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The effect of solution pH on the structural stability of magnetoferritin

Author:
L. Balejčíková  V.M. Garamus  M.V. Avdeev  V.I. Petrenko  L. Almásy  P. Kopčanský  


Journal:
Colloids and Surfaces B: Biointerfaces


Issue Date:
2017


Abstract(summary):

Graphical abstract Highlights • The highest stability of magnetoferritin was shown for the region of pH = 7-9. • The “full protein shell” was confirmed in supernatant at pH ∼ 4. • The lowest stability of magnetoferritin was observed at low pH below 6. Abstract The structural stability of magnetoferritin, a synthetic analogue of ferritin, at various pH levels is assessed here. The structural and electrical properties of the complexes were determined by small-angle X-ray scattering (SAXS), dynamic light scattering (DLS) and zeta potential measurements. At pH 3 − 6 a reduction of electrostatic repulsion on the suspended colloids resulted in aggregation and sedimentation of magnetoferritin. At neutral to slightly alkaline conditions (pH 7–9) the magnetoferritin structure was stable for lower iron loadings. Higher solution pH 10–12 induced destabilization of the protein structure and dissociation of subunits. Increasing the loading factor in the MFer complex leads to decrease of the stability versus pH changes.


Page:
375-375


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