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Frozen storage stimulates the formation of embryo-like structures and elongating shoots in explants from mature Larix decidua and L x eurolepsis trees

Author:
Bonga, JM  


Journal:
PLANT CELL TISSUE AND ORGAN CULTURE


Issue Date:
1996


Abstract(summary):

Branches were collected from a Larix decidua and a L. x eurolepis tree, both 36 years old, in mid-winter. These branches were placed in freezers at -5 degrees C, -10 degrees C, and -18 degrees C. Primordial shoot explants were excised after 2 to 9 months of frozen storage. The material remained viable at all three temperatures for at least 9 months. The frozen storage stimulated formation of embryo-like structures that were capable of forming shoots with elongated stems.


Page:
91---101


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