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Now showing items 49 - 64 of 302

  • Chromatographic assay of pharmaceutical compounds under column overloading

    Jie Zhu   Cora Coscolluella  

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  • High-level expression of recombinant human paraoxonase 1 Q in silkworm larvae (Bombyx mori)

    Jie Zhu   Yuguan Ze   Chi Zhang   Yuhui Zang   Haiqin Lu   Ping Chu   Manji Sun and Junchuan Qin  

    Human serum paraoxonase 1 (hPON1) belongs to a family of enzymes that catalyze the hydrolysis of a broad range of esters and lactones. Although the very first identification of hPON1 might have been as a calcium-dependent paraoxonase/arylesterase, PON1 is in fact a lactonase associated with high-density lipoprotein and strongly stimulated by apoA-I. PON1 hydrolyzes various organophosphates, including insecticides and nerve gases. PON1 also plays a key role in prevention of atherosclerosis. Mediation of cholesterol efflux from macrophage is a key in vivo function of PON1. In present study, the hPON1 Q gene was cloned into baculovirus transfer vector pVL1392 and expressed in silkworm expression system. The rhPON1 Q presented two bands with every near molecular weight of about 40 and 43 kDa according to sodium dodecyl sulphate-polyacrylamide gel electrophoresis and Western blotting analysis. The expression level was up to 1,256 mg/L in haemolymph, about 50 times as high as that from BmN cells (24.8 mg/L). After purified by two chromatography steps (DEAE-Sepharose and HiTrap Chelating HP), the purity of rhPON1 Q was up to 90%, and the enzymatic properties are similar to serum hPON1.
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  • Magnetostrictive material and preparation method thereof

    The present invention relates to a Fe—Ga—Al-based magnetostrictive thin-sheet material and a process for preparation thereof. The raw materials used for production of the thin-sheet material is composed of the components according to the general Formula, Fe100-x-y-zGaxAlyMz, wherein x=10-30, y=1-10, and z=0.1-5, and M is any one, or more elements selected from V, Cr, Zr, Sb, Sn, Ti, SiC.
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  • Method and system for fast rotation of run-length encoded images

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  • Method and system for dispatching vehicle

    A method and a system for dispatching vehicle are provided. The method for dispatching vehicle includes the following steps: A. obtaining the vehicle information, which includes vehicle numbers, vehicle states and relative positions; B. placing the vehicle icons corresponding to the vehicle numbers in the corresponding positions on the virtual line schedule map according to the vehicle states and the relative positions; C. displaying the virtual line schedule map refreshed via step B. The system for dispatching vehicle includes vehicle information obtaining unit, vehicle states judging and processing unit, displaying unit and dispatching unit. The system realize the visual vehicle dispatching method by utilizing the virtual line schedule map, so as to implement vehicle monitoring and dispatching.
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  • Process for making n-(diphenylmethyl)piperazines

    The compound of formula (8), in racemic or single enantiomeric form, is useful in making N-(diphenylmethyl)-piperazines such as cetirizine and levocetrizine.
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  • Lycium barbarum polysaccharides regulate phenotypic and functional maturation of murine dendritic cells

    Jie Zhu   Lu-Hang Zhao   Xiao-Ping Zhao    Zhi Chen  

    Lycium barbarum polysaccharides (LBPs) have been known to have a variety of immunomodulatory functions including activation of T cells, B cells and NK cells. Dendritic cells (DC) are potent antigen-presenting cells that play pivotal roles in the initiation of the primary immune response. However, little is known about the immunomodulatory effects of LBPs on murine bone marrow derived dendritic cells (BMDC). In the present study, the effects of LBPs on the phenotypic and functional maturation of murine BMDC were investigated in vitro. Compared to the BMDC that were only subjected to treatment with RPMI1640, the co-expression of I-A/I-E, CD11c and secretion of IL-12 p40 by BMDC stimulated with LBPs (100 μg/ml) were increased. In addition, the endocytosis of FITC-dextran by LBPs-treated BMDC (100 μg/ml) was impaired, whereas the activation of proliferation of allogenic lymphocytes by BMDC was enhanced. Our results strongly suggest that LBPs are capable of promoting both the phenotypic and functional maturation of murine BMDC in vitro.
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  • Lycium barbarum polysaccharides regulate phenotypic and functional maturation of murine dendritic cells

    Jie Zhu   Lu-Hang Zhao   Xiao-Ping Zhao   Zhi Chen  

    Abstract Lycium barbarum polysaccharides (LBPs) have been known to have a variety of immunomodulatory functions including activation of T cells, B cells and NK cells. Dendritic cells (DC) are potent antigen-presenting cells that play pivotal roles in the initiation of the primary immune response. However, little is known about the immunomodulatory effects of LBPs on murine bone marrow derived dendritic cells (BMDC). In the present study, the effects of LBPs on the phenotypic and functional maturation of murine BMDC were investigated in vitro. Compared to the BMDC that were only subjected to treatment with RPMI1640, the co-expression of I-A/I-E, CD11c and secretion of IL-12 p40 by BMDC stimulated with LBPs (100 μg/ml) were increased. In addition, the endocytosis of FITC-dextran by LBPs-treated BMDC (100 μg/ml) was impaired, whereas the activation of proliferation of allogenic lymphocytes by BMDC was enhanced. Our results strongly suggest that LBPs are capable of promoting both the phenotypic and functional maturation of murine BMDC in vitro.
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  • Apparatus, and associated method, for synchronizing databases connected by way of a radio air interface

    A synchronization process, and associated apparatus, for placing database copies in match with one another by way of communications effectuated upon an air interface of a radio communication system. Hash functions are performed at a mobile node and at a network part upon mobile and network copies of database information. Comparison of the hash information formed therefrom provides a preliminary indication of whether the databases are in match with one another. If a determination is made that the databases are out of match, the data is compared, and the data is placed in match with one another responsive to such additional comparisons.
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  • Apparatus, and associated method, for synchronizing databases connected by way of a radio air interface

    A synchronization process, and associated apparatus, for placing database copies in match with one another by way of communications effectuated upon an air interface of a radio communication system. Hash functions are performed at a mobile node and at a network part upon mobile and network copies of database information. Comparison of the hash information formed therefrom provides a preliminary indication of whether the databases are in match with one another. If a determination is made that the databases are out of match, the data is compared, and the data is placed in match with one another responsive to such additional comparisons.
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  • Shock layer thickness and optimum linear velocity in displacement chromatography

    Jie Zhu   Georges Guiochon  

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  • Ultrastructure and physiochemical properties of CF1F0-ATPase from wheat leaves with atomic force microscopy

    Jie Zhu   Lian-Hong Guo    Guo-Dong Wang  

    The structural preferences of 1,2-dioleoyl-sn-glycerol glycolipids with glucose, galactose, maltose, and cellobiose as sugar head group were investigated under near physiological conditions with Fourier-transform infrared spectroscopy (FT-IR) and synchrotron radiation small-angle X-ray scattering (SAXS). Whereas all glycolipids have a very high fluidity at temperatures above 0 °C, the mono- and disaccharide compounds differ considerably in their aggregate structures. The monosaccharide compounds adopt only inverted hexagonal (HII) structures in the temperature range 5–70 °C, while the disaccharide compounds adopt only multilamellar structures. Since these and similar glycolipids are frequently found in nature, these data should be of relevance for the function of their host cell membranes.
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  • Ultrastructure and physiochemical properties of CF1F0-ATPase from wheat leaves with atomic force microscopy

    Jie Zhu   Lian-Hong Guo   Guo-Dong Wang  

    The structural preferences of 1,2-dioleoyl-sn-glycerol glycolipids with glucose, galactose, maltose, and cellobiose as sugar head group were investigated under near physiological conditions with Fourier-transform infrared spectroscopy (FT-IR) and synchrotron radiation small-angle X-ray scattering (SAXS). Whereas all glycolipids have a very high fluidity at temperatures above 0 °C, the mono- and disaccharide compounds differ considerably in their aggregate structures. The monosaccharide compounds adopt only inverted hexagonal (HII) structures in the temperature range 5–70 °C, while the disaccharide compounds adopt only multilamellar structures. Since these and similar glycolipids are frequently found in nature, these data should be of relevance for the function of their host cell membranes.
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  • Nanotube Arrays: Joining Copper Oxide Nanotube Arrays Driven by the Nanoscale Kirkendall Effect (Small 15/2013)

    Shu Rong Chun   Wardhana Aji Sasangka   Mei Zhen Ng   Qing Liu   Anyan Du   Jie Zhu   Chee Mang Ng   Zhi Qiang Liu   Sing Yang Chiam and Chee Lip Gan  

    Two stacks of nanotube arrays are joined together, driven by the nanoscale Kirkendall effect. The background images show elemental mapping of copper and oxygen. The contrast of colors shown in the maps represents the intensity of the signals. Such mapping analysis reveals that the interface of the joined nanotubes is copper oxide. On page 2546, C. L. Gan and co-workers demonstrate that the transformation from Cu nanowire arrays into copper oxide nanotube arrays can be coupled with the joining of the stacked chips in a single step process. By controlling the environment, temperature, and duration, joined Cu2O or CuO nanotube stacked chips can be achieved, which serve as a platform for the further development of stacked nanostructured devices.
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  • Facile hydrogen generation using colloidal carbon supported cobalt to catalyze hydrolysis of sodium borohydride

    Jie Zhu   Rong Li   Weiling Niu   Yanjun Wu   Xinglong Gou  

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  • The Thickness of Shock Layers in Liquid Chromatography

    Jie Zhu   Zidu Ma   Georges Guiochon  

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