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Kinetic and thermodynamic characterization of adenylyl cyclase from Euglena gracilis

Author:
Ricardo Jasso-Chávez   Alicia Vega-Segura   Mohammed El-Hafidi   Rafael Moreno-Sánchez   M Eugenia Torres-Márquez  


Journal:
Archives of Biochemistry and Biophysics


Issue Date:
2002


Abstract(summary):

Some kinetic and thermodynamic properties of the plasma membrane adenylyl cyclase (AC) from the protist Euglena gracilis were examined. The AC kinetics for Mg-ATP was hyperbolic with a Km value of 0.33-0.43 mM, whereas the inhibition exerted by 2', 5'-dideoxyadenosine was of the mixed type with a Ki of 80-147 muM. The Vm value (0.9 or 1.8 nmol (mg protein)-1 min-1) changed, depending upon the carbon source in the growth medium (lactic acid or glutamate plus malate). Lactic acid membrane AC was slightly more thermolabile (from 28 to 40degreeC) and showed higher activation energy (range 15-25degreeC). With lactate, the total and saturated fatty acid percentage content in the plasma membrane was significantly greater than with glutamate plus malate, whereas the percentage content of polyunsaturated (n-3) fatty acids was lower. The data suggest that the fatty acid composition, as changed by the carbon source in the growth medium, may modulate the AC activity in Euglena.


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