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Now showing items 33 - 43 of 43

  • Characterization of class B scavenger receptor type 1 (SRB1) in turbot (Scophthalmus maximus L.)

    Li, Chao   Ge, Xuefeng   Su, Baofeng   Fu, Qiang   Wang, Beibei   Liu, Xiaoli   Ren, Yichao   Song, Lin   Yang, Ning  

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  • Early mucosal responses in blue catfish (Ictalurus furcatus) skin to Aeromonas hydrophila infection

    Li, Chao   Beck, Benjamin   Su, Baofeng   Terhune, Jeffery   Peatman, Eric  

    Bacterial pathogens are well-equipped to detect, adhere to, and initiate infection in their finfish hosts. The mucosal surfaces of fish, such as the skin, function as the front line of defense against such bacterial insults that are routinely encountered in the aquatic environment. While recent progress has been made, and despite the obvious importance of mucosal surfaces, the precise molecular events that occur soon after encountering bacterial pathogens remain unclear. Indeed, these early events are critical in mounting appropriate responses that ultimately determine host survival or death. In the present study, we investigated the transcriptional consequences of a virulent Aeromonas hydrophila challenge in the skin of blue catfish, Ictalurus furcatus. We utilized an 8 x 60K Agilent microarray to examine gene expression profiles at key early timepoints following challenge (2 h, 12 h, and 24 h). A total of 1155 unique genes were significantly altered during at least one timepoint. We observed dysregulation in a number of genes involved in diverse pathways including those involved in antioxidant responses, apoptosis, cytoskeletal rearrangement, immunity, and extracellular matrix protein diversity and regulation. Taken together, A. hydrophila coordinately modulates mucosal factors across numerous cellular pathways in a manner predicted to enhance its ability to adhere to and infect the blue catfish host. (C) 2013 Elsevier Ltd. All rights reserved.
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  • Interaction of diet and the masou salmon Delta5-desaturase transgene on Delta6-desaturase and stearoyl-CoA desaturase gene expression and N-3 fatty acid level in common carp (Cyprinus carpio).

    Cheng, Qi   Su, Baofeng   Qin, Zhenkui   Weng, Chia-Chen   Yin, Fang   Zhou, Yangen   Fobes, Michael   Perera, Dayan A   Shang, Mei   Soller, Fabio   Shi, Zhiyi   Davis, Allen   Dunham, Rex A  

    The masou salmon Delta5-desaturase-like gene (D5D) driven by the common carp beta-actin promoter was transferred into common carp (Cyprinus carpio) that were fed two diets. For P1 transgenic fish fed a commercial diet, Delta6-desaturase-like gene (D6D) and stearoyl-CoA desaturase (SCD) mRNA levels in muscle were up-regulated (P<0.05) 12.7- and 17.9-fold, respectively, and the D6D mRNA level in the gonad of transgenic fish was up-regulated 6.9-fold (P<0.05) compared to that of non-transgenic fish. In contrast, D6D and SCD mRNA levels in transgenic fish were dramatically down-regulated (P<0.05), 50.2- and 16.7-fold in brain, and 5.4- and 2.4-fold in liver, respectively, in comparison with those of non-transgenic fish. When fed a specially formulated diet, D6D and SCD mRNA levels in muscle of transgenic fish were up-regulated (P<0.05) 41.5- and 8.9-fold, respectively, and in liver 6.0- and 3.3-fold, respectively, compared to those of non-transgenic fish. In contrast, D6D and SCD mRNA levels in the gonad of transgenic fish were down-regulated (P<0.05) 5.5- and 12.4-fold, respectively, and D6D and SCD mRNA levels in the brain were down-regulated 14.9- and 1.4-fold (P<0.05), respectively, compared to those of non-transgenic fish. The transgenic common carp fed the commercial diet had 1.07-fold EPA, 1.12-fold DPA, 1.07-fold DHA, and 1.07-fold higher observed total omega-3 fatty acid levels than non-transgenic common carp. Although these differences were not statistically different (P>0.05), there were significantly (P<0.10) higher omega-3 fatty acid levels when considering the differences for all of the individual omega-3 fatty acids. The genotype*diet interactions observed indicated that the potential of desaturase transgenesis cannot be realized without using a well-designed diet with the needed amount of substrates. =20
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  • Suppression and restoration of primordial germ cell marker gene expression in channel catfish, Ictalurus punctatus, using knockdown constructs regulated by copper transport protein gene promoters: Potential for reversible transgenic sterilization.

    Su, Baofeng   Shang, Mei   Grewe, Peter M   Patil, Jawahar G   Peatman, Eric   Perera, Dayan A   Cheng, Qi   Li, Chao   Weng, Chia-Chen   Li, Ping   Liu, Zhanjiang   Dunham, Rex A  

    Complementary DNA overexpression and short hairpin RNA interference approaches were evaluated for decreasing expression of primordial germ cell (PGC) marker genes and thereby sterilizing channel catfish, Ictalurus punctatus, by delivering knockdown constructs driven by a constitutive promoter from yeast and a copper transport protein gene into fish embryos by electroporation. Two PGC marker genes, nanos and dead end, were the target knockdown genes, and their expressions, along with that of an off-target gene, vasa, were evaluated temporally using real-time polymerase chain reaction. Copper sulfate was evaluated as a repressor compound. Some of the constructs knocked down PGC marker gene expression, and some of the constructs were partially repressed by application of 0.1-ppm copper sulfate. When the rate of sexual maturity was compared for three-year-old broodfish that had been exposed to the sterilizing constructs during embryologic development and controls that had not been exposed, several treatments had reduced sexual maturity for the exposed fish. Of two promoter systems evaluated, the one which had been designed to be less sensitive to copper generally was more effective at achieving sterilization and more responsive to repression. Knockdown constructs based on 3' nanos short hairpin RNA interference appeared to result in the best repression and restoration of normal sexual maturity. We conclude that these copper-based systems exhibited good potential for repressible transgenic sterilization. Optimization of this system could allow environmentally safe application of transgenic technology and might be applicable to other applications for aquatic organisms. Copyright =C2=A9 2015 Elsevier Inc. All rights reserved.
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  • Effects of Cecropin Transgenesis and Interspecific Hybridization on the Resistance to Ichthyophthirius multifiliis in Channel Catfish and Female Channel Catfish × Male Blue Catfish Hybrids

    Elaswad, Ahmed   Khalil, Karim   Ye, Zhi   Alsaqufi, Ahmed   Abdelrahman, Hisham   Su, Baofeng   Perera, Dayan A.   Dong, Sheng   Abass, Nermeen   Dunham, Rex  

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  • Galectins in channel catfish,Ictalurus punctatus:Characterization and expression profiling in mucosal tissues

    Zhou, Shun   Zhao, Honggang   Thongda, Wilawan   Zhang, Dongdong   Su, Baofeng   Yu, Dan   Peatman, Eric   Li, Chao  

    Galectins, a family of beta-galactoside-binding lectins with conserved CRDs, which can recognize the glycans on the surface of viruses, bacteria and protozoan parasites, are emerging as key players in many important pathological processes, including acute and chronic inflammatory diseases, autoimmunity and apoptosis. Although galectins have attracted great interest in mammals, they are still poorly characterized in teleost. Previously, several studies have reported their high expression levels in mucosal tissues before and post infection. Given the important roles for galectins in mucosal immunity, therefore, we characterized the galectin gene family and profiled family member expression after challenge with two different Gram-negative bacterial pathogens. Here, twelve galectins genes were captured in channel catfish (Ictalurus punctatus), and phylogenetic analysis showed the strongest relationship to zebrafish and salmon, which is consistent with their phylogenetic relationships. Furthermore, the galectin genes were widely expressed in catfish tissues, while most of the galectin genes were strongly expressed in mucosal tissues (skin, gill and intestine). In addition, the expression profiles of galectins after bacterial infection varied depending on both pathogen and tissue type, suggesting that galectins may exert disparate functions or exhibit distinct tissue-selective roles in the host immune response to bacterial pathogens. Further studies are needed, however, to expand functional characterization and examine whether galectins may also play additional physiological roles in catfish immunity. (C) 2016 Elsevier Ltd. All rights reserved.
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  • Effects of multiple handstrippings of channel catfish (Ictalurus punctatus) females induced to ovulate with carp pituitary extract on production of channel catfish female x blue catfish (I-furcatus) male hybrid fry

    Kim, Soonhag   Su, Baofeng   Argue, Brad J.   Ligeon, Carel   Ramboux, Anne C.  

    Channel catfish (Ictalurus punctatus) females were injected with carp pituitary extract (CPE) and were multiple handstripped three times to harvest eggs. Eggs were fertilized with sperm from blue catfish (I. furcatus) to produce channel-blue hybrid (CB hybrid) embryos and fry. A higher number of females yielded eggs (P<.05) during the first stripping attempt (90.3 +/- 1.1% [mean +/- SE]) than during the second (67.6 +/- 2.1%) and third attempts (7.6 +/- 0.5%). Significant differences also were observed for the number of eggs/kg stripped between the first (5,471 +/- 225) and second (2,598 +/- 97) and the first and third (1,302 +/- 46) strippings. Percent fertilization (71.5 +/- 2.7 %) was higher (P<.05) for the first stripping than the second and third strippings, but the second stripping (58.2 +/- 1.8%) was not different from the third (51.7 +/- 1.6%). The hatching percent of embryos did not vary among the three times strippings. The fry/kg female body weight produced was higher (P<.05) for the first (1,399 +/- 112) than the second (471 +/- 26) and third strippings (28 +/- 2). It is productive to strip the females a second time, as fry production can be increased by 33.7% by waiting 5h and harvesting the additional eggs ready at that time. The time and effort for a third stripping is not worthwhile because of the low percentage of females releasing eggs at that time and the low fry output.
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  • The relationship between channel catfish female body weight and relative fecundity and fry production when induced to ovulate with carp pituitary extract and fertilized with blue catfish sperm

    Su, Baofeng   Kim, Soonhag   Argue, Brad J.   Ligeon, Carel   Ramboux, Anne C.  

    Little is known regarding the correlation between channel catfish female body weight and relative fecundity and fry production when the females are induced to ovulate with carp pituitary extract and fertilized with blue catfish sperm. A total of 145 female channel catfish were used in the early, middle, and late spawning season. Female body weight of channel catfish had negative correlations with relative fecundity (r=3D-0.33 and -0.25, P<.05; r=3D-0.23, P>.05) and before the late spawning season with fry/kg (r=3D-0.21, P>.05; r=3D-0.30, P<.05). Eggs/kg female body weight and fry/kg female body weight followed a parallel pattern throughout the season, illustrating the logical relationship between relative fecundity and fry output. The size of females used is a consideration to maximize hybrid catfish embryo production during the early and middle spawning season but not late in the spawning season. However, these correlations are relatively small even though significant; thus a dramatic change in fry production would not be predicted. Since reproductive relationships can change during the spawning season, conclusions and recommendations could be flawed if data are not collected periodically during an entire spawning season.
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  • Characterization and initial functional analysis of cathepsin K in turbot (Scophthalmus maximus L.)

    Tian, Mengyu   Cao, Min   Zhang, Lu   Fu, Qiang   Yang, Ning   Tan, Fenghua   Song, Lin   Su, Baofeng   Li, Chao  

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  • Dynamics of MiRNA Transcriptome in Turbot (Scophthalmus maximus L.) Intestine Following Vibrio anguillarum Infection

    Gao, Chengbin   Cai, Xin   Fu, Qiang   Yang, Ning   Song, Lin   Su, Baofeng   Tan, Fenghua   Liu, Baining   Li, Chao  

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  • Editing of the Luteinizing Hormone Gene to Sterilize Channel Catfish,Ictalurus punctatus,Using a Modified Zinc Finger Nuclease Technology with Electroporation

    Qin, Zhenkui   Li, Yun   Su, Baofeng   Cheng, Qi   Ye, Zhi   Perera, Dayan A.   Fobes, Michael   Shang, Mei   Dunham, Rex A.  

    Channel catfish (Ictalurus punctatus) is the most important freshwater aquaculture species in the USA. Genetically enhanced fish that are sterile could both profit the catfish industry and reduce potential environmental and ecological risks. As the first step to generate sterile channel catfish, three sets of zinc finger nuclease (ZFN) plasmids targeting the luteinizing hormone (LH) gene were designed and electroporated into one-cell embryos, different concentrations were introduced, and the Cel-I assay was conducted to detect mutations. Channel catfish carrying the mutated LH gene were sterile, as confirmed by DNA sequencing and mating experiments. The overall mutation rate was 19.7 % for 66 channel catfish, and the best treatment was ZFN set 1 at the concentration 25 mu g/ml. To our knowledge, this is the first instance of gene editing of fish via plasmid introduction instead of mRNA microinjection. The introduction of the ZFN plasmids may have reduced mosaicism, as mutated individuals were gene edited in every tissue evaluated. Apparently, the plasmids were eventually degraded without integration, as they were not detectable in mutated individuals using PCR. Carp pituitary extract failed to induce spawning and restoration of fertility, indicating the need for developing other hormone therapies to achieve reversal of sterility upon demand. This is the first sterilization achieved using ZFN technology in an aquaculture species and the first successful gene editing of channel catfish. Our results will help understand the roles of the LH gene, purposeful sterilization of teleost fishes, and is a step towards control of domestic, hybrid, exotic, invasive, and transgenic fishes.
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