PREPARATION METHOD FOR HUMAN BREAST MILK STEM CELLS AND APPLICATION THEREOF
Abstract(summary):
A preparation method for human breast milk stem cells and an application thereof. The preparation method comprises the following steps: collecting breast milk; separating human breast milk cells to obtained purified breast milk cell suspension; transferring the purified breast milk cell suspension into a culture plate coated with gelatin having a volume percentage of 0.01-0.05%, putting the culture plate into a CO2 incubator for culturing, changing liquid once every day, separating individual cells on the sixth day for cloning and inoculating the cells into a new ultralow-adherency culture plate to promote clone formation; when the cells are fused to 80% or above, adding trypsin, performing digestion at 37°C for 5 min, ending the digestion, collecting cell sediments, washing and centrifugating the cells, resuspending the cells with a fresh cell culture liquid, and perform passage according to the ratio of 1:3. The technical solution is easy to operate, needs no co-culture of feeder cells, and can better maintain the undifferentiated state of the human breast milk stem cells and obtain a larger number of human breast milk stem cells.
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