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Now showing items 1 - 6 of 6

  • Risk Factors and Management of Urinary Tract Infections in Children Aged 3 Months to 2 Years

    Chi-Hui Cheng  

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  • Albuminuria in Childhood is a Risk Factor for Chronic Kidney Disease and End-Stage Renal Disease

    Chi-Hui Cheng  

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  • Is Acute Lobar Nephronia the Midpoint in the Spectrum of Upper Urinary Tract Infections between Acute Pyelonephritis and Renal Abscess?

    Chi-Hui Cheng   Yong-Kwei Tsau   Tzou-Yien Lin  

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  • Kidney Volume and Plasma Hepatocyte Growth Factor–Transforming Growth Factor β1 Ratio Among Children With Biliary Atresia Before and After Liver Transplantation: The Reversibility of Nephromegaly

    Chi-Hui Cheng   Yong-Kwei Tsau   I.-Jung Tsai  

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  • Sensing performance of fibronectin-functionalized Au-EGFET on the detection of <em>S. epidermidis</em> biofilm and 16S rRNA of infection-related bacteria in peritoneal dialysis

    Agnes Purwidyantri   Hsin-Chih Lai   Sheng-Hui Tsai   Ji-Dung Luo   Chiuan-Chian Chiou   Ya-Chung Tian   Chi-Hui Cheng   Yi-Ting Lin   Chao-Sung Lai  

    Abstract This study proposed novel methods for Staphylococcus epidermidis biofilm and 16S rRNA of infection-related bacteria detection. S. epidermidis biofilm was attached onto self assembled monolayers (SAMs) coated Au substrate of an extended-gate field-effect-transistor (EGFET) sensor with immobilized extracellular matrix protein, fibronectin. Biofilm mostly composed of positively charged polysaccharide intercellular adhesin (PIA) significantly contributed threshold voltage ( V TH ) shift from −6.67 to −94 mV for biofilm yielded by 3.8 × 10 6 to 3.8 × 10 8 CFU/ml S. epidermidis and obtained limit of detection (LOD) of 9 × 10 5 CFU/ml S. epidermidis . SAMs-Au surface functionalization and its properties after fibronectin immobilization showed an optimal surface roughness from atomic force microscope (AFM) view. The presence of biofilm on the surface was validated by (scanning electron microscope) SEM screening after 1 h incubation at 37 °C. Cellular response to antibiotic treatment against biofilm achieved as contaminated dialysate was treated with and without Vancomycin. The hybridization of a highly conserved gene, 16S rRNA of S. epidermidis and Staphylococcus aureus , the most leading pathogenic bacteria in peritoneal dialysis patients, was also successfully recorded. Findings suggested that Au-EGFET is a promising sensing biochip for detection of bacterial biofilm and determination of species involved in the infection.
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  • Genetic Polymorphisms in Toll-Like Receptors among Pediatric Patients with Renal Parenchymal Infections of Different Clinical Severities

    Chi-Hui Cheng   Yun-Shien Lee   Chee-Jen Chang   Tzou-Yien Lin  

    BACKGROUND Although several studies have suggested single gene defects or variations in the genes associated with host immune response could confer differences in susceptibility to urinary pathogen invasion, no studies have examined the genetic polymorphisms in various toll-like receptors (TLRs) that activate innate immune responses in pediatric renal parenchymal infections of different clinical severities, namely acute pyelonephritis and the clinically more severe disease, acute lobar nephronia. METHODOLOGY Patients who fulfilled the diagnostic criteria for acute pyelonephritis (APN) and acute lobar nephronia (ALN) without underlying diseases or structural anomalies, except for vesicoureteral reflux (VUR), were enrolled. Genotyping of the single nucleotide polymorphisms (SNPs) in the genes encoding TLR-1, TLR-2, TLR-4, TLR-5, and TLR-6 was performed by matrix-assisted laser desorption/ionization time-of-flight-based mini-sequencing analysis. PRINCIPAL FINDINGS A total of 16 SNPs were selected for genotyping. Analysis of 96 normal and 48 patients' samples revealed that only four SNPs had heterozygosity rates >0.01. These SNPs were selected for further investigation. Hardy-Weinberg equilibrium was satisfied for the observed genotype frequencies. Statistically significant differences in the genotype frequency of TLR-2 (rs3804100, T1350C) between controls and ALN or (APN;ALN) combined group were identified using the recessive model with the correction for multiple-SNP testing. Further genotype pattern frequency analysis in TLR-2 SNPs (rs3804099 and rs3804100) showed significantly reduced occurrence of the rare allele homozygote (CC;CC) in the no-VUR subgroup of APN and ALN cases. CONCLUSIONS As the inflammatory responses in ALN patients are more severe than those in APN patients (higher CRP levels, longer duration of fever after antibiotic treatment), these findings suggest that the genetic variant in TLR-2 (rs3804100, T1350C) may protect the host from severe urinary tract infections as ALN.
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