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The reaction mechanism of retaining glycosyltransferases

Author:
Ardevol, Albert  Iglesias-Fernandez, Javier  Rojas-Cervellera, Victor  Rovira, Carme  


Journal:
BIOCHEMICAL SOCIETY TRANSACTIONS


Issue Date:
2016


Abstract(summary):

The catalytic mechanism of retaining glycosyltransferases (ret-GTs) remains a controversial issue in glycobiology. By analogy to the well-established mechanism of retaining glycosidases, it was first suggested that ret-GTs follow a double-displacement mechanism. However, only family 6 GTs exhibit a putative nucleophile protein residue properly located in the active site to participate in catalysis, prompting some authors to suggest an unusual single-displacement mechanism [named as front-face or S(N)i (substitution nucleophilic internal)-like]. This mechanism has now received strong support, from both experiment and theory, for several GT families except family 6, for which a double-displacement reaction is predicted. In the last few years, we have uncovered the molecular mechanisms of several retaining GTs by means of quantum mechanics/molecular mechanics (QM/MM) metadynamics simulations, which we overview in the present work.


Page:
51---60


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