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Now showing items 1 - 16 of 62

  • Analogues of 4H-pyrazolo[1,5-a] benzimidazole compound as PARP inhibitors

    Disclosed is a series of analogs of 4H-pyrazolo[1,5-α]benzimidazole compound as PARP inhibitors. In particular, disclosed in the invention is a compound as shown by formula (I) or a pharmaceutically acceptable salt thereof as a PARP inhibitor.
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  • Role of unusual CD4+CD28?T cells in acute coronary syndrome

    Wenjie Sun   Lihui Zheng   Lijuan Huang  

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  • Construction of an ab initio kinetic model for industrial ethane pyrolysis

    Wenjie Sun   Mark Saeys  

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  • Collagen scaffolds loaded with collagen-binding NGF-β accelerate ulcer healing

    Wenjie Sun   Hang Lin   Bing Chen   Wenxue Zhao   Yannan Zhao   Zhifeng Xiao   Jianwu Dai  

    Abstract Studies have shown that exogenous nerve growth factor (NGF) accelerates ulcer healing, but the inefficient growth factor delivery system limits its clinical application. In this report, we found that the native human NGF-β fused with a collagen-binding domain (CBD) could form a collagen-based NGF targeting delivery system, and the CBD-fused NGF-β could bind to collagen membranes efficiently. Using the rabbit dermal ischemic ulcer model, we have found that this targeting delivery system maintains a higher concentration and stronger bioactivity of NGF-β on the collagen membranes by promoting peripheral nerve growth. Furthermore, it enhances the rate of ulcer healing through accelerating the re-epithelialization of dermal ulcer wounds and the formation of capillary lumens within the newly formed tissue area. Thus, collagen membranes loaded with collagen-targeting human NGF-β accelerate ulcer healing efficiently. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
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  • Activated collagen scaffold materials and their special fused active restoration factors

    Provided are activated collagen scaffold materials as well as their special fused active restoration factors useful for promoting tissue repair, such as bone damage repair or nerve injury repair. The special fused active restoration factors are fusion proteins comprising a collagen-binding domain (CBD) at N-/C-terminus of cytokines, wherein the collagen-binding domain is a polypeptide consisting of 7-27 amino acid residues with a conservative sequence shown in SEQ ID NO:12 at N-terminus.
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  • Activated collagen scaffold materials and their special fused active restoration factors

    Provided are activated collagen scaffold materials as well as their special fused active restoration factors useful for promoting tissue repair, such as bone damage repair or nerve injury repair. The special fused active restoration factors are fusion proteins comprising a collagen-binding domain (CBD) at N-/C-terminus of cytokines, wherein the collagen-binding domain is a polypeptide consisting of 7-27 amino acid residues with a conservative sequence shown in SEQ ID NO:4 at N-terminus.
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  • Anoxic oxidation of arsenite linked to denitrification in sludges and sediments

    Wenjie Sun   Reyes Sierra   Jim A. Field  

    In this study, denitrification linked to the oxidation of arsenite (As(III)) to arsenate (As(V)) was shown to be a widespread microbial activity in anaerobic sludge and sediment samples that were not previously exposed to arsenic contamination. When incubated with 0.5 mM As(III) and 10 mM NO3−, the anoxic oxidation of As(III) commenced within a few days, achieving specific activities of up to 1.24 mmol As(V) formed g−1 volatile suspended solids d−1 due to growth (doubling times of 0.74–1.4 d). The anoxic oxidation of As(III) was partially to completely inhibited by 1.5 and 5 mM As(III), respectively. Inhibition was minimized by adding As(III) adsorbed onto activated aluminum (AA). The oxidation of As(III) was shown to be linked to the complete denitrification of NO3− to N2 by demonstrating a significantly enhanced production of N2 beyond the background endogenous production as a result of adding As(III)–AA to the cultures. The N2 production corresponded closely the expected stoichiometry of the reaction, 2.5 mol As(III) mol−1 N2–N. The oxidation of As(III) linked to the use of common-occurring nitrate as an electron acceptor may be an important missing link in the biogeochemical cycling of arsenic.
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  • Anoxic oxidation of arsenite linked to denitrification in sludges and sediments

    Wenjie Sun   Reyes Sierra   Jim A. Field  

    In this study, denitrification linked to the oxidation of arsenite (As(III)) to arsenate (As(V)) was shown to be a widespread microbial activity in anaerobic sludge and sediment samples that were not previously exposed to arsenic contamination. When incubated with 0.5 mM As(III) and 10 mM NO3−, the anoxic oxidation of As(III) commenced within a few days, achieving specific activities of up to 1.24 mmol As(V) formed g−1 volatile suspended solids d−1 due to growth (doubling times of 0.74–1.4 d). The anoxic oxidation of As(III) was partially to completely inhibited by 1.5 and 5 mM As(III), respectively. Inhibition was minimized by adding As(III) adsorbed onto activated aluminum (AA). The oxidation of As(III) was shown to be linked to the complete denitrification of NO3− to N2 by demonstrating a significantly enhanced production of N2 beyond the background endogenous production as a result of adding As(III)–AA to the cultures. The N2 production corresponded closely the expected stoichiometry of the reaction, 2.5 mol As(III) mol−1 N2–N. The oxidation of As(III) linked to the use of common-occurring nitrate as an electron acceptor may be an important missing link in the biogeochemical cycling of arsenic.
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  • Activated collagen scaffold materials and their special fused active restoration factors

    Provided are activated collagen scaffold materials as well as their special fused active restoration factors useful for promoting tissue repair, such as bone damage repair or nerve injury repair. The special fused active restoration factors are fusion proteins comprising a collagen-binding domain (CBD) at N-/C-terminus of cytokines, wherein the collagen-binding domain is a polypeptide consisting of 7-27 amino acid residues with a conservative sequence shown in SEQ ID NO:1 at N-terminus.
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  • Moderate Alcohol Use, Health Status, and Mortality in a Prospective Chinese Elderly Cohort

    Wenjie Sun   C. Mary Schooling   Wai Man Chan   Kin Sang Ho   Tai Hing Lam   Gabriel M. Leung  

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  • Moderate Alcohol Use, Health Status, and Mortality in a Prospective Chinese Elderly Cohort

    Wenjie Sun   C. Mary Schooling   Wai Man Chan   Kin Sang Ho   Tai Hing Lam   Gabriel M. Leung  

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  • Avoiding Coal–Water Conflicts During the Development of China’s Large Coal-Producing Regions

    Wenjie Sun   Qiang Wu   Donglin Dong   Jian Jiao  

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  • A new approach to ovarian reserve testing

    Wenjie Sun   Barbara J. Stegmann   Melinda Henne   William H. Catherino   James H. Segars  

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  • Anoxic oxidation of arsenite linked to chemolithotrophic denitrification in continuous bioreactors

    Wenjie Sun   Reyes Sierra-Alvarez   Ivann Hsu   Pieter Rowlette   Jim A. Field  

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  • Assessing protein oxidation by inorganic nanoparticles with enzyme-linked immunosorbent assay (ELISA)

    Wenjie Sun   Antonia Luna-Velasco   Reyes Sierra-Alvarez and Jim A. Field  

    Growth in the nanotechnology industry is leading to increased production of engineered nanoparticles (NPs). This has given rise to concerns about the potential adverse and toxic effects to biological system and the environment. An important mechanism of NP toxicity is oxidative stress caused by the formation of reactive oxygen species (ROS) or via direct oxidation of biomolecules. In this study, a protein oxidation assay was developed as an indicator of biomolecule oxidation by NPs. The oxidation of the protein, bovine serum albumin (BSA) was evaluated with an enzyme-linked immunosorbent assay (ELISA) to measure the protein carbonyl derivatives formed from protein oxidation. The results showed that some NPs such as Cu(0), CuO, Mn2O3, and Fe(0) caused oxidation of BSA; whereas, many of the other NPs tested were not reactive or very slowly reactive with BSA. The mechanisms involved in the oxidation of BSA protein by the reactive NPs could be attributed to the combined effects of ROS-dependent and direct protein oxidation mechanisms. The ELISA assay is a promising method for the assessment of protein oxidation by NPs, which can provide insights on NP toxicity mechanisms. Biotechnol. Bioeng. 2013; 110: 694–701.
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  • Promotion of peripheral nerve growth by collagen scaffolds loaded with collagen-targeting human nerve growth factor-β

    Wenjie Sun   Hang Lin   Bing Chen   Wenxue Zhao   Yannan Zhao   Jianwu Dai  

    Abstract Nerve growth factor (NGF) plays a critical role in neuronal development and regeneration. However, the lack of efficient NGF delivery system limits its clinical application. We reported that a peptide deduced from collagenase, TKKTLRT, fused with NGF-β could develop a collagen based NGF targeting delivery system. Our results showed that this peptide could allow fused NGF-β bind to collagen specifically. In addition, we found that the polypeptide could result in a 2.3-fold increase in the expression level and a significant improvement of bioactivity of fused NGF-β. In the in vivo function study, collagen membranes loaded with the collagen binding NGF enhanced the nerve growth. Thus, the targeting wound repair system could be important for the repair of peripheral nerve injury. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007
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