The present invention encompasses prostaglandin E2 (PGE2) binding proteins. The invention relates to antibodies that are wild-type, chimeric, CDR grafted and humanized. Preferred antibodies have high affinity for prostaglandin E2 and neutralize prostaglandin E2 activity in vitro and in vivo. An antibody of the invention can be a full-length antibody, or an antigen-binding portion thereof. Methods of making and methods of using the antibodies of the invention are also provided. The antibodies, or antigen-binding portions, of the invention are useful for detecting prostaglandin E2 and for inhibiting prostaglandin E2 activity, e.g., in a human subject suffering from a disorder in which prostaglandin E2 activity is detrimental.
Rong-Rong Zhu
Shi-Long Wang
Rui Zhang
Xiao-Yu Sun
Si-De Yao
Abstract TiO2 has been tested to be toxic to DNA under the photo-irradiation of ultraviolet A (UVA). However, in the dark conditions, after several days of treatment with TiO2 in aqueous solution, the interaction between TiO2 and two types of DNA was detected and the mechanisms were studied by the methods of gel-electrophoresis, IR spectroscopy and TEM. The results showed that the DNA would be bound to TiO2; the ratio of binding was related to the concentration and the treating time; the mechanism of binding is related to phosphate groups of DNA. Besides, DNA with different structure showed different degree of binding. These findings showed a new possible way through which the TiO2 nanoparticles interact with DNA.
Xiao-Lie He
Li Yang
Zhao-Jie Wang
Rui-Qi Huang
Rong-Rong Zhu
Li-Ming Cheng
Dexanabinol（HU-211） is an artificially synthesized cannabinoid derivative that exerts neuroprotective effects through anti-inflammatory and antioxidant effects.Curcumin exhibits antidepressant effects in the treatment of major depressive disorder.To investigate the antidepressant effects of solid lipid nanoparticles loaded with both curcumin and dexanabinol,and the underlying mechanisms associated with this combination,we established wild-type(CBR1–/––/–
Xiao-Lie He
Li Yang
Zhao-Jie Wang
Rui-Qi Huang
Rong-Rong Zhu
Li-Ming Cheng
Dexanabinol(HU-211)is an artificially synthesized cannabinoid derivative that exerts neuroprotective effects through anti-inflammatory and antioxidant effects.Curcumin exhibits antidepressant effects in the treatment of major depressive disorder.To investigate the antidepressant effects of solid lipid nanoparticles loaded with both curcumin and dexanabinol,and the underlying mechanisms associated with this combination,we established wild-type(CBR1~(+/+))and cannabinoid receptor 1(CBR1)knockout(CBR1~(–/–))mouse models of major depressive disorder,through the intraperitoneal injection of corticosterone,for 3 successive days,followed by treatment with intraperitoneal injections of solid lipid nanoparticles loading with curcumin(20 mg/kg)and dexanabinol(0.85 mg/kg),for 2 successive days.Our results revealed that solid lipid nanoparticle loading with curcumin and dexanabinol increased the m RNA and protein expression levels of the mature neuronal markers neuronal nuclei,mitogen-activated protein 2,and neuron-specific beta-tubulin III,promoted the release of dopamine and norepinephrine,and increased the m RNA expression of CBR1 and the downstream genes Rasgef1c and Egr1,and simultaneously improved rat locomotor function.However,solid lipid nanoparticles loaded with curcumin and dexanabinol had no antidepressant effects on the CBR1~(–/–)mouse models of major depressive disorder.This study was approved by the Institutional Ethics Committee of Tongji Hospital of Tongji University,China(approval No.2017-DW-020)on May 24,2017.
Rong-Rong Zhu
Wen-Rui Wang
Xiao-Yu Sun
Hui Liu
Shi-Long Wang
In this study, the binding and enzyme activity inhibitory effect of nano-TiO2 on pepsin was explored compared with micro-TiO2. Nano-TiO2 was about 60 nm and micro-TiO2 was about 200 nm, both round in shape. The activity of pepsin was depressed significantly by nano-TiO2 comparing to micro-ones. The results of UV spectrometry, HPLC, SDS–PAGE and CD assay proved that micro-TiO2 has only physical absorption effect on pepsin, but no impairment on primary sequences or secondary structure. However, nano-TiO2 had coordination interaction with pepsin besides physical binding effect. The secondary structure of pepsin was unfolded with the treatment of nano-TiO2 at pH 6.5 and pH 3.53, which might consequently affect the β-hairpin loop that protects the active center of pepsin, and then reduce the enzyme activity. Furthermore, the thermodynamic mechanisms of interaction between nano-TiO2 and pepsin were explored by fluorescence spectrum and ITC analysis. According to the results of thermodynamic analysis, the K value was 3.64 × 106, stoichiometry (Npepsin:nano-TiO2) was 3.04 × 103, the total ΔH was −2277 cal/mol, ΔS was 22.7 cal/(K mol), therefore the nano-TiO2–pepsin interaction is spontaneous. The depression of activity and the unfolding of secondary structure of pepsin were resulted from non-covalent reactions, including electrostatic force and hydrophobic binding. This work studied the different inhibitory effects and revealed mechanisms of the interaction between micro/nano-TiO2 and pepsin, and provided a useful approach for evaluating the health risk of nano-materials on level of proteins.
Rong-Rong Zhu;Tao Wang;Zhen Zhang;Suo-Shu Zhang;Yan-Ru Zhao;Rui-Dan Bao;Lei Jia;Lin Du;Qi-Hua Zhao;Key Laboratory of Medicinal Chemistry for Natural Resource Education Ministry, Yunnan University;School of Chemical Science and Technology Pharmacy, Yunnan University;
Coordination polymers (CPs) which can generate solid-state[2+2]cycloaddition reactions have got extensive research as a kind of optical and electronic material in recent years,but fluorescent sensing properties of these CPs are still rare in literature.Therefore,a novel 2D photoreactive CP[Cd_2(bpe)_2(cpa)_2]_n (1) undergoes[2+2]cycloaddition reactions to yield corresponding photoproduct[Cd_2(rctt-tpcb)(cpa)_2]_n (1a) accompanied by single-crystal to single-crystal (SCSC) structural transformation (Fig.1).Furthermore,the fluoresence explorations showed that 1 and1a are dual detection fluorosensors for Fe~(3+)and Cr_2O_7~(2-)ions with high sensitivity,selectivity,and anti-jamming capability (Fig.1).
Wei Chen
Min Ji
Jian Wu
Yong Wang
Jing Zhou
Rong-Rong Zhu
Hong Lu
Huai-Jin Guan
AIM:To investigate the clinical efficacy and safety of femtosecond laser-assisted steepest-meridian clear corneal incisions for correcting preexisting corneal astigmatism performed at the time of cataract surgery.METHODS:This prospective case series study comprised consecutive age-related cataract patients with corneal regular astigmatism(range:+0.75 to+2.50 D)who had femtosecond laser-assisted steepest-meridian clear corneal incisions(single or paired).Corneal astigmatism was performed with the Pentacam preoperatively and 3 mo postoperatively.Total corneal astigmatism and steepestmeridian measured in the 3-mm central zone were used to guide the location,size and number of clear corneal incision.The vector analysis of astigmatic change was performed using the Alpins method.RESULTS:Totally 138 eyes of 138 patients were included.The mean preoperative corneal astigmatism was 1.31±0.41 D,and was significantly reduced to 0.69±0.34 D(equivalent to difference vector)after surgery(P<0.01).The surgically-induced astigmatism was 1.02±0.54 D.The correction index(ratio of target induced astigmatism and surgically-induced astigmatism:0.72±0.36)as well as the magnitude of error(difference between surgically-induced astigmatism and target induced astigmatism:-0.29±0.51)represented a slight under correction.For angle of error,the arithmetic mean was 1.11±13.70,indicating no significant systematic alignment errors.CONCLUSION:Femtosecond-assisted steepest-meridian clear corneal incision is a fast,customizable,adjustable,precise,and safe technique for the reduction of low to moderate corneal astigmatism during cataract surgery.
Wei Chen
Min Ji
Jian Wu
Yong Wang
Jing Zhou
Rong-Rong Zhu
Hong Lu
Huai-Jin Guan
AIM: To investigate the clinical efficacy and safety of femtosecond laser-assisted steepest-meridian clear corneal incisions for correcting preexisting corneal astigmatism performed at the time of cataract surgery.METHODS: This prospective case series study comprised consecutive age-related cataract patients with corneal regular astigmatism（range: +0.75 to +2.50 D） who had femtosecond laser-assisted steepest-meridian clear corneal incisions（single or paired）. Corneal astigmatism was performed with the Pentacam preoperatively and 3 mo postoperatively. Total corneal astigmatism and steepestmeridian measured in the 3-mm central zone were used to guide the location, size and number of clear corneal incision. The vector analysis of astigmatic change was performed using the Alpins method.RESULTS: Totally 138 eyes of 138 patients were included. The mean preoperative corneal astigmatism was 1.31±0.41 D, and was significantly reduced to 0.69±0.34 D（equivalent to difference vector） after surgery（P<；0.01）. The surgically-induced astigmatism was 1.02±0.54 D. The correction index（ratio of target induced astigmatism and surgically-induced astigmatism: 0.72±0.36） as well as the magnitude of error（difference between surgically-induced astigmatism and target induced astigmatism:-0.29±0.51） represented a slight under correction. For angle of error, the arithmetic mean was 1.11±13.70, indicating no significant systematic alignment errors. CONCLUSION: Femtosecond-assisted steepest-meridian clear corneal incision is a fast, customizable, adjustable, precise, and safe technique for the reduction of low to moderate corneal astigmatism during cataract surgery.
Kimberly Mann
Jon Royce
Christina Carbrello
Robert Smith
Rong-Rong Zhu
Yuanchun Zeng
Anne Leahy
Mary Priest
Ven Raman
Jayant S Nick Harrington
Scott Bliss
William Bryant
Nhung Nguyen
Soleil Le
Danielle DeCesaro
Jeremy Perreault
Philip Goddard
Joe Orlando
Kevin Rautio
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