The invention provides a nanoparticle (NP)-based detection system for bacteria that is both rapid and sensitive for bacterial analytes in water. The system reports its results colorimetrically, in the form of an easily observable color change, allowing analysis to be done visually by non-scientifically trained personnel.
Creran, Brian
Li, Xiaoning
Duncan, Bradley
Kim, Chang Soo
Moyano, Daniel F
Rotello, Vincent M
Low-cost diagnostics for drinking water contamination have the potential to save millions of lives. We report a method that uses inkjet printing to copattern an enzyme-nanoparticle sensor and substrate on a paper-based test strip for rapid detection of bacteria. A colorimetric response is generated on the paper substrate that allows visual detection of contamination without the need for expensive instrumentation. These strips demonstrate a viable nanomanufacturing strategy for low-cost bacterial detection. =20
Creran, Brian
Li, Xiaoning
Duncan, Bradley
Kim, Chang Soo
Moyano, Daniel F.
Rotello, Vincent M.
Low-cost diagnostics for drinking water contamination have the potential to save millions of lives. We report a method that uses inkjet printing to copattern an enzymenanoparticle sensor and substrate on a paper-based test strip for rapid detection of bacteria. A colorimetric response is generated on the paper substrate that allows visual detection of contamination without the need for expensive instrumentation. These strips demonstrate a viable nanomanufacturing strategy for low-cost bacterial detection.
Jeong, Youngdo
Chen, Yu-Cheng
Turksoy, Merve K
Rana, Subinoy
Tonga, Gulen Yesilbag
Creran, Brian
Sanyal, Amitav
Crosby, Alfred J
Rotello, Vincent M
The elastic modulus of an ultrathin nanoparticle (NP) monolayer film is tuned by modulating the binding strength between the NPs on a molecular level. NP monolayer films constructed by crosslinking NPs of different binding affinities are fabricated at oil/water interfaces. By inducing buckling patterns on these films, the correlation between the binding affinity of the NPs and the elastic modulus is investigated. =C2=A9 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Fitzpatrick, Brian
Creran, Brian
Cooke, Graeme
Rotello, Vincent M.
The synthesis of flavin-containing amphiphilic block copolymers using atom transfer radical polymerisation (ATRP) is described. In these systems, a flavin moiety is engineered into the ATRP initiator unit as an environmental probe and is subsequently used to produce three block copolymers featuring a hydrophobic methacrylate block and a DMAEM block. The dimethylamino unit of the latter is converted to a trimethylammonium unit to afford three amphiphilic block copolymers. Rheological measurements show that these materials form gels in dilute aqueous solution that can be disrupted upon irradiation with ultrasound. The disruption of the gel can be conveniently monitored by exploiting the solvatochromic nature of the S0S2 absorption in the UV-vis spectrum in response to its changing environment.
Yu, Xi
Pham, Jonathan T.
Subramani, Chandramouleeswaran
Creran, Brian
Yeh, Yi-Cheun
Du, Kan
Patra, Debabrata
Miranda, Oscar R.
Crosby, Alfred J.
Rotello, Vincent M.
Park, Myoung-Hwan
Agasti, Sarit S.
Creran, Brian
Kim, Chaekyu
Rotello, Vincent M.
A dendrimer-nanoparticle hybrid scaffold based on robust dithiocarbamate formation provides a controlled drug delivery system. These composite films are nontoxic and can incorporate a variety of guests, providing sustained drug release over multiple uses. The system is highly modular: the release process can be easily tuned by altering the dendrimer generation and the size of the AuNPs, generating a versatile delivery system.
Mumtaz, Shazia
Wang, Li-Sheng
Hussain, Syed Zajif
Abdullah, Muhammad
Huma, Zille
Iqbal, Zafar
Creran, Brian
Rotello, Vincent M
Hussain, Irshad
We report a simple and economical colorimetric bacterial sensing strategy with catalytic amplification using dopamine-capped iron oxide (Dop-Fe3O4) nanoparticles. These nanoparticles catalyse the oxidation of a chromogenic substrate in the presence of H2O2 into a green colored product. The catalytic activity of the nanoparticles is inhibited in the presence of bacteria, providing naked eye detection of bacteria at 104 cfu mL-1 and by spectrophotometric detection down to 102 cfu mL-1.=20
Miranda, Oscar R.
Creran, Brian
Rotello, Vincent M.
Nanoparticle-based arrays have been used to distinguish a wide range of biomolecular targets through pattern recognition. In this report, we highlight new 'chemical nose' methodologies that use nanoparticle systems to provide high sensitivity sensing of biomolecular targets, including fluorescent polymer/gold nanoparticle complexes that can discriminate between different bioanalytes including proteins, bacteria, and mammalian cells as well as dye-based micellar systems for the detection of clinically important metalloproteins and nonmetalloproteins.
Creran, Brian
Yan, Bo
Moyano, Daniel F.
Gilbert, Michael M.
Vachet, Richard W.
Rotello, Vincent M.
Patterns created by the inkjet printing of functionalized gold nanoparticles (NPs) can be selectively detected by laser desorption/ionization imaging mass spectrometry (LDI-IMS). These patterns can only be visualized by mass, providing a robust yet tunable system for potential anti-counterfeiting applications.
Marsico, Alyssa L M
Creran, Brian
Duncan, Bradley
Elci, S Gokhan
Jiang, Ying
Onasch, Timothy B
Wormhoudt, Joda
Rotello, Vincent M
Vachet, Richard W
Effective detection of low molecular weight compounds in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) is often hindered by matrix interferences in the low m/z region of the mass spectrum. Here, we show that monolayer-protected gold nanoparticles (AuNPs) can serve as alternate matrices for the very sensitive detection of low molecular weight compounds such as amino acids. Amino acids can be detected at low fmol levels with minimal interferences by properly choosing the AuNP deposition method, density, size, and monolayer surface chemistry. By inkjet-printing AuNPs at various densities, we find that AuNP clusters are essential for obtaining the greatest sensitivity. Graphical Abstract =E1=85=9F. =20
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