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Developing a simple and efficient nucleic acid detection technology is essential for clinical diagnostics. Here, we describe a new conceptually simple and selective “turn on” plasmonics-based nanobiosensor, which integrates non-enzymatic DNA strand-displacement hybridization for specific nucleic acid target identification with surface-enhanced Raman scattering (SERS) detection. This SERS nanobiosensor is a target label-free, and rapid nanoparticle-based biosensing system using a homogeneous assay format that offers a simple and efficient tool for nucleic acid diagnostics. Our results showed that the nanobiosensor provided a limit of detection of ~ 0.1 nM (200 amol) in the current bioassay system, and exhibited high specificity for single nucleotide mismatch discrimination.
Surface-enhanced Raman scattering (SERS) is a sensitive technique that enhances Raman scattering by molecules adsorbed on rough metal surfaces. The enhancement means that the technique may even detect single molecules. In this article, the authors describe a simple and efficient nucleic acid detection technology using SERS, with "OFF-to-ON" signal switch upon nucleic acid target identification and capture, which provides high sensitivity and specificity for single nucleotide mismatch discrimination. This new technology will be most welcomed in clinical diagnostics.
A new “turn on” plasmonics-based nanobiosensor for nucleic acid detection has been developed. This nanobiosensor integrating non-enzymatic DNA strand-displacement hybridization for specific target identification with surface-enhanced Raman scattering (SERS) detection is a target label-free and homogeneous nanoparticle-based biosensing system.
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