Creat membership Creat membership
Sign in

Forgot password?

Confirm
  • Forgot password?
    Sign Up
  • Confirm
    Sign In
Creat membership Creat membership
Sign in

Forgot password?

Confirm
  • Forgot password?
    Sign Up
  • Confirm
    Sign In
Collection

toTop

If you have any feedback, Please follow the official account to submit feedback.

Turn on your phone and scan

home > search >

Regulation of M-type potassium channels in mammalian sympathetic neurons: Action of intracellular calcium on single channel currents

Author:
Selyanko, AA  Brown, DA  


Journal:
NEUROPHARMACOLOGY


Issue Date:
1996


Abstract(summary):

Currents through single M-type potassium channels were recorded in membrane patches excised from rat superior cervical sympathetic neurons. Application of Ca2+ to the internal face of inside-out patches produce two forms of M-channel inhibition: a slow, all-or-nothing suppression of activity; and a fast block associated with a concentration-dependent shortening of open times compatible with open-channel block. Both forms of block were enhanced by patch depolarization. Neither was replicated or affected by Mg2+, and both could be recorded in the absence of intracellular ATP, implying that they did not involve phosphorylation. Since the block was reversible in the absence of ATP and since alkaline phosphatase did not reduce channel activity, block was unlikely to have resulted from dephosphorylation. In cell-attached patch recordings, M-channel activity increased during exposure of the cell to Ca2+-free solution and was rapidly reduced on applying 2 mM Ca2+ to the extra-patch solution. This suggests that M-channel activity in these neurons may be tonically regulated by variations in resting intracellular [Ca2+]. Copyright (C) 1996 Elsevier Science Ltd


Page:
933---947


Similar Literature

Submit Feedback

This function is a member function, members do not limit the number of downloads