.0 but > 3.0 cm than other patients with stage B.
Micro-dosing of fine cohesive powders is the key technology in additive manufacturing and especially in high-potency active pharmaceutical ingredients (HPAPI). However, high accuracy micro-dosing (<5 mg) of fine cohesive powder is less trivial and still remains a challenge because it is difficult to eliminate the aggregation phenomena caused by the strong interparticle cohesive forces (in small capillaries). This paper presents a novel micro-dose method of fine cohesive powders via a pulse inertia force system. A piezoelectric actuator is used to provide a high enough pulse inertia force for a tapered glass nozzle and drive powder particles in the nozzle to be discharged from the nozzle orifice with the help of particle self-gravity. The nozzles with outlet diameters in the range of 100-2000 mu m were fabricated via a glass heating process. The -lactose monohydrate powder is used as the micro-dosing powder. The influences of the tapered nozzle outlet diameter, amplitude of the applied pulse voltage, and angle of the nozzle axis on micro-dosing mass are researched. The minimum mean dose mass is 0.6 mg for a single pulse inertia force. The coefficient of variation of dose mass, which represents the micro-dosing stability, can be controlled below 5% when the dose mass is relatively small.
Rong, Hui
Jiao, Yangquan
Wu, Liqun
Gu, Yuan
Zhang, Liya
Li, Rong
Zeng, Fanping
The oolitic shoals of the Triassic carbonate platform margin in the Yudongzi (e +/- 1/4ae ' a) outcrop of Erlangmiao (a(0)OEe integral Za(0)(TM)) area in the northwestern Sichuan (a >> a center dot e) basin present a scarce opportunity to quantitatively describe their diagenesis and its effects on the acoustic velocity. Using a detailed field geologic survey, profiles illustration of typical depositional system, and systematic testing, five types of diagenesis have been identified in the oolitic shoals: micritization, cementation, compaction and pressolution, dissolution, and dolomitisation. The cementation is composed of four subtypes (micrite cements, fibrous calcite cements, granular calcite cements, and blocky calcite cements). The dissolution is formed from three subtypes (freshwater selective dissolution, burial non-selective dissolution, and burial selective dissolution). The dolomitisation is composed of three subtypes (fine-crystalline dolomites, microcrystalline dolomites, and medium-crystalline dolomites). In order to quantitatively describe the diagenetic fabric of oolitic shoals, the micritic grain content, calcite cement content, mean pore diameter, pore types, dolomite content, and dolomite types have been evaluated. Based on these data, the relationship between the acoustic velocity and diagenesis of oolitic shoals has been established. The results show that the diagenetic fabric is linearly related with the acoustic velocity, and the general trend observed is as expected a decrease of velocity as the micritic grain content, mean pore diameter and dolomite content increase, or the sparite cement content decreases. This study will demonstrate that the transformation of diagenetic facies will probably make the petrophysical properties of the oolitic shoals regularly changed. The reflection configuration of diagenetic facies in the oolitic shoals can be shown in the synthetic seismic model simulated according to the P-wave impedance and S-wave impedance.
Background and Aim: Roux-en-Y hepaticojejunostomy (RYHJ) is usually used to treat benign strictures of hilar bile ducts. However, RYHJ might also induce ascending cholangitis and recurrent hepatolithiasis. The present study aims to introduce a modified hepatic portal choledochoplasty with a pedicled graft of gallbladder (HPC) to treat this disease. Methods: One hundred and forty-nine patients, who had undergone HPC or RYHJ from January 1997 to January 2006 in our institutions, were included in this study, and the clinical data were retrospectively collected and analyzed. Results: The incidences of perioperative bile leakage and inflammatory ileus in patients treated with HPC were slightly lower than RYHJ without significant difference (1.89% vs 2.08% and 3.77% vs 5.21%, both P > 0.05). However, in a long-term follow up, patients treated with HPC had significantly lower incidences of cholangitis and recurrent hepatolithiasis (5.66% and 3.77%, respectively) than those treated with RYHJ (cholangitis, 21.88%; hepatolithiasis, 16.67%; both P < 0.05). Conclusion: Compared to RYHJ, HPC is a safer and more efficient method to treat benign strictures of hilar biliary ducts. It preserves the sphincter of Oddi and normal biliary duct pressure, thus avoiding bile reflux into the bile duct.
Magnesium alloy (AZ31) reinforced with carbon nanotubes (CNTs) and grapheme nanoplatelets (GNPs) were fabricated with the method of hot-pressing sintering and hot extrusion processes. GNPs and CNTs were predispersed with Al and Zn powders by ball milling used as precursor for sintering, which effectively guaranteed the integrity and dispersion of them. The microstructure and mechanical properties of the composites (denoted as Mg-3 wt% Al-1 wt% Zn-1 wt% (xCNTs + yGNPs)(x:y =3D 1:1, 1:2, 1:3) were investigated. The results show that the CNTs and GNPs are uniformly distributed in the matrix and closely combined with the matrix in nanoscale. Among the tested composites, Mg-3 wt% Al-1 wt% Zn-1 wt% (xCNTs + yGNPs)(x:y =3D 1:2) exhibits the most favorable mechanical properties, and the yield strength, tensile and compressed strength, and elongation of composites are substantially improved by the addition of 0.33 wt% CNTs and 0.67 wt% GNPs. Novel strengthening mechanisms such as three-dimensional reinforced structure formed by CNTs and GNPs are found for the remarkable improvement in mechanical properties.
Wu, Liqun
Jiao, Yangquan
Rong, Hui
Wang, Rui
Li, Rong
In Changxing (e center dot a...') stage, Manyue (ae >> aee)-Honghua (c(0)cent eS +/-) Section of Kaixian (a1/4EuroaZ), northeastern Sichuan (a >> a center dot e) Province was located in the platform-margin slope, which was the advantage area of reef-shoal depositional system developing. The strata of Changxing Formation are continuously exposed on both Honghua Section and Manyue Section. Four and two depositional cycles can be identified in Honghua Section and Manyue Section, respectively. They are all platform-margin reef-shoal deposits. Their lithologic associations have obvious sequence, which is as follows: micrite bioclast limestone, bafflestone, bindstone, framestone, and bioclast limestone, in order from below. The paleontological assemblages are controlled by water depth and genetic facies. Six paleontological assemblages can be identified in Honghua Section and Manyue Section; they are coral-calcareous algae-calcareous sponge assemblage, calcareous sponge-calcareous algae assemblage, calcareous sponge-calcareous algae-coral-bryozoan assemblage, calcareous sponge-calcareous algae-hydra assemblage, calcareous sponge-calcareous algae-hydra-bryozoan assemblage, and calcareous sponge-calcareous algae-hydra-bryozoan-coral assemblage. The study of lithologic associations and paleontological assemblages indicates that the water body shallowed upward in both the growth cycle of a single reef and the higher-grade depositional cycle. According to the water depth types of reef (bioherm) developing, three different reef-shoal depositional systems can be identified in study area: deep-water-type bioherm, transitional-type reef (bioherm), and more shallow-water-type reef.