Creat membership Creat membership
Sign in

Forgot password?

Confirm
  • Forgot password?
    Sign Up
  • Confirm
    Sign In
Creat membership Creat membership
Sign in

Forgot password?

Confirm
  • Forgot password?
    Sign Up
  • Confirm
    Sign In
Collection
For ¥0.57 per day, unlimited downloads CREATE MEMBERSHIP Download

toTop

If you have any feedback, Please follow the official account to submit feedback.

Turn on your phone and scan

home > search >

Cloning, characterization and expression analysis of a CXCL10-like chemokine from turbot (Scophthalmus maximus)

Author:
Yang Liu   Song-Lin Chen   Liang Meng   Yu-Xi Zhang  


Journal:
Aquaculture


Issue Date:
2007


Abstract(summary):

Chemokines represent a superfamily of chemotactic cytokines playing an important role in leukocyte chemotaxis. We have constructed a turbot spleen cDNA library that has over 2.2 × 106 primary clones. Its average insert size was estimated to about 700–2000 bp by PCR of 40 randomly picked clones. A novel turbot CXC chemokine was screened from this library. Both 5′-RACE and 3′-RACE were carried out in order to obtain the complete cDNA, which contains a 79 bp 5′ UTR, a 372 bp open reading frame encoding 123 amino acids and a 227 bp 3′ UTR. Four exons and three introns were identified in the turbot CXC chemokine gene. The three introns were 591 bp, 86 bp and 125 bp, respectively. Both BLASTP similarity comparisons and Phylogenetic analysis showed that the turbot CXC chemokine was closer to CXCL10. Interestingly, the highest expression levels of the turbot CXC chemokine were detected at 12 h in liver and spleen after challenge with V. anguillarum and at 5 h in head kidney. The expression in liver decreased gradually after 12 h and returned the level of background after 72 h. In spleen, the expression kept high levels till 24 h and then became faint consistent with background. The expression of the CXC chemokine was not detected in normal tissues of turbot, which suggested it only was induced when challenged with V. anguillarum. These results indicated that the turbot CXC chemokine played an important role in turbot immune response.


Page:
0-207


VIEW PDF

The preview is over

If you wish to continue, please create your membership or download this.

Create Membership

Similar Literature

Submit Feedback

This function is a member function, members do not limit the number of downloads