Pieck, Carlos Ariel
Crampon, Christelle
Charton, Frederic
Badens, Elisabeth
In this work, the supercritical fractionation of fatty acid ethyl esters derived from fish oil was carried out with carbon dioxide at 333 K and 14.5 MPa. The feed mixture is very complex, with over 80 different compounds detected by GC. Among them, the EicosaPentaenoic Acid (EPA) and DodecaHexaenoic Acid (DHA) ethyl esters are of major industrial interest since the consumption of these omega-3 acids has been linked to a reduced risk of cardiovascular diseases. The influence of the process parameters on the separation efficiency is an important point for an economic analysis of the overall purification process. First, the partition of the feed mixture was determined for different solvent-to-feed ratios varying between 21.8 and 143; the extract yield (i.e. the mass fraction of feed recovered as extract) has been calculated for each condition. Then, a new simplified equilibrium-stage model was developed and applied to the mixture. Supposing a constant distribution ratio, this model is able to correlate the influence of the solvent-to-feed ratio (through a calculated extract yield) on the concentration of the major 24 compounds present either in the feed or in the extract and raffinate, including components with low, medium and high distribution ratios. (C) 2016 Elsevier B.V. All rights reserved.
This article deals with the extraction of neutral lipids and antioxidants from enriched Spirulina platensis microalgae using supercritical carbon dioxide (CO2), and more particularly with the influence of experimental conditions on extraction yields and kinetics at laboratory and pilot scales. Preliminary studies were carried out at laboratory scale before the establishment of an experimental design: extraction curves were plotted for different autoclave fill rates, and under different conditions of pressure and temperature. Using a Response Surface Methodology, the significant influence of pressure on extraction efficiency was highlighted. Surface responses showed that, in the studied experimental field, mass loss increased when pressure, temperature, and CO2/microalgae mass ratio increased. Extract analyses showed that oil extracts contained chlorophylls a and b, as well as beta-carotene. Finally, larger-scale experiments were carried out with batches of 1 and 50 kg (scale-factors of 100 and 5000, respectively) and the results were consistent with those obtained at laboratory scale. (C) 2016 Elsevier B.V. All rights reserved.
Majerik, Viktor
Charbit, Gerard
Badens, Elisabeth
Horvath, Geza
Szokonya, Laszlo
Bosc, Nathalie
Teillaud, Eric
Oxeglitazar is a new orally administered poorly water soluble active substance used in the treatment of type II diabetes. Our research aimed to improve the bioavailability of this active substance using Supercritical Antisolvent (SAS) process. Oxeglitazar was coprecipitated with various solubilizing excipients: polyoxyethylene-polyoxypropylene block copolymers (Poloxamer 188 and 407), polyethylene glycol (PEG 8000) and polyvinilpyrrolidone (PVP K17) from six different solvents: ethanol (EtOH), tetrahydrofuran (THF), dichloromethane (DCM), chloroform (CHCl3), N-methyl-2-pyrrolidone (NMP), dimethylsulfoxide (DMSO) and two binary solvent Mixtures: EtOH/THF (50:50%, v/v) and EtOH/CHCl3 (50:50%, v/v). Formulations were compared in terms of particle morphology, crystallinity, polymorphic purity, residual solvent content, precipitation yield and dissolution kinetics. SAS formulations of oxeglitazar-PEG 8000, Poloxamer 188 and 407 contained acicular drug crystals that were partly embedded in polymeric spheres while experiments with PVP K17 resulted in quasi amorphous solid dispersions with high density and good flowability. In spite of the greater particle size, SAS formulations exhibited significantly greater dissolution rate compared to raw drug and physical mixtures. More than twice as much active substance was dissolved at 5 min from Poloxamer 407 and PVP K17 formulations than from unprocessed drug. In addition, SAS prepared Poloxamer 407 formulation from DCM Solution exhibited high polymorphic purity, good flow properties, acceptable precipitation yield and low residual solvent content. (C) 2006 Elsevier B.V. All rights reserved.
Zermane, Ahmed
Larkeche, Ouassila
Meniai, Abdeslam-Hassen
Crampon, Christelle
Badens, Elisabeth
The present study deals with the determination of optimal values of operating parameters such as temperature and pressure leading to the best yield of a supercritical CO2 extraction of essential oil from local rosemary plants, using the response surface methodology (RSM). The maximum of essential oil recovery percentage relative to the initial mass of leaf powder was 3.52 wt%, and was obtained at 313 K and 22 MPa. A second-order polynomial was used to express the oil recovery and the calculated mass of recovered oil using the RSM was very close to the experimental value, confirming the reliability of this technique. The chemical composition of the Algerian rosemary oil under the obtained optimal conditions (313 K and 22 MPa), determined by GC-MS analysis, revealed the presence of camphor (major compound) (52.12%), 1,8-cineole (9.65%), camphene (7.55%), alpha-pinene (6.05%), borneol (3.52%), aroma dendrene (2.11%), verbenone (1.97%), alpha-caryophyllene (1.71%), and others. (C) 2016 Academie des sciences. Published by Elsevier Masson SAS. All rights reserved.