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Now showing items 49 - 64 of 615

  • Targeted Delivery of Tumor Antigens to Activated Dendritic Cells via CD11c Molecules Induces Potent Antitumor Immunity in Mice

    Wei, H.   Wang, S.   Zhang, D.   Hou, S.   Qian, W.   Li, B.   Guo, H.   Kou, G.   He, J.   Wang, H.   Guo, Y.  

    Purpose: CD11c is an antigen receptor predominantly expressed on dendritic cells (DC), to which antigen targeting has been shown to induce robust antigen-specific immune responses. To facilitate targeted delivery of tumor antigens to DCs, we generated fusion proteins consisting of the extracellular domain of human HER or its rat homologue neu, fused to the single-chain fragment variable specific for CD11c (scFv(CD11c)-HER2/neu). Experimental Design: Induction of cellular and humoral immune responses and antitumoral activity of the fusion proteins admixed with DC-activating CpG oligonucleotides (scFV(CD11C)-HER2/neu(CpG)) were tested in transplantable HER2/neu-expressing murine tumor models and in transgenic BALB-neuT mice developing spontaneous neu-driven mammary carcinomas. Results: Vaccination of BALB/c mice with scFv(CD11c)-HER2(CpG) protected mice from subsequent challenge with HER2-positive, but not HER2-negative, murine breast tumor cells, accompanied by induction of strong HER2-specific T-cell and antibody responses. In a therapeutic setting, injection of scFv(CD11c)-HER2(CpG), caused rejection of established HER2-positive tumors. Importantly, antitumoral activity of such a fusion protein vaccine could be reproduced in immuno-tolerant BALB-neuT mice, where scFv(CD11c)-neu(CpG) vaccination significantly protected against a subsequent challenge with neu-expressing murine breast tumor cells and markedly delayed the onset of spontaneous mammary carcinomas. Conclusions: CD11c-targeted protein vaccines for in vivo delivery of tumor antigens to DCs induce potent immune responses and antitumoral activities and provide a rationale for further development of this approach for cancer immunotherapy.
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  • Expression of c-kit Protein and Mutational Status of the c-kit Gene in Osteosarcoma and Their Clinicopathological Significance

    Wei, H.   Zhao, M.   Dong, W.   Yang, Y.   Li, J.  

    We examined c-kit protein expression and mutations of the c-kit gene in 40 human osteosarcoma samples to their relationship with clinicopathology and prognosis of the disease. The expression of c-kit protein was evaluated by immunohistochemistry and single-strand conformational polymorphism was performed to evaluate c-kit gene mutations in exons 11 and 17. Expression of c-kit protein occurred in 25 (62.5%) osteosarcoma samples. Patients with osteosarcomas with higher c-kit protein expression levels were significantly more likely to experience local disease recurrence and had a significantly lower survival time than patients with lower c-kit expression. We found no evidence of mutations in exons 11 or 17. This study suggests that c-kit protein expression might serve as a prognostic marker for osteosarcoma, however exons 11 and 17 might not be suitable targets for osteosarcoma treatments based on suppression of c-kit tyrosine kinase activity.
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  • A Dominant Major Locus in Chromosome 9 of Rice (Oryza sativa L.) Confers Tolerance to 48 ?C High Temperature at Seedling Stage

    Wei, H.   Liu, J.   Wang, Y.   Huang, N.   Zhang, X.   Wang, L.   Zhang, J.   Tu, J.   Zhong, X.  

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  • Duality theorem for a generalized Fermat-Weber problem : Wilfred Kaplan and Wei H. Yang Mathematical Programming Vol. 76, 1997, 285–297

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  • 99/01028 Petrologic indication of oil-generating coal : Wei, H. and Yao, S. Shiyou Kantan Yu Kaifa, 1997, 24, (6), 32–35. (In Chinese)

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  • Prescriptions for Saving China: Selected Writings of Sun Yat-sen.by Julie Lee Wei; Ramon H. Myers; Donald G. Gillin

    Review by: Harold Z. Schiffrin  

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  • Site preference of Re in NiAl and valence band structure of NiAl containing Re: First-principles study and photoelectron spectrum

    Wei, H.   Liang, J. J.   Sun, B. Z.   Zheng, Q.   Sun, X. F.   Peng, P.   Yao, X.   Dargusch, M. S.  

    The site preference of Re in NiAl was studied using first-principles calculations. The calculation of formation energies of the NiAl alloys indicated the site preference of Re on the Ni sites. The valence band structures of the NiAl alloys were investigated by photoelectron spectroscopy. The valence band spectra of the NiAl with Re shifted away from the Fermi energy level so that the Ni d-band centroid moved to a higher energy by 0.25 eV as Re was added. Such a shift could be attributed to the Ni-Re interaction, which was supported by the photoelectron spectroscopy measurement.
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  • A Tragic Beginning: The Taiwan Uprising of February 28, 1947by Lai Tse-han; Ramon H. Myers; Wei Wou

    Review by: Neville Bennett  

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  • A Tragic Beginning: The Taiwan Uprising of February 28, 1947.by Lai Tse-han; Ramon H. Myers; Wei Wou

    Review by: John F. Copper  

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  • A Tragic Beginning: The Taiwan Uprising of February 28, 1947.by Lai Tse-Han; Ramon H. Myers; Wei Wou

    Review by: C. L. Chiou  

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  • A Tragic Beginning: The Taiwan Uprising of February 28, 1947.by Lai Tse-han; Ramon H. Myers; Wei Wou

    Review by: Harry J. Lamley  

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  • A study of the relationships between oligonucleotide properties and hybridization signal intensities from NimbleGen microarray datasets

    Wei, H.   Kuan, P. F.   Tian, S.   Yang, C.   Nie, J.   Sengupta, S.   Ruotti, V.   Jonsdottir, G. A.   Keles, S.   Thomson, J. A.   Stewart, R.  

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  • Duality theorem for a generalized Fermat-Weber problem : Wilfred Kaplan and Wei H. Yang Mathematical Programming Vol. 76, 1997, 285–297

    Jones, Philip C.   Lowe, Timothy J.   Muller, Georg   Ning, Xu   Yinyu, Ye   Zydiak, James L.  

    This paper considers a specially structured uncapacitated facility location problem. We show that several problems, including certain tool selection problems, substitutable inventory problems, supplier sourcing problems, discrete lot sizing problems, and capacity expansion problems, can be formulated as instances of the problem. We also show that the problem with m facilities and n customers can be solved in O (mn), as a shortest path problem on a directed graph.
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  • Duality theorem for a generalized Fermat-Weber problem : Wilfred Kaplan and Wei H. Yang Mathematical Programming Vol. 76, 1997, 285–297

    Kaplan, Wilfred   Yang, Wei H.  

    The classical Fermat-Weber problem is to minimize the sum of the distances from a point in a plane to k given points in the plane. This problem was generalized by Witzgall to n-dimensional space and to allow for a general norm, not necessarily symmetric; he found a dual for this problem. The authors generalize this result further by proving a duality theorem which includes as special cases a great variety of choices of norms in the terms of the Fermat-Weber sum. The theorem is proved by applying a general duality theorem of Rockafellar. As applications, a dual is found for the multi-facility location problem and a nonlinear dual is obtained for a linear programming problem with a priori bounds for the variables. When the norms concerned are continuously differentiable, formulas are obtained for retrieving the solution for each primal problem from the solution of its dual.
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  • Duality theorem for a generalized Fermat-Weber problem : Wilfred Kaplan and Wei H. Yang Mathematical Programming Vol. 76, 1997, 285–297

    Nickel   Stefan  

    In this paper we look at two interesting extensions to the classical 2-facility Weber problem in Rd: at first problems are investigated where we do not allow the optimal locations to be in a specific region. Efficient algorithms for this global optimization problem are presented as well as new structural results. Secondly we consider 2-facility Weber problems with two decision makers where each decision maker can choose his own preferences for the location problem. We give an efficient algorithm for determining all pareto locations for this multicriteria problem as well as a polynomial description of the set of all pareto locations (in R2d). All the results presented in this paper are based on a discretization of the original continuous problem using geometrical and combinatorial arguments. The time complexity of all the presented algorithms is O (dM log M), where M is the number of existing facilities.
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  • The influence of chemical sympathectomy on pain responsivity and α2-adrenergic antinociception in neuropathic animals

    Wei, H.   Jyvä   sjä   rvi, E.   Niissalo, S.   Hukkanen, M.   Waris, E.   Konttinen, Y.T.   Pertovaara, A.  

    We studied the effect of chemical sympathectomy by 6-hydroxydopamine (6-OHDA) on pain behavior and α2-adrenergic antinociception in rats with a spinal nerve ligation-induced neuropathy. For assessment of α2-adrenergic antinociception, the rats were treated systemically with two α2-adrenoceptor agonists, one of which only poorly (MPV-2426) and the other very well (dexmedetomidine) penetrates the blood–brain barrier. Moreover, the effect of MPV-2426 on spontaneous activity of dorsal root nerve fibers proximal to the nerve injury was determined. Systemic treatment with 6-OHDA produced a marked decrease in immunocytochemical labeling of sympathetic nerve fibers in the skin but it produced no marked change in basal pain sensitivity to mechanical stimulation either in neuropathic or sham-operated animals. Systemic administration of MPV-2426 and dexmedetomidine produced a dose-dependent tactile antiallodynic effect in neuropathic animals. Intraplantar injection of MPV-2426 had an identical antiallodynic effect independent of whether it was injected into the neuropathic or contralateral hindpaw. In a test of mechanical nociception and hyperalgesia, dexmedetomidine markedly attenuated pain responses in all experimental groups, whereas MPV-2426 had a weak but significant pain attenuating effect only in neuropathic animals. In the tail flick test, both α2-adrenoceptor agonists had a significant antinociceptive effect. The pain attenuating effect of MPV-2426 was enhanced by pretreatment with 6-OHDA, except in a test of tactile allodynia. MPV-2426-induced modulation of spontaneous activity was not a general property of dorsal root fibers proximal to the injury. The results indicate that a chemical destruction of sympathetic postganglionic nerve fibers innervating the skin does not markedly influence cutaneous pain sensitivity nor is it critical for the α2-adrenoceptor agonist-induced attenuation of pain behavior in neuropathic or non-neuropathic animals. Chemical sympathectomy, independent of neuropathy, enhanced the pain attenuating effect by MPV-2426, probably due to a peripheral action, whereas in non-sympathectomized control and neuropathic animals peripheral mechanisms have only a minor, if any, role in the α2-adrenoceptor agonist-induced antinociception.
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