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Now showing items 129 - 144 of 336

  • Identification and initial functional characterization of lysosomal integral membrane protein type 2 (LIMP-2) in turbot (Scophthalmus maximus L.)

    Tan, Fenghua   Cao, Min   Ge, Xuefeng   Li, Chao   Tian, Mengyu   Zhang, Lu   Fu, Qiang   Song, Lin   Yang, Ning  

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  • Composition dependent microstructure evolution, activation and de-/hydrogenation properties of Mg–Ni–La alloys

    Guo, Fenghai   Zhang, Tiebang   Shi, Limin   Song, Lin  

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  • Corrosion resistance and interfacial morphologies of a high Nb-containing TiAl alloy with and without thermal barrier coatings in molten salts

    Zhang, Kun   Zhang, Tiebang   Zhang, Xuhu   Song, Lin  

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  • New insights into high-temperature deformation and phase transformation mechanisms of lamellar structures in high Nb-containing TiAl alloys

    Song, Lin   Appel, Fritz   Wang, Li   Oehring, Michael   Hu, Xingguo   Stark, Andreas   He, Junyang   Lorenz, Uwe   Zhang, Tiebang   Lin, Junpin   Pyczak, Florian  

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  • Autophagy-deficient mice are more susceptible to engrafted leukemogenesis

    Ge, Chaorong   An, Ni   Li, Lei   Wei, Wen   Ji, Li   Yuan, Na   Fang, Yixuan   Xu, Li   Song, Lin   Zhang, Jingyi   Song, Chenglin   Wang, Jianrong   Zhang, Suping  

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  • Magnetic nanoparticle-containing soft-hard diblock copolymer films with high order

    Xia, Senlin   Song, Lin   Koerstgens, Volker   Opel, Matthias   Schwartzkopf, Matthias   Roth, Stephan V.   Mueller-Buschbaum, Peter  

    For sensor applications, superparamagnetic anisotropy is an indispensable property, which is typically achieved by employing an external field to guide the arrangement of magnetic nanoparticles (NPs). In the present investigation, the diblock copolymer polystyrene-block-poly(N-isopropylacrylamide) (PS-b-PNIPAM) is printed as a template to localize magnetic iron oxide NPs without any external field. Via microphase separation, cylindrical nanostructures of PS in a PNIPAM matrix are obtained, aligned perpendicular to the substrate. Since the magnetite NPs (Fe3O4) are functionalized with hydrophobic organic chains showing affinity to the PS blocks, they can selectively aggregate inside the PS cylinders. Moreover, solvent vapor annealing allows the achievement of nanostructures inside the hybrid system with a very high order, even at a high NP loading. Therefore, NPs can accumulate within PS domains to form perpendicularly aligned aggregates with high periodicity. The magnetic properties of the hybrid films are determined at various temperatures in two orthogonal directions (with PS cylinders vertical and parallel to the applied magnetic field). All hybrid films show superparamagnetism and a remarkable magnetic anisotropy is achieved at certain NP concentrations. This investigation shows a facile route to prepare superparamagnetic films with magnetic anisotropy and offers a novel possibility to future magnetic sensor fabrication.
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  • Determination of butenafine hydrochloride in human plasma by liquid chromatography electrospray ionization-mass spectrometry following its topical administration in human subjects

    Song, Lin   Jiang, Xuehua  

    An HPLC/MS/MS method for determination of butenafine hydrochloride in human plasma with testosterone propionate as the internal standard (IS) was developed and validated. Plasma samples were extracted with an n-hexane/diethyl ether (1:2, v/v) mixture and separated using a C-18 column by a gradient elution with the mobile phase containing acetonitrile and 5 mM ammonium acetate buffer. Quantification was performed using multiple reaction monitoring (MRM) mode with transition of m/z 318.4 -> 141.0 for butenafine hydrochloride and m/z 345.5 -> 97.0 for testosterone propionate (IS). This method was validated in terms of specificity, linearity, precision, accuracy, and stability. The lower limit of quantification (LLOQ) of this method was 0.0182 ng/ml and the calibration curve was linear over the 0.0182-1.82 ng/ml. The intra- and inter-run coefficient of variance was less than 11.53% and 10.07%, respectively. The samples were stable under all the tested conditions. The method was successfully applied to study the pharmacokinetics of butenafine hydrochloride in healthy Chinese volunteers following its topical administration. (C) 2011 Elsevier B.V. All rights reserved.
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  • Mesenchymal stem cells protect cigarette smoke-damaged lung and pulmonary function partly via VEGF-VEGF receptors

    Guan, Xiao-Jun   Song, Lin   Han, Feng-Feng   Cui, Zhi-Lei   Chen, Xi   Xu, Wei-Guo  

    Progressive pulmonary inflammation and emphysema have been implicated in the progression of chronic obstructive pulmonary disease (COPD), while current pharmacological treatments are not effective. Transplantation of bone marrow mesenchymal stem cells (MSCs) has been identified as one such possible strategy for treatment of lung diseases including acute lung injury (ALI) and pulmonary fibrosis. However, their role in COPD still requires further investigation. The aim of this study is to test the effect of administration of rat MSCs (rMSCs) on emphysema and pulmonary function. To accomplish this study, the rats were exposed to cigarette smoke (CS) for 11 weeks, followed by administration of rMSCs into the lungs. Here we show that rMSCs infusion mediates a down-regulation of pro-inflammatory mediators (TNF-a, IL-1 beta, MCP-1, and IL-6) and proteases (MMP9 and MMP12) in lung, an up-regulation of vascular endothelial growth factor (VEGF), VEGF receptor 2, and transforming growth factor (TGF beta-1), while reducing pulmonary cell apoptosis. More importantly, rMSCs administration improves emphysema and destructive pulmonary function induced by CS exposure. In vitro co-culture system study of human umbilical endothelial vein cells (EA.hy926) and human MSCs (hMSCs) provides the evidence that hMSCs mediates an anti-apoptosis effect, which partly depends on an up-regulation of VEGF. These findings suggest that MSCs have a therapeutic potential in emphysematous rats by suppressing the inflammatory response, excessive protease expression, and cell apoptosis, as well as up-regulating VEGF, VEGF receptor 2, and TGF beta-1. J. Cell. Biochem. 114: 323335, 2013. (c) 2012 Wiley Periodicals, Inc.
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  • Dehydrogenation steps and factors controlling desorption kinetics of a Mg-Ce hydrogen storage alloy

    Xie, Lishuai   Li, Jinshan   Zhang, Tiebang   Song, Lin  

    Experimentally systematical comparisons are carried out in this work to clarify dehydrogenation steps of Mg-based hydrogen storage alloys during the overall desorption process. Different forms of MgH2-CeH2.73 composite powders are prepared by high energy ball milling, partial dehydrogenation and annealing. For partially dehydrogenated samples, the desorption temperature and desorption activation energy decrease significantly considering the fact that primary-precipitated metal Mg phase on the surface of MgH2 can act as nucleate precursors. No significant difference in isothermal desorption kinetics is observed for MgH2-CeH2.73 powders with different grain sizes. However, particle size reduction facilitates desorption at temperatures below 300 degrees C. As minor Ni is distributed on the surface, both onset and peak temperatures in thermal desorption decrease for MgH2-CeH2.73 composite. The reduced activation energy by Ni addition is comparable to the value caused by partial dehydrogenation. Recombination of hydrogen atoms plays an important role during dehydrogenation. The obtains in this work can be expected to provide guidelines to improve desorption kinetics of Mg-based alloys. (C) 2017 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.
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  • Surface-modified GVs as nanosized contrast agents for molecular ultrasound imaging of tumor

    Wang, Guohao   Song, Lin   Hou, Xuandi   Kala, Shashwati   Wong, Kin Fung   Tang, Liya   Dai, Yunlu   Sun, Lei  

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  • Modeling Thin Film Solar Cells: From Organic to Perovskite

    Li, Deli   Song, Lin   Chen, Yonghua   Huang, Wei  

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  • Sulfated Polysaccharides Isolated from Cloned Grateloupia filicina and Their Anticoagulant Activity

    Chen, Xiaolin   Yang, Shengfeng   Wang, Jinxia   Song, Lin   Xing, Ronge   Liu, Song   Yu, Huahua   Li, Pengcheng  

    Sulfated polysaccharides (GSP) were isolated from the cloned Grateloupia filicina which was cultured in Jiaozhou Bay, Qingdao, China. The yield of GSP was 15.75%. The total sugar and sulfate were 40.90 and 19.89%, respectively. And the average molecular weight was 11.7 KDa. The results of neutral sugar analysis showed that GSP was mainly sulfated polysaccharides of galactose. The experiments for activated partial thromboplastin time (APTT), prothrombin time (PT), and thrombin time (TT) anticoagulant assays in vitro indicated that GSP was a good potential anticoagulant. Therefore, this study supplied new thought for the cloned Grateloupia filicina exploitation of high-value products.
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  • Characterization of zebrafish Pax1b and Pax9 in fin bud development.

    Chen, Xuemei   Huang, Huizhe   Wang, Hua   Guo, Fengjin   Du, Xiaogang   Ma, Linqiang   Zhao, Liang   Pan, Zhuma   Gui, Haibo   Yuan, Taixian   Liu, Xin   Song, Lin   Wang, Yiquan   He, Junling   Lei, Han   Gao, Rui  

    Both Pax1 and Pax9 belong to the important paired box gene family (PAX), which mainly participates in animal development and sclerotome differentiation. To date, the precise molecular mechanism and related signaling pathway of Pax1 remain unclear. In our study, microinjection of morpholino- (MO-) modified antisense oligonucleotides against pax1b induced pectoral fin bud defects. Furthermore, we demonstrate that the phenotypes caused by the knockdown of Pax1b in zebrafish could not be phenocopied by pax9 MO and could not be rescued by either Pax1a or Pax9 overexpression. We further find that Pax1b affects the expression of col2a1, Uncx4.1, Noggin3, and aggrecan, confirming the role of Pax1b in chondrocyte differentiation and bone maturation. Moreover, we identify an interaction between PAX1 and FOXO1 and find that the interaction was enhanced under hypoxia stress. Together, this evidence for cell death caused by pax1b knockdown provides new insight into the role of the Pax protein family in cell fate determination and tissue specification. =20
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  • Han\"guk Kwahaksa Hakhoe-ji: Journal of the Korean History of Science Societyby Song Sang-yong;Historia Scientiarum: The International Journal of the History of Science Society of Japanby Jun Fujimura;Kagakusi Kenkyu: Journal of History of Science, Japanby Ichiro Yabe;Ziran Kexueshi Yanjiu (Studies in the History of Natural Sciences)by Lin Wenjao

    Review by: Yung Sik Kim  

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  • Crc is involved in catabolite repression control of the bkd operons of Pseudomonas putida and Pseudomonas aeruginosa

    Hester, Kathryn L.   Lehman, Jodi   Najar, Fares   Song, Lin   Roe, Bruce A.   MacGregor, Carolyn H.   Hager, Paul W.   Phibbs, Paul V., Jr.   Sokatch, John R.  

    Crc (catabolite repression control) protein of Pseudomonas aeruginosa has shown to be involved in carbon regulation of several pathways. In this study, the role of Crc in catabolite repression control has been studied in Pseudomonas putida. The bkd operons of P. putida and P. aeruginosa encode the inducible multienzyme complex branched-chain keto acid dehydrogenase, which is regulated in both species by catabolite repression. We report here that this effect is mediated in both species by Crc. A 13-kb cloned DNA fragment containing the P. putida crc gene region was sequenced. Crc regulates the expression of branched-chain keto acid dehydrogenase, glucose-6-phosphate dehydrogenase, and amidase in both species but not urocanase, although the carbon sources responsible for catabolite repression in the two species differ. Transposon mutants affected in their expression of BkdR, the transcriptional activator of the bkd operon, were isolated and identified as crc and vacB (rnr) mutants. These mutants suggested that catabolite repression in pseudomonads might, in part, involve control of BkdR levels.
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  • SUMOylation Negatively Regulates Angiogenesis by Targeting Endothelial NOTCH Signaling

    Zhu, Xiaolong   Ding, Sha   Qiu, Cong   Shi, Yanna   Song, Lin   Wang, Yueyue   Wang, Yuewen   Li, Jinying   Wang, Yiran   Sun, Yi   Qin, Lingfeng   Chen, Jun   Simons, Michael   Min, Wang   Yu, Luyang  

    Rationale: The highly conserved NOTCH (neurogenic locus notch homolog protein) signaling pathway functions as a key cell-cell interaction mechanism controlling cell fate and tissue patterning, whereas its dysregulation is implicated in a variety of developmental disorders and cancers. The pivotal role of endothelial NOTCH in regulation of angiogenesis is widely appreciated; however, little is known about what controls its signal transduction. Our previous study indicated the potential role of post-translational SUMO (small ubiquitin-like modifier) modification (SUMOylation) in vascular disorders. Objective: The aim of this study was to investigate the role of SUMOylation in endothelial NOTCH signaling and angiogenesis. Methods and Results: Endothelial SENP1 (sentrin-specific protease 1) deletion, in newly generated endothelial SENP1 (the major protease of the SUMO system)-deficient mice, significantly delayed retinal vascularization by maintaining prolonged NOTCH1 signaling, as confirmed in cultured endothelial cells. An in vitro SUMOylation assay and immunoprecipitation revealed that when SENP1 associated with N1ICD (NOTCH1 intracellular domain), it functions as a deSUMOylase of N1ICD SUMOylation on conserved lysines. Immunoblot and immunoprecipitation analyses and dual-luciferase assays of natural and SUMO-conjugated/nonconjugated NOTCH1 forms demonstrated that SUMO conjugation facilitated NOTCH1 cleavage. This released N1ICD from the membrane and stabilized it for translocation to the nucleus where it functions as a cotranscriptional factor. Functionally, SENP1-mediated NOTCH1 deSUMOylation was required for NOTCH signal activation in response to DLL4 (Delta-like 4) stimulation. This in turn suppressed VEGF (vascular endothelial growth factor) receptor signaling and angiogenesis, as evidenced by immunoblotted signaling molecules and in vitro angiogenesis assays. Conclusions: These results establish reversible NOTCH1 SUMOylation as a regulatory mechanism in coordinating endothelial angiogenic signaling; SENP1 acts as a critical intrinsic mediator of this process. These findings may apply to NOTCH-regulated biological events in nonvascular tissues and provide a novel therapeutic strategy for vascular diseases and tumors.
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