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Now showing items 17 - 32 of 10209

  • A Survey of DDX21 Activity During Rev/RRE Complex Formation

    Hammond, John A.   Zhou, Li   Lamichhane, Rajan   Chu, Hui-Yi   Millar, David P.   Gerace, Larry   Williamson, James R.  

    HIV-1 requires a specialized nuclear export pathway to transport unspliced and partially spliced viral transcripts to the cytoplasm. Central to this pathway is the viral protein Rev, which binds to the Rev response element in stem IIB located on unspliced viral transcripts and subsequently oligomerizes in a cooperative manner. Previous work identified a number of cellular DEAD-box helicases as in vivo binding partners of Rev, and siRNA experiments indicated a functional role for many in the HIV replication cycle. Two DEAD-box proteins, DDX1 and DDX3, had previously been shown to play a role in HIV pathogenesis. In this study, another protein identified in that screen, DDX21, is tested for protein and RNA binding and subsequent enzymatic activities in the context of the Rev/RRE pathway. We found that DDX21 can bind to the RRE with high affinity, and this binding stimulates ATPase activity with an enzymatic efficiency similar to DDX1. Furthermore, DDX21 is both an ATP-dependent and ATP-independent helicase, and both ATPase and ATP-dependent helicase activities are inhibited by Rev in a dose-dependent manner, although ATP-independent helicase activity is not. A conserved binding interaction between DDX protein's DEAD domain and Rev was identified, with Rev's nuclear diffusion inhibitory signal motif playing a significant role in binding. Finally, DDX21 was shown to enhance Rev binding to the RRE in a manner similar to that previously described for DDX1, although DDX3 does not. These data indicate that DDX1 and DDX21 have similar biochemical activities with regard to the Rev/RRE system, while DDX3 differs. (C) 2017 Elsevier Ltd. All rights reserved.
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  • Studying grant decision-making: a linguistic analysis of review reports

    van den Besselaar Peter   Sandstr?m Ulf   Schiffbaenker Hélène  

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  • Applying for Grant Funding as a Counseling Psychologist

    Burrow-Sánchez, Jason J.; Martin, Jessica L.; Imel, Zac E.  

    Counseling psychologists can benefit from grant funding because it can support their research and training of doctoral students. Now more than ever, universities across the nation are encouraging faculty to seek funding from external sources. This reality creates an opportunity for counseling psychologists to develop grant writing skills and take their research to new levels by obtaining funding to support larger scale studies or clinical interventions. We seek to provide a resource for counseling psychologists and doctoral students that provides guidance and tips for successfully seeking and applying for grants that fit areas of research common in our field. Topics covered in this article include how to get involved in the grant writing process, obtaining mentorship, crafting a successful application, and understanding the outcome of a grant review. Grant funding is an attainable goal for counseling psychologists at all stages of their training and career.
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  • The Role of a 1994 Land Grant College

    Halvorson   Gary A.  

    Tribal colleges provide educational opportunities to many Native American people, who otherwise would not be able to attend college. A strong collaboration with a tribal college takes into account the needs and input of the Native Community and does so in a culture-centered way. Discussions with a collaborating tribal college should begin early in the grant writing process. Tribal colleges can make significant contributions to the research effort. These contributions include their own research capabilities, a great cultural experience for everyone involved, and students who will continue their education as a result of their experience with the grant.
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  • [Phys. Rev. A\r 96\r , 022516 (2017)]

    Zaitsevskii, A.   Mosyagin, N. S.   Stolyarov, A. V.   Eliav, E.  

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  • The ABC of writing a grant proposal

    Seeman   E.  

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  • Include a Powerful Evaluation Section in Grant Proposals

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  • [Phys. Rev. A 90 , 052502 (2014)]

    Collister, R.; Gwinner, G.; Tandecki, M.; Behr, J. A.; Pearson, M. R.; Zhang, J.; Orozco, L. A.; Aubin, S.; Gomez, E.  

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  • Include a Powerful Evaluation Section in Grant Proposals

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  • Applying for Grant Funding as a Counseling Psychologist

    Burrow-Sánchez, Jason J.   Martin, Jessica L.   Imel, Zac E.  

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  • [Phys. Rev. A 91 , 031404(R) (2015)]

    Hassouneh, O.; Law, S. M. K.; Shearer, S. F. C.; Brown, A. C.; van der Hart, H. W.  

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  • Using a BAOMS Travel Grant with The Northern Cleft Foundation

    Wicks, Catherine  

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  • On establishing a grant pre-review process for new investigators

    Rutkove   Seward B.  

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  • A Remarkable Legacy: The Wayne F. Placek Fund Grant Program

    Kimmel, Douglas C.   Morin, Stephen F.   Straus, Elisabeth R.  

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  • Young GI Angle: How to apply for a grant

    Bokun, Tomislav; van den Brink, Gijs R.  

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  • Young GI Angle: How to apply for a grant

    Bokun, Tomislav; van den Brink, Gijs R.  

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