Assembly of an early-matured japonica (Geng) rice genome,Suijing18,based on PacBio and Illumina sequencing

Author:

Nie, Shou-Jun  Liu, Yu-Qiang  Wang, Chun-Chao  Gao, Shi-Wei  Xu, Tian-Tian  Liu, Qing  Chang, Hui-Lin  Chen, Yu-Bao  Yan, Peng-Cheng  Peng, Wei  Zheng, Tian-Qing  Xu, Jian-Long  Li, Zhi-Kang  

Journal:

SCIENTIFIC DATA

Issue Date:

2017

Abstract(summary):

The early-matured japonica (Geng) rice variety, Suijing18 (SJ18), carries multiple elite traits including durable blast resistance, good grain quality, and high yield. Using PacBio SMRT technology, we produced over 25 Gb of long-read sequencing raw data from SJ18 with a coverage of 62 x. Using Illumina paired-end whole-genome shotgun sequencing technology, we generated 59 Gb of short-read sequencing data from SJ18 (23.6 Gb from a 200 bp library with a coverage of 59 x and 35.4 Gb from an 800 bp library with a coverage of 88 x). With these data, we assembled a single SJ18 genome and then generated a set of annotation data. These data sets can be used to test new programs for variation deep mining, and will provide new insights into the genome structure, function, and evolution of SJ18, and will provide essential support for biological research in general.