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Plasmonics-based SERS nanobiosensor for homogeneous nucleic acid detection

Author:
Wang, Hsin-Neng   Fales, Andrew M.   Vo-Dinh, Tuan  


Journal:
Nanomedicine: Nanotechnology, Biology and Medicine


Issue Date:
2015


Abstract(summary):

Abstract

Developing a simple and efficient nucleic acid detection technology is essential for clinical diagnostics. Here, we describe a new conceptually simple and selective “turn on” plasmonics-based nanobiosensor, which integrates non-enzymatic DNA strand-displacement hybridization for specific nucleic acid target identification with surface-enhanced Raman scattering (SERS) detection. This SERS nanobiosensor is a target label-free, and rapid nanoparticle-based biosensing system using a homogeneous assay format that offers a simple and efficient tool for nucleic acid diagnostics. Our results showed that the nanobiosensor provided a limit of detection of ~ 0.1 nM (200 amol) in the current bioassay system, and exhibited high specificity for single nucleotide mismatch discrimination.

From the Clinical Editor

Surface-enhanced Raman scattering (SERS) is a sensitive technique that enhances Raman scattering by molecules adsorbed on rough metal surfaces. The enhancement means that the technique may even detect single molecules. In this article, the authors describe a simple and efficient nucleic acid detection technology using SERS, with "OFF-to-ON" signal switch upon nucleic acid target identification and capture, which provides high sensitivity and specificity for single nucleotide mismatch discrimination. This new technology will be most welcomed in clinical diagnostics.

Graphical Abstract

A new “turn on” plasmonics-based nanobiosensor for nucleic acid detection has been developed. This nanobiosensor integrating non-enzymatic DNA strand-displacement hybridization for specific target identification with surface-enhanced Raman scattering (SERS) detection is a target label-free and homogeneous nanoparticle-based biosensing system.



Page:
811-814


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