Creat membership Creat membership
Sign in

Forgot password?

Confirm
  • Forgot password?
    Sign Up
  • Confirm
    Sign In
Creat membership Creat membership
Sign in

Forgot password?

Confirm
  • Forgot password?
    Sign Up
  • Confirm
    Sign In
Collection
For ¥0.57 per day, unlimited downloads CREATE MEMBERSHIP Download

toTop

If you have any feedback, Please follow the official account to submit feedback.

Turn on your phone and scan

home > search >

A simple visual method for DNA detection based on the formation of gold nanoparticles

Author:
Yuan Liu  Taotao Li  Chuxuan Ling  Zunliang Wang  Lian Jin  Yongxiang Zhao  Zhu Chen  Song Li  Yan Deng  Nongyue He    


Journal:
Chinese Chemical Letters


Issue Date:
2019


Abstract(summary):

A simple visual method for DNA detection during the formation of gold nanoparticles(AuNPs) was developed based on different electrostatic properties of single strand DNA(ssDNA) and double strand DNA(dsDNA).Since the ssDNA is easy to bind to AuNPs due to its exposed bases which could prevent saltinduced aggregation of AuNPs.The dsDNA always present negative charge because its negatively charged phosphate backbone is exposed.In this case,the dsDNA could disturb the adsorption between dsDNA and AuNPs and result in non-aggregation of AuNPs.After hybridization,chloroauric acid and ascorbic acid were added to the mixture solution,and the solution changed to red immediately and turned to purple in10 min in the present of target DNA.TEM results confirmed that the change of color stemed from aggregation of AuNPs.In order to obtain accurate results by naked eye,the DNA detection assay should be conducted under pH 7.0.


Page:
388-391


VIEW PDF

The preview is over

If you wish to continue, please create your membership or download this.

Create Membership

Similar Literature

Submit Feedback

This function is a member function, members do not limit the number of downloads