Genetic analysis of pyriproxyfen resistance in the housefly, Musca domestica L.

Journal:

Applied Entomology and Zoology

Issue Date:

1997

Abstract(summary):

A M. domestica strain highly resistant to pyriproxyfen, a juvenile hormone analogue, was established by laboratory selection of flies collected from the 3rd Yumenoshima dumping-island in Tokyo Bay, Japan, during 1987. The strain developed 4900-foldre%sistance to pyriproxyfen in the 3rd instar larvae by the 17th generation of selection from a level of only 3.8-fold before selection (compared to susceptible strains). The selected population was named YPPF strain. Genetic analysis of pyriproxyfen resistance in YPPF houseflies revealed that resistance to pyriproxyfen in this strain was incompletely dominant and multifactorial. There were at least 4 dominant factors responsible for the resistance, with the most important factor being located on the 2ndchromosome and other factors on the 1st, 3rd and 5th chromosomes. Furthermore, bioassays of synergism showed that the resistance ratio in white pupae of M. domestica was depressed to 29-fold from 400-fold by a cytochrome P450 inhibitor, 2-propynyl 2,3,6-trichlorophenyl ether. These results indicate that P450 monooxygenase plays an important role in M. domestica resistance to pyriproxyfen.

Page:

217-226