Wu, Hongmei
Bock, Stefanie
Snitko, Mariya
Berger, Thilo
Weidner, Thomas
Holloway, Steven
Kanitz, Manuel
Diederich, Wibke E.
Steuber, Holger
Walter, Christof
Hofmann, Daniela
Wei?brich, Benedikt
Spannaus, Ralf
Acosta, Eliana G.
Bartenschlager, Ralf
Engels, Bernd
Schirmeister, Tanja
Bodem, Jochen
Dengue fever is a severe, widespread, and neglected disease with more than 2 million diagnosed infections per year. The dengue virus NS2B/NS3 protease (PR) represents a prime target for rational drug design. At the moment, there are no clinical PR inhibitors (PIs) available. We have identified diaryl (thio) ethers as candidates for a novel class of PIs. Here, we report the selective and noncompetitive inhibition of the serotype 2 and 3 dengue virus PR in vitro and in cells by benzothiazole derivatives exhibiting 50% inhibitory concentrations (IC(50)s) in the low-micromolar range. Inhibition of replication of DENV serotypes 1 to 3 was specific, since all substances influenced neither hepatitis C virus (HCV) nor HIV-1 replication. Molecular docking suggests binding at a specific allosteric binding site. In addition to the in vitro assays, a cell-based PR assay was developed to test these substances in a replication-independent way. The new compounds inhibited the DENV PR with IC(50)s in the low-micromolar or submicromolar range in cells. Furthermore, these novel PIs inhibit viral replication at submicromolar concentrations.
The present invention relates to the field of communications, and particularly, to a method and device for fixing resource leakage. The method for fixing resource leakage comprises: a resource providing device receiving a resource acknowledgment request message from a resource request device, the resource acknowledgment request message carrying a first piece of indication information, and the first piece of indication information being used for indicating resources that have been obtained by the resource request device from the resource providing device; the resource providing device searching, according to the first piece of indication information, for resources that have been allocated by the resource providing device to the resource request device but have not been obtained by the resource request device; the resource providing device initializing the found resources to an unallocated state. The present invention is advantageous in that leaked resources can obtain a chance of being re-allocated so as to fix resource leakage.
Wu, Hongmei
Taki, Faten A.
Zhang, Yanqiong
Dobbins, Dorothy L.
Pan, Xiaoping
To identify reliable reference genes for toxicological studies, 16 commonly-used reference genes were selected as candidates to evaluate their expression stabilities under experimental conditions in Caenorhabditis elegans. Sixteen candidates were composed of 12 protein-coding genes and 4 non-coding RNAs, they were act-2, ama-1, arp-6, cdc-42, csq-1, eif-3.C, idhg-1, mdh, pmp-3, rbd-1, tba-1, Y45F10D.4, 18S rRNA, Ce234, U18, and U6. Larval stage 1 synchronized hermaphrodites were exposed to benzo-alpha-pyrene (B alpha P), chlorpyrifos, diazinon, gossypol, zinc oxide nanoparticles, and the vehicle control DMSO for 30 h, respectively. Expression stabilities of candidate genes were analyzed using 4 independent evaluating approaches (BestKeeper, the delta Ct approach, geNorm, and NormFinder) followed by a comprehensive method. Results showed that there were slight differences in ranking order between evaluation methods due to their different assumptions and computations. The results also showed that responses of candidate genes to different chemicals were distinct, 18S rRNA was the best for B alpha P and chlorpyrifos, tba-1 was the most stable gene for diazinon and gossypol treatments, while pmp-3 was more stable for zinc oxide exposure. Additionally, results demonstrated that combinations of multiple genes were more reliable than individual gene, suggesting selecting two or more candidates as reference genes may generate more reliable results for toxicological studies.
Wu, Hongmei
Xia, Yang
Jiang, Jin
Du, Huanmin
Guo, Xiaoyan
Liu, Xing
Li, Chunlei
Huang, Guowei
Niu, Kaijun
Highlights • Aim was to access the effect of HMB on muscle loss in older adults. • HMB can prevent lean body mass loss in older adults. • HMB supplementation does not result in a significant change in fat mass. Abstract Background Beta-hydroxy-beta-methylbutyrate (HMB), a metabolite of the branched-chain amino acid leucine, has been investigated as a potential supplement to improve muscle quality; however, whether HMB supplementation has beneficial effects on muscle loss in older adults remains unclear. Design Systematic review with meta-analysis. Setting PubMed, Medline and EMBASE databases were searched from the earliest possible year to September 2014. Participants Individuals aged 65 years and older that reported absolute changes in body composition with use of HMB. Measurements Two review authors working independently reviewed the trials, and standard mean difference was calculated using a fixed effects model. Results A total of seven randomized controlled trials were included, in which 147 older adults received HMB intervention and 140 were assigned to control groups. The meta-analysis showed greater muscle mass gain in the intervention groups compared with the control groups (standard mean difference = 0.352 kg; 95% confidence interval: 0.11, 0.594; Z value = 2.85; P = 0.004). There were no significant fat mass changes between intervention and control groups (standard mean difference = −0.08 kg; 95% confidence interval: −0.32, 0.159; Z value = 0.66; P = 0.511). Conclusion Beta-hydroxy-beta-methylbutyrate supplementation contributed to preservation of muscle mass in older adults. HMB supplementation may be useful in the prevention of muscle atrophy induced by bed rest or other factors. Further studies are needed to determine the precise effects of HMB on muscle strength and physical function in older adults.
Wu, Hongmei
He, Cheng
Lin, Zhihua
Liu, Yang
Duan, Chunying
Metallohelical triangles consisting of chromophore units and hydrogen-bonding trigger sites were prepared by modulating two tridentate N(2)O units containing amide groups within a central benzene ring at the meta sites, for the selective detection of adenosine trisphosphate in aqueous media over other ribonucleotides.
Wu, Hongmei
Taki, Faten A
Zhang, Yanqiong
Dobbins, Dorothy L
Pan, Xiaoping
To identify reliable reference genes for toxicological studies, 16 commonly-used reference genes were selected as candidates to evaluate their expression stabilities under experimental conditions in Caenorhabditis elegans. Sixteen candidates were composed of 12 protein-coding genes and 4 non-coding RNAs, they were act-2, ama-1, arp-6, cdc-42, csq-1, eif-3.C, idhg-1, mdh, pmp-3, rbd-1, tba-1, Y45F10D.4, 18S rRNA, Ce234, U18, and U6. Larval stage 1 synchronized hermaphrodites were exposed to benzo-alpha-pyrene (BalphaP), chlorpyrifos, diazinon, gossypol, zinc oxide nanoparticles, and the vehicle control DMSO for 30h, respectively. Expression stabilities of candidate genes were analyzed using 4 independent evaluating approaches (BestKeeper, the delta Ct approach, geNorm, and NormFinder) followed by a comprehensive method. Results showed that there were slight differences in ranking order between evaluation methods due to their different assumptions and computations. The results also showed that responses of candidate genes to different chemicals were distinct, 18S rRNA was the best for BalphaP and chlorpyrifos, tba-1 was the most stable gene for diazinon and gossypol treatments, while pmp-3 was more stable for zinc oxide exposure. Additionally, results demonstrated that combinations of multiple genes were more reliable than individual gene, suggesting selecting two or more candidates as reference genes may generate more reliable results for toxicological studies. =20
Ordered nanoporous alumina thin films with interpenetrated structures were prepared via alternating voltage (AV) anodization of Al foils in oxalic acid aqueous solutions. Anodization at different time periods was systematically investigated to better understand the formation process of this unique structure. Meanwhile, the corresponding current-time transient curves were recorded to monitor the formation process. Based on the obtained results, the possible formation process of porous alumina thin films with interpenetrated structure was discussed. (C) 2018 Published by Elsevier B.V.
A white ear lobe chicken embryo (WELCE) fibroblast cell bank, containing 322 tubes of frozen cells, was successfully established from primary explants of 57 embryo samples. The cells were morphologically consistent with fibroblasts, and the growth curve was sigmoidal with a population doubling time (PDT) of 48 h. Karyotyping and G-banding indicated a total chromosome number of 2n 78; the rate of diploidy in the cell bank was 97.62%. The cells were also free from bacterial, fungal, viral and mycoplasma contamination. Analysis of lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) isoenzymes ruled out cross-contamination between cells. In order to study exogenous gene expression, six fluorescent proteins were transfected into the WELCE cells. The transfection efficiency of these genes was between 10.1 and 41.9%. The corresponding fluorescence was distributed throughout the cytoplasm and nucleus 24 h after transfection. The results indicate that the quality of the cell line meet the quality requirements of the ATCC (American Type Culture Collection). (c) 2008 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
Gu, Yeqing
Meng, Ge
Zhang, Qing
Liu, Li
Wu, Hongmei
Zhang, Shunming
Wang, Yawen
Zhang, Tingjing
Wang, Xuena
Cao, Xingqi
Li, Huiping
Liu, Yunyun
Li, Xiaoyue
Wang, Xiaohe
Sun, Shaomei
Zhou, Ming
Jia, Qiyu
Song, Kun
Niu, Kaijun
Purpose Thyroid hormones (THs) have multiple effects on lipid synthesis, mobilization, and degradation, suggesting that THs may affect the development of dyslipidemia. However, prospective studies on the association between serum THs levels and incident dyslipidemia in euthyroid subjects are limited. Therefore, we conducted a cohort study (similar to 5-year follow-up period, median: 3.0 years) to explore whether THs can affect incident dyslipidemia in a general euthyroid population aged 18 years old and over. Methods Dyslipidemia is characterized by elevated total cholesterol (TC), triglyceride (TG), or low-density lipoprotein cholesterol (LDL-C), or reduced high-density lipoprotein cholesterol (HDL-C). Serum free triiodothyronine (FT3), free thyroxine (FT4), and thyroid-stimulating hormone (TSH) were determined by chemiluminescence immunoassay. Multivariable Cox proportional hazards regression models were used to assess the association between baseline FT3, FT4, TSH, and the risk of various dyslipidemias. Results During follow-up period, the incidence of elevated TC, TG, LDL-C, and reduced HDL-C was 29.3%, 20.7%, 24.8%, and 19.5%, respectively. After adjustment for multiple confounders, we found that per unit increase in FT3 concentrations were associated with decreased incidence of elevated TC and LDL-C, and the hazard ratios (95% confidence interval) were 0.87 (0.79-0.97) (P < 0.01) and 0.897 (0.808-0.995) (P =3D 0.04), respectively. We also found a weak positive association between TSH and incidence of reduced HDL-C (P =3D 0.02). However, we found no association between FT4 and incident dyslipidemia. Conclusions Our results demonstrated that low FT3 was associated with high dyslipidemia risk, especially for elevated TC and LDL-C, and that TSH had a weak positive effect on incidence of reduced HDL-C.
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