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METHOD FOR QUANTIFYING NUCLEIC ACID USING MULTIPLE COMPETITOR NUCLEIC ACIDS

Abstract(summary):

A method of determining the amount of target nucleic acid in a sample which comprises the steps of: a) adding to said sample a known amount of at least two different competitor nucleic acids at different concentrations, which have at least a portion of the sequence in common with the target nucleic acid, said common sequence comprising a binding site for a complementary primer sequence, b) co-amplifying the target nucleic acid and competitor nucleic acids in the sample by an in vitro amplification reaction using at least one primer, wherein at least one of said primers comprises a region complementary to said common sequence and the amplification products carry a label or means for attaching a label, c) separation of the amplification products, d) assessing the amount of label associated with the amplification products and e) comparison of the amount of label associated with each of the amplified target nucleic acid and amplified competitor nucleic acids to assess the amount of target nucleic acid in said sample, a method of diagnosis using the assay and kits for performing the same.


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