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Development of a fast DNA extraction method for sea food and marine species identification

Author:
Tagliavia, Marcello  Nicosia, Aldo  Salamone, Monica  Biondo, Girolama  Bennici, Carmelo Daniele  Mazzola, Salvatore  Cuttitta, Angela  


Journal:
FOOD CHEMISTRY


Issue Date:
2016


Abstract(summary):

The authentication of food components is one of the key issues in food safety. Similarly taxonomy, population and conservation genetics as well as food web structure analysis, also rely on genetic analyses including the DNA barcoding technology. In this scenario we developed a fast DNA extraction method without any purification step from fresh and processed seafood, suitable for any PCR analysis. The protocol allows the fast DNA amplification from any sample, including fresh, stored and processed seafood and from any waste of industrial fish processing, independently of the sample storage method. Therefore, this procedure is particularly suitable for the fast processing of samples and to carry out investigations for the authentication of seafood by means of DNA analysis. (C) 2016 Elsevier Ltd. All rights reserved.


Page:
375---378


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