Creat membership Creat membership
Sign in

Forgot password?

Confirm
  • Forgot password?
    Sign Up
  • Confirm
    Sign In
Creat membership Creat membership
Sign in

Forgot password?

Confirm
  • Forgot password?
    Sign Up
  • Confirm
    Sign In
Collection
For ¥0.57 per day, unlimited downloads CREATE MEMBERSHIP Download

toTop

If you have any feedback, Please follow the official account to submit feedback.

Turn on your phone and scan

home > search >

Use Pgal agarose minimal plate to screenlacconstitutive mutation

Author:
Mingli Tang   Li Wang   Pingping Zhang   Pei Huang   Lijun Wu  


Journal:
Annals of microbiology


Issue Date:
2009


Abstract(summary):

ThelacI gene has been used as a target gene in various mutation assays in bacteria and transgenic organisms. Pgal minimal plate is commonly used to selectlac constitutive mutation. To simplify the preparation of the Pgal minimal plate, the agarose (1%) was used to replace agar (1.5%) in the Pgal minimal plate, omitting the process of spreading thelac? bacteria on the plate for scavenging the alternative carbon sources from the plate in advance of use. The results showed that the reducing sugar in agarose was extremely lower than that of others, the selective sensitivity and efficiency of the Pgal agarose was higher than those of the Pgal agar and Pgal agar scavenged. This might give the conclusion that Pgal agarose could be used conveniently and efficiently for the screening oflac constitutive mutation.


Page:
179-182


VIEW PDF

The preview is over

If you wish to continue, please create your membership or download this.

Create Membership

Similar Literature

Submit Feedback

This function is a member function, members do not limit the number of downloads