Halodule wrightii is a seagrass widely distributed in tropical areas, particularly in the western Atlantic. We have developed eight microsatellite markers from H. wrightii for use in genetic studies. The number of alleles ranged from 2 to 12 and the observed and expected heterozygosity ranged from 0.080 to 0.88, and from 0.077 to 0.86, respectively. No locus pair showed significant linkage disequilibrium or deviation from Hardy-Weinberg proportions after Bonferroni correction (P < 0.05). These markers should prove useful for elucidating genetic structure, phylogenetic relationships, and ecological processes in this foundational coastal species.
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