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Now showing items 1 - 16 of 94

  • SYSTEM, APPARATUS AND METHOD FOR BROADCAST TRANSMISSION OF ETWS MESSAGE

    A system, apparatus and method for broadcast transmission of ETWS message are provided, which is in the ETWS technical field. The system for broadcast transmission of ETWS message includes: the issuing ETWS message entity issues ETWS message; the cell broadcast center packages the ETWS message issued by the issuing ETWS message entity into the CBS message, then sends the CBS message; the mobile management entity transfers the CBS message sent by the cell broadcast center to the wireless access network; the wireless access network receives the CBS message transferred by the mobile management entity, and sends the received CBS message to the user equipment. The system above provides a new technical resolution for transmission of ETWS message, and provides security guarantee to the transmission of ETWS message by use of security mechanism of CBS itself.
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  • METHOD, SYSTEM AND NETWORK DEVICE FOR OBTAINING CELL RESELECTION PRIORITY

    A method, system and network device for obtaining cell reselection priority are disclosed. The method includes: the network side obtains information for calculating priority; the network side determines the special priority list of terminal according to the information, and sends the determined special priority list to the terminal through special signaling, or the terminal obtains part or all of the information for calculating priority from the network side, and determines its own special priority list according to the obtained information, or the network side and terminal determine the special priority list of the terminal according to the information.
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  • METHOD, SYSTEM AND TERMINAL FOR REALIZING SYSTEM INFORMATION SCHEDULE

    A method for realizing system information schedule in a wireless communication system is disclosed in the embodiments of the present invention. The method comprises the following steps of: determining System Frame Number (SFN); setting System Information (SI-x), determining the position at which the SI is located according to the SFN, transmitting all of the SI by distributing all of the SI discontinuously at anytime over the wireless frames. The present invention also provides a system and user terminal for performing system information schedule. With the technical solution supplied by the embodiments of the present invention, it is possible to realize the optimization of the system information schedule, so as to avoid the problems of the network overload and the lower-efficiency of the user data scheduling caused by the transmission of all of the SI at the same time.
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  • SYSTEM INFORMATION SCHEDULING METHOD, DEVICE AND TERMINAL

    A system information scheduling method, device and terminal are disclosed, and the method includes: a terminal obtaining the scheduling information of the most frequently repeated scheduling unit SU-1, and receiving the whole information of the SU-1 according to the scheduling information; the terminal obtaining the dynamical scheduling window information, and receiving information of the other scheduling unit SU-n according to the window information, n>1; the terminal obtaining the mapping information of the system information block SIB and the SU-n from the information of the SU-1, and obtaining all the system information according to the corresponding relationship between the SIB and the SU-n indicated by the mapping information. By using the present method, device and terminal, the UE could receive the SU-1 correctly, and then could receive the whole system information correctly and effectively.
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  • A METHOD FOR HANDLING THE RADIO LINK, A COMMUNICATION METHOD AND DEVICE AFTER THE RADIO LINK FAILING

    A method for handling the radio link, a communication method and device after the radio link failing are provided, the method for handling the radio link includes: the terminal initiates the random access; if the failing times that the terminal initiates the random access exceeds the preconcerted times, the radio link failure is determined. The radio link failure is determined effectively and timely through the method and device provided by this invention.
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  • METHOD AND DEVICE FOR CONFIRMING MAPPING RELATIONSHIP BETWEEN FREQUENCY BAND AND REMOTE ELECTRICAL TILT

    Disclosed are a method and device for confirming a mapping relationship between a frequency band and a remote electrical tilt. The method comprises: sending, by a master device, detection information to an electrical tilt antenna, the detection information carrying a detection method which is currently used by the master device to detect the mapping relationship between a frequency band of a radio-frequency channel and a remote electrical tilt of the electrical tilt antenna, so that the electrical tilt antenna can select the detection method as the current detection method thereof based on the detection information; based on the detection method, detecting, by the master device, the mapping relationship, so that the electrical tilt antenna obtains a detection result based on the current detection method; and obtaining, by the master device, the detection result, and obtaining and recording the mapping relationship based on the detection result. By means of the technical solution, the technical problem in the prior art that electronic devices are mutually incompatible due to the use of different automatic mapping methods is solved.
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  • Zuoyan Zhu, Ph.D., Professor, Institute of Hydrobiology, Chinese Academy of Sciences, Beijing, China

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  • A Novel Dietary Source of EPA and DHA: Metabolic Engineering of an Important Freshwater Species—Common Carp by fat1-Transgenesis

    Zhang, Xiaofan   Pang, Shaochen   Liu, Chengjie   Wang, Houpeng   Ye, Ding   Zhu, Zuoyan   Sun, Yonghua  

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  • Deep sequencing of small RNAs from 11 tissues of grass carp Ctenopharyngodon idella and discovery of sex-related microRNAs

    Chen, Liangming   Huang, Rong   Zhu, Denghui   Yang, Cheng   He, Libo   Li, Yongming   Liao, Lanjie   Zhu, Zuoyan   Wang, Yaping  

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  • Minichromosome maintenance protein 5 homologue in Caenorhabditis elegans plays essential role for postembryonic development.

    Wang, Xiangming   Suh, Christopher   Zhu, Zuoyan   Fan, Qichang  

    Genome duplication is tightly controlled in multicellular organisms to ensure the genome stability. Studies in Saccharomyces cerevisiae and Xenopus show that minichromosome maintenance (MCM) proteins are essential for genome duplication. However, the development role of MCM proteins in multicellular organisms is not well known. MCM5 encodes a member of the MCM2-7 protein family involved in the initiation of DNA replication. The sequences of all Mcm5 homologues from yeast to human are highly conserved and suggest that their functions are also conserved. Here, we isolated the first mutant allele of mcm-5 (fw7) in Caenorhabditis elegans. Homozygous mcm-5 (fw7) mutants from heterozygous parents exhibited variable larval lethality and adult sterility. The postembryonically born neuron number was decreased and also showed aberrant axon morphology. Our study revealed that the losses of neurons in mcm-5 (fw7) mutants were caused by cell cycle defects not by programmed cell death. The examination showed that mcm-5 was widely used for postembryonic development in multiple cells such as seam cells, gonad and intestinal cells. Knockdown of mcm-5 by RNAi caused 98.1% embryonic arrest, suggesting that mcm-5 was also required for embryonic development. After RNAi treatment of the other MCM2-7 family members, we found that they all exhibited similar phenotypes as mcm-5, suggesting that the MCM2-7 family in C. elegans might function associated with cell division as its homologues in S. cerevisiae.
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  • Marcksb plays a key role in the secretory pathway of zebrafish Bmp2b

    Ye, Ding   Wang, Xiaosi   Wei, Changyong   He, Mudan   Wang, Houpeng   Wang, Yanwu   Zhu, Zuoyan   Sun, Yonghua   Mullins, Mary C.  

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  • Characterization and expression of galectin-3 in grass carp (Ctenopharyngodon idella)

    Zhu, Denghui   Huang, Rong   Chu, Pengfei   Chen, Liangming   Li, Yangyu   He, Libo   Li, Yongming   Liao, Lanjie   Zhu, Zuoyan   Wang, Yaping  

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  • Chromosomal deletions and inversions mediated by TALENs and CRISPR/Cas in zebrafish.

    Xiao, An   Wang, Zhanxiang   Hu, Yingying   Wu, Yingdan   Luo, Zhou   Yang, Zhipeng   Zu, Yao   Li, Wenyuan   Huang, Peng   Tong, Xiangjun   Zhu, Zuoyan   Lin, Shuo   Zhang, Bo  

    Customized TALENs and Cas9/gRNAs have been used for targeted mutagenesis in zebrafish to induce indels into protein-coding genes. However, indels are usually not sufficient to disrupt the function of non-coding genes, gene clusters or regulatory sequences, whereas large genomic deletions or inversions are more desirable for this purpose. By injecting two pairs of TALEN mRNAs or two gRNAs together with Cas9 mRNA targeting distal DNA sites of the same chromosome, we obtained predictable genomic deletions or inversions with sizes ranging from several hundred bases to nearly 1 Mb. We have successfully achieved this type of modifications for 11 chromosomal loci by TALENs and 2 by Cas9/gRNAs with different combinations of gRNA pairs, including clusters of miRNA and protein-coding genes. Seven of eight TALEN-targeted lines transmitted the deletions and one transmitted the inversion through germ line. Our findings indicate that both TALENs and Cas9/gRNAs can be used as an efficient tool to engineer genomes to achieve large deletions or inversions, including fragments covering multiple genes and non-coding sequences. To facilitate the analyses and application of existing ZFN, TALEN and CRISPR/Cas data, we have updated our EENdb database to provide a chromosomal view of all reported engineered endonucleases targeting human and zebrafish genomes.
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  • RNA-seq profiles from grass carp tissues after reovirus (GCRV) infection based on singular and modular enrichment analyses.

    Shi, Mijuan   Huang, Rong   Du, Fukuan   Pei, Yongyan   Liao, Lanjie   Zhu, Zuoyan   Wang, Yaping  

    Hemorrhagic disease of the grass carp, Ctenopharyngodon idella, is a fatal disease in fingerlings and yearlings caused by a reovirus, GCRV. RNA-seq data from four diseased grass carp tissues (gill, intestine, liver and spleen) were obtained at 2h before and six times after (2h, 24h, 48h, 72h, 96h and 120h) GCRV challenge. A total of 7.25=C2=B10.18 million (M) clean reads and 3.53=C2=B10.37M unique reads were obtained per RNA-seq analysis. Compared with controls, there were 9060 unique differentially expressed genes (DEGs) in the four tissues at the six time points post-GCRV challenge. Hierarchical clustering analysis of the DEGs showed that the data from the six time points fell into three branches: 2h, 24h/48h, and 72h/96h/120h. Singular (SEA) and modular enrichment analyses of DEGs per RNA-seq dataset were performed based on gene ontology. The results showed that immune responses occurred in all four tissues, indicating that GCRV probably does not target any tissue specifically. Moreover, during the course of disease, disturbances were observed in lipid and carbohydrate metabolism in each of the organs. SEA of DEGs based on the Kyoto Encyclopedia of Genes and Genomes database was also performed, and this indicated that the complement system and cellular immunity played an important role during the course of hemorrhagic disease. The qPCR of pooled samples of duplicate challenge experiment were used to confirm our RNA-seq approach. Copyright =C2=A9 2014 Elsevier Ltd. All rights reserved.
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  • Molecular cloning and preliminary functional analysis of six RING-between-ring (RBR) genes in grass carp (Ctenopharyngodon idellus)

    Luo, Lifei   Zhu, Denghui   Huang, Rong   Xiong, Lv   Mehjabin, Rumana   He, Libo   Liao, Lanjie   Li, Yongming   Zhu, Zuoyan   Wang, Yaping  

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  • Toll-like receptor 4 signaling pathway can be triggered by grass carp reovirus and Aeromonas hydrophila infection in rare minnow Gobiocypris rarus

    Su, Jianguo   Yang, Chunrong   Xiong, Feng   Wang, Yaping   Zhu, Zuoyan  

    Toll-like receptor 4 (TLR4) is critical for LPS recognition and cellular responses. It also recognizes some viral envelope proteins. Detection mostly results in the inflammation rather than specific antiviral responses. However, it's unclear in fish. In this report, a TLR4 gene (named as GrTLR4b) was cloned and characterized from rare minnow Gobiocypris rarus. The full length of GrTLR4b cDNA consists of 2766 nucleotides and encodes a polypeptide of 818 amino acids with an estimated molecular mass of 94,518 Da and a predicted isoelectric point of 8.41. The predicted amino acid sequence comprises a signal peptide, six leucine-rich repeat (LRR) motifs, one leucine-rich repeat C-terminal (LRRCT) motif, followed by a transmembrane segment of 23 amino acids, and a cytoplasmic region of 167 amino acids containing one Toll - interleukin 1 - receptor (TIR) motif. It's closely similar to the zebrafish (Danio rerio) TLR4b amino acid sequence with an identity of 77%. Quantitative RT-PCR analysis showed GrTLR4b mRNA was constitutive expression in gill, heart, intestine, kidney, liver, muscle and spleen tissues in healthy animals and up-regulated by viruses and bacteria. After being infected by grass carp reovirus or Aeromonas hydrophila, GrTLR4b expressions were up-regulated from 24 h post-injection and lasted until the fish became moribund (P < 0.05). These data implied that TLR4 signaling pathway could be activated by both viral and bacterial infection in rare minnow. (C) 2009 Elsevier Ltd. All rights reserved.
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