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Now showing items 97 - 112 of 116

  • Discovery and Characterization of Potent Pan-Genotypic HCV NS5A Inhibitors Containing Novel Tricyclic Central Core Leading to Clinical Candidate

    Ramdas, Vidya   Talwar, Rashmi   Banerjee, Moloy   Joshi, Advait Arun   Das, Amit Kumar   Walke, Deepak Sahebrao   Borhade, Prashant   Dhayagude, Usha   Loriya, Rajesh   Gote, Ganesh   Bommakanti, Apparao   Sivaram, Aruna   Agarwal, Gautam   Goswami, Arnab   Nigade, Prashant   Mehta, Maneesh   Patil, Vinod   Modi, Dipak   Kumar, Hemant   Mallurwar, Sadanand   Dash, Amruta   Modi, Falguni   Kuldharan, Sandip   Srivastava, Pratima   Singh, Minakshi   Narasimham, Lakshmi   Gundu, Jayasagar   Sharma, Sharad   Kamboj, Rajender Kumar   Palle, Venkata P.  

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  • Matrix Metalloproteinase-8 Inhibition Prevents Disruption of Blood–Spinal Cord Barrier and Attenuates Inflammation in Rat Model of Spinal Cord Injury

    Kumar, Hemant   Jo, Min-Jae   Choi, Hyemin   Muttigi, Manjunatha S.   Shon, Seil   Kim, Byung-Joo   Lee, Soo-Hong   Han, In-Bo  

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  • Polymorphisms of miR-146a,miR-149,miR-196a2,and miR-499 are associated with osteoporotic vertebral compression fractures in Korean postmenopausal women

    Ahn, Tae-Keun   Kim, Jung-Oh   Kumar, Hemant   Choi, Hyemi   Jo, Min-Jae   Sohn, Seil   Ropper, Alexander E.   Kim, Nam-Keun   Han, In-Bo  

    Genetic factors have been shown to be a small but significant predictor for osteoporosis and osteoporotic fracture risk. We performed a case-control association study to determine the association between miR-146a, miR-149, miR-196a2, and miR-499 polymorphisms and osteoporotic vertebral compression fracture (OVCF) susceptibility. In total, 286 unrelated postmenopausal Korean women (57 with OVCFs, 55 with non-OVCFs, and 174 healthy controls) were recruited. All subjects underwent dual energy X-ray absorptiometry to determine BMD at the lumbar spine and femoral neck. We focused on four single nucleotide polymorphisms (SNPs) of pre-miRNA sequences including miR-146aC>G (rs2910164), miR-149T>C (rs2292832), miR-196a2T>C (rs11614913), and miR-499A>G (rs3746444). Genotype frequencies of these four SNPs were determined using polymerase chain reaction-restriction fragment length polymorphism analysis. The TT genotype of miR-149aT>C was less frequent in subjects with OVCFs, suggesting a protective effect against OVCF risk (Odds ratio [OR], 0.435; 95% confidence interval [CI], 0.22-0.85, p=3D0.014), whereas the miR-146aCG/ miR-196a2TC combined genotype was more frequent in OVCF patients (OR, 5.163; 95%CI, 1.057-25.21, p=3D0.043), suggesting an increase in OVCF risk. Additionally, combinations of miR-146a, -149, -196a2, and -449 showed a significant association with increased prevalence of OVCFs in postmenopausal women. In particular, the miR-146aG/-149T/-196a2C/-449G allele combination was significantly associated with an increased risk of OVCF (OR, 35.01; 95% CI, 1.919-638.6, p=3D0.001). Our findings suggest that the TT genotype of miR-149aT>C may contribute to decreased susceptibility to OVCF in Korean postmenopausal women. Conversely, the miR-146aCG/ miR-196a2TC combined genotype and the miR-146aG/-149T/-196a2C/-449G allele combination may contribute to increased susceptibility to OVCF. (c) 2017 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 36:244-253, 2018.
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  • Effect of pioglitazone and rosiglitazone on mediators of endothelial dysfunction, markers of angiogenesis and inflammatory cytokines in type-2 diabetes

    Vijay, Sudarshan K.   Mishra, Manish   Kumar, Hemant   Tripathi, K.  

    The purpose of this study was to assess the effects of PPAR-gamma agonists (pioglitazone and rosiglitazone) on mediators of endothelial dysfunction and markers of angiogenesis in patients with type-2 diabetes. Pioglitazone group showed favorable reductions in serum total cholesterol, triglycerides, LDL cholesterol, VLDL cholesterol and increase in HDL cholesterol as compared to rosiglitazone group, after 16 weeks of treatment and also with control group. There was significant reduction of CRP level in pioglitazone and rosiglitazone group. The level of serum TNF-alpha decreased significantly in pioglitazone and mildly decreased in rosiglitazone group. The level of VEGF, IL-8 and Angiogenin were increased in pioglitazone than rosiglitazone group. There were no significant changes observed in the serum angiogenin and IL-8 levels in the control group. Pioglitazone and rosiglitazone therapy in type-2 diabetes subjects have additional benefits of reducing mediators of endothelial dysfunction. Increase in angiogenesis markers in patients receiving pioglitazone could have variable effects in diabetic nephropathy and retinopathy as there may be increased vascular neogenesis. Pioglitazone has advantage over rosiglitazone in lowering lipid and proinflammatory cytokines.
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  • Elastic Deformations in 2D van der waals Heterostructures and their Impact on Optoelectronic Properties: Predictions from a Multiscale Computational Approach

    Kumar, Hemant   Er, Dequan   Dong, Liang   Li, Junwen   Shenoy, Vivek B.  

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  • Anti-inflammatory effect of Tauroursodeoxycholic acid in RAW 264.7 macrophages, Bone marrow-derived macrophages, BV2 microglial cells, and spinal cord injury.

    Kim, Seong Jun   Ko, Wan-Kyu   Jo, Min-Jae   Arai, Yoshie   Choi, Hyemin   Kumar, Hemant   Han, In-Bo   Sohn, Seil  

    This study aimed to investigate the anti-inflammatory effects of tauroursodeoxycholic acid (TUDCA) after spinal cord injury (SCI) in rats. We induced an inflammatory process in RAW 264.7 macrophages, BV2 microglial cells, and bone marrow-derived macrophages (BMM) using lipopolysaccharide (LPS). The anti-inflammatory effects of TUDCA on LPS-stimulated RAW 264.7 macrophages, BV2 microglial cells, and BMMs were analyzed using nitric oxide (NO) assays, quantitative real-time polymerase chain reactions (qRT-PCR), and enzyme-linked immunosorbent assays (ELISA). The pathological changes in lesions of the spinal cord tissue were evaluated by hematoxylin & eosin (H&E) staining, luxol fast blue/cresyl violet-staining and immunofluorescent staining. TUDCA decreased the LPS-stimulated inflammatory mediator, NO. It also suppressed pro-inflammatory cytokines of tumor necrosis factor-alpha (TNF-alpha), interleukin 1-beta (IL-1beta), cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS) in both mRNA and protein levels. In addition, TUDCA decreased prostaglandin E2 (PGE2). After SCI, TUDCA supported the recovery of the injury site and suppressed the expression of inflammatory cytokines such as iNOS, CD68 and CD86. In addition, TUDCA induced the expression of anti-inflammatory cytokine, Arg-1. In conclusion, TUDCA inhibits inflammatory responses in RAW 264.7 macrophages, BV2 microglial cells, and BMMs. TUDCA can be a potential alternative drug for SCI.=20
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  • In Situ Dealloying of Bulk Mg2Sn in Mg-Ion Half Cell as an Effective Route to Nanostructured Sn for High Performance Mg-Ion Battery Anodes

    Asl, Hooman Yaghoobnejad   Fu, Jintao   Kumar, Hemant   Welborn, Samuel S.   Shenoy, Vivek B.   Detsi, Eric  

    Nanostructured Sn as negative electrode material in Mg-ion batteries suffers from very slow magnesiation kinetics when its nanoscale feature sizes are not in the sub-100 nm range. Herein, we use electrochemical experiments in combination finite element modeling (FEM) to demonstrate a cost-effective route to nanostructured Sn for high performance Mg-ion battery anodes. Using FEM we found that antagonistic stresses developed during dealloying of Mg2Sn induce pulverization of the dealloyed material and formation of nanostructured Sn with the characteristic feature size in the sub-100 nm range. These results were further confirmed through electrochemical experiments using a Mg half cell consisting of bulk Mg2Sn particles with an average characteristic size larger than 10 mu m as the working electrode, cycled versus Mg metal as counter and reference electrodes, and all-phenyl complex (APC) electrolyte. Ex situ electron microscopy and diffraction techniques were used to study the working electrode material in the pristine, demagnesiated, and remagnesiated forms. The results suggest that the starting micrometer-sized Mg2Sn particles are converted into nanostructured beta-Sn with characteristic sizes ranging from 10 to 50 nm during the first demagnesiation. Electrochemical performance of the in situ formed nanostructured Sn was further investigated during subsequent (de)magnesiation cycles in combination with electrochemical impedance spectroscopy (EIS). EIS studies suggest the formation of passive films on the Mg2Sn electrode. A reversible capacity of 300 mAh/g was demonstrated over 150 cycles at the rate of C/S after application of a combined sequence of regular galvanostatic cycling with an oxidative pulse to control the passive film formation. This work is expected to open new avenues for cost-effective routes to high performance alloy type Mg-ion battery anodes without complex nanosynthesis steps.
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  • Isolation and characterization of a thermostable F\r 420\r :NADPH oxidoreductase from\r Thermobifida fusca

    Kumar, Hemant   Nguyen, Quoc-Thai   Binda, Claudia   Mattevi, Andrea   Fraaije, Marco W.  

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  • Electrical and ammonia gas sensing properties of poly (3,3'''-dialkylquaterthiophene) based organic thin film transistors fabricated by floating-film transfer method

    Kumar, Chandan   Rawat, Gopal   Kumar, Hemant   Kumar, Yogesh   Prakash, Rajiv   Jit, Satyabrata  

    Electrical and ammonia gas sensing properties of a poly (3,3''' - dialkylquaterthiophene) (PQT-12) based organic thin film transistor (OTFT) fabricated by floating-film transfer method (FTM) on a highly doped p-type silicon substrate has been presented possibly for the first time in this paper. The OTFT is also fabricated using conventional spin coating method for the comparison with our FTM coated devices. The field effect mobility, threshold voltage, on/off current ratio and sub-threshold swing have been found as similar to 8.77 x 10(-3) cm(2)/V-s, 13.9 V, 843 and 8.5 V/dec, respectively for FTM coated devices. The drain current measured for different ammonia gas concentrations varying from 10 ppm to 80 ppm is observed to be decreased with increased ammonia concentration. A gas response of similar to 56.4%, response time and recovery time of similar to 45 s and similar to 85 s, respectively at 80 ppm has been measured with a detection limit of 404 ppb for FTM coated devices. Significant changes in the values of field effect mobility, threshold voltage and on/off current ratio have been achieved at 80 ppm of ammonia gas with respect to their corresponding values measured prior to exposing the device to the ammonia gas. Both the electrical and ammonia gas sensing properties of FTM coated device are superior than spin coated devices. (C) 2017 Elsevier B.V. All rights reserved.
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  • LC-MS/MS Assay for Quantification of a Novel Antidiabetic Flavone Derivative S002-857 in Rat Plasma, Urine and Feces: Application in Pharmacokinetic Studies

    Gautam, Nagsen   Kushwaha, Hari Narayan   Kumar, Hemant   Pratap, Ram   Singh, Shio Kumar  

    This study aimed to develop an LC-MS/MS assay for the quantitation of S002-857 in plasma, urine and feces to evaluate the pharmacokinetic parameters and elimination pathway for further development of S002-857. A sensitive and selective LC-MS/MS method was developed and validated for estimating a novel antidiabetic flavone derivative, S002-857, synthesized by Central Drug Research Institute, CSIR, Lucknow, India. Plasma and urine samples were prepared by two-step liquid-liquid extraction with diethyl ether and feces by protein precipitation using acetonitrile. The analyte was chromatographed on a cyano column with methanol-ammonium acetate buffer (pH 4.6, 10 mM; 90:10, v/v). The calibration curves were linear over a range of 0.78-400 ng mL(-1) for plasma and 1.56-400 ng mL(-1) for urine and feces samples. The accuracy and precision were < 15% for plasma, urine and feces samples. The recoveries from spiked plasma, urine and feces samples were > 85%. S002-857 was stable in plasma for 8 h at ambient temperature, 30 days at -60 A degrees C and after three freeze-thaw cycles. The assay was successfully applied to determine the pharmacokinetic parameters and excretion profile in male rats.
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  • Short Communication on "Self-welding susceptibility of NiCr-B hardfaced coating with and without NiCr-B coating on 316LN stainless steel in flowing sodium at elevated temperature"

    Kumar, Hemant   Ramakrishnan, V.   Albert, S. K.   Bhaduri, A. K.   Ray, K. K.  

    The self-welding susceptibility between NiCr-B coated 316LN stainless steel and the base metal, and that between NiCr-B hardfaced coatings has been evaluated in flowing sodium at 823 K for 90 and 135 days under contact stress of 8.0 and 11.0 MPa using a fabricated set-up. Neither any self-welding could be observed nor could any damage be detected on the specimen surfaces of the selected materials under the imposed experimental conditions, which indicate their satisfactory potential for applications in Fast Breeder Reactors. (C) 2016 Elsevier B.V. All rights reserved.
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  • Short Communication on “Self-welding susceptibility of NiCr-B hardfaced coating with and without NiCr-B coating on 316LN stainless steel in flowing sodium at elevated temperature”

    Kumar, Hemant   Ramakrishnan, V.   Albert, S.K.   Bhaduri, A.K.   Ray, K.K.  

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  • Safety and tolerability of intradiscal implantation of combined autologous adipose-derived mesenchymal stem cells and hyaluronic acid in patients with chronic discogenic low back pain:1-year follow-up of a phase I study

    Kumar, Hemant   Ha, Doo-Hoe   Lee, Eun-Jong   Park, Jun Hee   Shim, Jeong Hyun   Ahn, Tae-Keun   Kim, Kyoung-Tae   Ropper, Alexander E.   Sohn, Seil   Kim, Chung-Hun   Thakor, Devang Kashyap   Lee, Soo-Hong   Han, In-Bo  

    Background: Adipose tissue-derived mesenchymal stem cells (AT-MSCs) offer potential as a therapeutic option for chronic discogenic low back pain (LBP) because of their immunomodulatory functions and capacity for cartilage differentiation. The goal of this study was to assess the safety and tolerability of a single intradiscal implantation of combined AT-MSCs and hyaluronic acid (HA) derivative in patients with chronic discogenic LBP. Methods: We performed a single-arm phase I clinical trial with a 12-month follow-up and enrolled 10 eligible chronic LBP patients. Chronic LBP had lasted for more than 3 months with a minimum intensity of 4/10 on a visual analogue scale (VAS) and disability level >=3D 30% on the Oswestry Disability Index (ODI). The 10 patients underwent a single intradiscal injection of combined HA derivative and AT-MSCs at a dose of 2 x 10(7) cells/disc (n=3D5) or 4x10(7) cells/disc (n=3D5). Safety and treatment outcomes were evaluated by assessing VAS, ODI, Short Form-36 (SF-36), and imaging (lumbar spine X-ray imaging and MRI) at regular intervals over 1 year. Results: No patients were lost at any point during the 1-year clinical study. We observed no procedure or stem cell-related adverse events or serious adverse events during the 1-year follow-up period. VAS, ODI, and SF-36 scores significantly improved in both groups receiving both low (cases 2, 4, and 5) and high (cases 7, 8, and 9) cell doses, and did not differ significantly between the two groups. Among six patients who achieved significant improvement in VAS, ODI, and SF-36, three patients (cases 4, 8, and 9) were determined to have increased water content based on an increased apparent diffusion coefficient on diffusion MRI. Conclusions: Combined implantation of AT-MSCs and HA derivative in chronic discogenic LBP is safe and tolerable. However, the efficacy of combined AT-MSCs and HA should be investigated in a randomized controlled trial in a larger population.
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  • [ASME ASME 2015 International Mechanical Engineering Congress and Exposition - Houston, Texas, USA (Friday 13 November 2015)] Volume 8A: Heat Transfer and Thermal Engineering - CFD Analysis of a Fluidized Bed Combustor Based on Co-Firing

    Kumar, Hemant   Mohapatra, S. K.   Singh, Ravi Inder  

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  • [IEEE 2014 6th IEEE Power India International Conference (PIICON) - Delhi, India (2014.12.5-2014.12.7)] 2014 6th IEEE Power India International Conference (PIICON) - Performance analysis of maximum power point tracking algorithms for grid connected PV system

    Kumar, Hemant   Sharma, A K  

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  • [IEEE 2014 International Conference on Information Communication and Embedded Systems (ICICES) - Chennai, India (2014.2.27-2014.2.28)] International Conference on Information Communication and Embedded Systems (ICICES2014) - TCP congestion control with delay minimization in MANET

    Kumar, Hemant   Singh, Piyush  

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