Induced neuronal activity does not attenuate amyloid beta-induced synaptic loss in vitro.
Journal:
Neuropsychopharmacology reports
Issue Date:
2019
Abstract(summary):
AIM: The accumulation of amyloid beta (Abeta) is one of the characteristics of Alzheimer's disease. The excessive accumulation of Abeta has been suggested to result in a decrease in the number of synapses. Although the number of synapses is generally modulated by neuronal activity, whether neuronal activity affects Abeta-induced synapse loss remains unknown. Therefore, we addressed this question using a primary culture of hippocampal neurons.; METHOD: The neuronal activity of cultured hippocampal neurons from mouse pups was increased using the chemogenetic technique designer receptors exclusively activated by designer drugs (DREADD). The cultured neurons were treated with Abeta, and synapse density was assessed by immunocytochemistry.; RESULTS: Abeta decreased the synapse density probably by decreasing postsynapse. On the other hand, enhanced neuronal activity did not affect the synapse density significantly. However, there was a trend that enhanced neuronal activity increased especially presynapse density.; CONCLUSION: We found that enhanced neuronal activity did not affect Abeta-induced synapse loss in vitro. =C2=A9 2019 The Authors. Neuropsychopharmacology Reports published by John Wiley & Sons Australia, Ltd on behalf of The Japanese Society of NeuropsychoPharmacology.
Page:
306---311
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