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The effect of collection date and frozen storage on the formation of embryo-like structures and elongating shoots fromexplants from mature Larix decidua and L. x eurolepis

Author:
Bonga  J.M.  


Journal:
Plant Cell, Tissue and Organ Culture


Issue Date:
1997


Abstract(summary):

Branches were collected in the winter and early spring from a 37-year-oldLarix decidua and a L. x eurolepis tree. Part of these were used immediatelyand the rest stored at −5 °C and −10°C for 3 months. Primordial shoot explants were excisedfrom stored and non-stored material and cultured on half-strength Litvaymedium free of growth regulators. Storage at −5 °Cpromoted formation of embryo-like structures which formed shoots withelongated stems (long-shoots). The highest number of these was obtained withmaterial collected shortly before bud break. De-needled stem sections, cutfrom elongating stems in early summer, produced axillary short-shootsinstead of embryo-like structures.



Page:
195-200


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