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Now showing items 1 - 16 of 147

  • Drosophila as a Model Organism

    Yamaguchi, Masamitsu   Yoshida, Hideki  

    Drosophila melanogaster has been widely used in classical and modern genetics for more than 100 years. The history of the Drosophila model in the study of various aspects of life sciences will be summarized in this chapter. Furthermore, commonly used techniques and tools with Drosophila models will be briefly described, with a special emphasis on the advantages of Drosophila models in the study of various human diseases.
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  • Evolution and diversification of the plant gibberellin receptor GID1.

    Yoshida, Hideki   Tanimoto, Eiichi   Hirai, Takaaki   Miyanoiri, Yohei   Mitani, Rie   Kawamura, Mayuko   Takeda, Mitsuhiro   Takehara, Sayaka   Hirano, Ko   Kainosho, Masatsune   Akagi, Takashi   Matsuoka, Makoto   Ueguchi-Tanaka, Miyako  

    The plant gibberellin (GA) receptor GID1 shows sequence similarity to carboxylesterase (CXE). Here, we report the molecular evolution of GID1 from establishment to functionally diverse forms in eudicots. By introducing 18 mutagenized rice GID1s into a rice gid1 null mutant, we identified the amino acids crucial for GID1 activity in planta. We focused on two amino acids facing the C2/C3 positions of ent-gibberellane, not shared by lycophytes and euphyllophytes, and found that adjustment of these residues resulted in increased GID1 affinity toward GA4, new acceptance of GA1 and GA3 carrying C13-OH as bioactive ligands, and elimination of inactive GAs. These residues rendered the GA perception system more sophisticated. We conducted phylogenetic analysis of 169 GID1s from 66 plant species and found that, unlike other taxa, nearly all eudicots contain two types of GID1, named A- and B-type. Certain B-type GID1s showed a unique evolutionary characteristic of significantly higher nonsynonymous-to-synonymous divergence in the region determining GA4 affinity. Furthermore, these B-type GID1s were preferentially expressed in the roots of Arabidopsis, soybean, and lettuce and might be involved in root elongation without shoot elongation for adaptive growth under low-temperature stress. Based on these observations, we discuss the establishment and adaption of GID1s during plant evolution. Copyright =C2=A9 2018 the Author(s). Published by PNAS.
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  • Soft Tissues: Lipoblastoma with t(2;8)(q31;q12.1) COL3A1/PLAG1

    Yoshida, Hideki   Miyachi, Mitsuru   Hosoi, Hajime  

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  • DREF plays multiple roles during Drosophila development

    Nguyen Trong Tue   Yoshioka, Yasuhide   Mizoguchi, Megumi   Yoshida, Hideki   Zurita, Mario  

    DREF was originally identified as a transcription factor that coordinately regulates the expression of DNA replication- and proliferation-related genes in Drosophila. Subsequent studies demonstrated that DREF is involved in tumor suppressor pathways including p53 and Hippo signaling. DREF also regulates the expression of genes encoding components of the JNK and EGFR pathways during Drosophila development. DREF itself is under the control of the TOR pathway during cell and tissue growth responding to nutrition. Recent studies revealed that DREF plays a role in chromatin organization including insulator function, chromatin remodeling, and telomere maintenance. DREF is also involved in the regulation of genes related to mitochondrial biogenesis, linking it to cellular proliferation. Thus, DREF is now emerging as not only a transcription factor, but also a multi-functional protein. In this review, we summarize current advances in studies on the novel functions of Drosophila DREF.
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  • MEMORY SYSTEM, HOST DEVICE AND INFORMATION PROCESSING SYSTEM

    According to one embodiment, a memory system includes a nonvolatile memory and a controller which controls the nonvolatile memory. The controller notifies to an outside an extensive signal which indicates a predetermined state of the nonvolatile memory or the controller.
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  • PIPING MEMBER, AND CONNECTING OR SEALING STRUCTURE USING THIS MEMBER

    A piping member (1, 23) having a flange structure comprising a tubular part (3, 25) inserted and fitted to the end of any pipe and a disk-shaped flange (2, 24) extending in the circumferential direction from the tubular part. A through-hole (4) in the central part of the flange communicates with the interior of the tubular part, and seal members (5) are mounted in seal member grooves (6) formed in the outer circumferential surface of the tubular part. An annular spacer (26) is arranged on the surface of the flange on the tubular-part side of the flange, and through-holes (27) for insertion of a pushing rod for pressing the spacer are provided in the flange.
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  • MONITORING SYSTEM AND SUBORDINATE DEVICE FOR PHOTOVOLTAIC EQUIPMENT

    A monitoring system includes a subordinate device (4) and a parent device (5). The child device (4) superimposes on a direct-current current path current signals showing measurement data, in which are measured each of more than one solar cell panel included in a plurality of solar cell panels (P1-P15) constituting a solar cell string (10). The direct-current current path includes a plurality of power lines (L1-L14), a first trunk power line (21), and a second trunk power line (22). The parent device (5) is linked to the first trunk power line (21) or the second trunk power line (22), or both, and receives the measurement data from the subordinate device (4). By this means, for example, communication performance can be improved between the subordinate device and the parent device in a monitoring system for monitoring solar cell panel units.
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  • Identification of a novel BOC-PLAG1 fusion gene in a case of lipoblastoma

    Nitta, Yoshihiro   Miyachi, Mitsuru   Tomida, Akimasa   Sugimoto, Yohei   Nakagawa, Norio   Yoshida, Hideki   Ouchi, Kazutaka   Tsuchiya, Kunihiko   Iehara, Tomoko   Konishi, Eiichi   Umeda, Katsutsugu   Okamoto, Takeshi   Hosoi, Hajime  

    Lipoblastoma is a rare benign adipose tissue tumor that occurs mostly in infants and children. Histo-logical diagnosis of lipoblastoma is sometimes difficult because it closely resembles other lipomatous tumors. The detection of PLAG1 gene rearrangement is useful for the diagnosis of lipoblastoma. Four PLAG1 fusion partner genes are known in lipoblastoma: HAS2 at 8q24.1, COL1A2 at 7q22, COL3A1 at 2q32, and RAB2A at 8q12. Herein, we describe a novel fusion gene in a case of lipoblastoma of left back origin. We identified a potential PLAG1 fusion partner using 5' rapid amplification of cDNA ends, and sequence analysis revealed the novel fusion gene, BOC-PLAG1. The BOC-PLAG1 fusion transcript consists of the first exon of the BOC gene fused to exon 2 or exon 3 of the PLAG1 gene. PLAG1 expression was found to be 35.7 +/- 2.1 times higher in the tumor specimen than in human adipocytes by qRT-PCR. As a result of the translocation, the constitutively active promoter of BOC leads to PLAG1 overexpression. The identification of the BOC-PLAG1 fusion gene will lead to more accurate diagnosis of lipoblastoma. (C) 2019 Elsevier Inc. All rights reserved.
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  • The Histone Deacetylase Gene Rpd3 Is Required for Starvation Stress Resistance

    Nakajima, Ei   Shimaji, Kouhei   Umegawachi, Takanari   Tomida, Saki   Yoshida, Hideki   Yoshimoto, Nana   Izawa, Shingo   Kimura, Hiroshi  

    Epigenetic regulation in starvation is important but not fully understood yet. Here we identified the Rpd3 gene, a Drosophila homolog of histone deacetylase 1, as a critical epigenetic regulator for acquiring starvation stress resistance. Immunostaining analyses of Drosophila fat body revealed that the subcellular localization and levels of Rpd3 dynamically changed responding to starvation stress. In response to starvation stress, the level of Rpd3 rapidly increased, and it accumulated in the nucleolus in what appeared to be foci. These observations suggest that Rpd3 plays a role in regulation of rRNA synthesis in the nucleolus. The RT-qPCR and ChIP-qPCR analyses clarified that Rpd3 binds to the genomic region containing the rRNA promoters and activates rRNA synthesis in response to starvation stress. Polysome analyses revealed that the amount of polysomes was decreased in Rpd3 knockdown flies under starvation stress compared with the control flies. Since the autophagy-related proteins are known to be starvation stress tolerance proteins, we examined autophagy activity, and it was reduced in Rpd3 knockdown flies. Taken together, we conclude that Rpd3 accumulates in the nucleolus in the early stage of starvation, upregulates rRNA synthesis, maintains the polysome amount for translation, and finally increases stress tolerance proteins, such as autophagy-related proteins, to acquire starvation stress resistance.
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  • Identification of a novel BOC-PLAG1 fusion gene in a case of lipoblastoma

    Nitta, Yoshihiro   Miyachi, Mitsuru   Tomida, Akimasa   Sugimoto, Yohei   Nakagawa, Norio   Yoshida, Hideki   Ouchi, Kazutaka   Tsuchiya, Kunihiko   Iehara, Tomoko   Konishi, Eiichi   Umeda, Katsutsugu   Okamoto, Takeshi   Hosoi, Hajime  

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  • COOLING UNIT FOR HEAT EXCHANGER

    The cooling unit of the present invention for injecting air toward a heat exchanger installed in a front part of a vehicle is provided with a blower and an air injection frame for injecting air supplied from the blower toward the heat exchanger, the air injection frame being installed in front of the heat exchanger. The air injection frame is provided with a side duct through which the air supplied from the blower is channeled, and a plurality of injection ducts arranged in the vertical direction in front of the heat exchanger and communicated at one end with the side duct. An ejector nozzle for injecting air diverted from the side duct toward the heat exchanger is formed in each of the injection ducts. This cooling unit makes it possible to pass air through the entire area of the heat exchanger and enhance the efficiency of heat exchange.
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  • COOLING MODULE

    This cooling module (1) mounted in a vehicle (3) is provided with a duct (5) arranged in the vehicle (3), a radiator (7) provided in the duct (5), a condenser (11) disposed in front of the radiator (7) in the duct (5), an air amount adjustment unit (12) which adjusts the amount of air flowing to the radiator (7) depending on the travel state of the vehicle (3), and a fan unit (14) which creates a flow of forced air in the duct (5).
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  • EL DISPLAY DEVICE

    This EL display device is provided with: a light-emitting unit at which a plurality of pixels are arrayed, each pixel being configured from subpixels that emit light at least at the light emission colors of red, green, and blue; and a thin film transistor array device that controls the light emission of the light-emitting unit. Each subpixel (51R, 51G, 51B, 51b) of the pixels (50) is configured in a manner so as to dispose the light emitting layers for at last red, green, and blue in regions demarcated in a grid by means of a bank. Subpixels (51R, 51G, 51B, 51b) having the same color formed in adjacent pixels (50) are configured in a manner so that the light emission layer is disposed within a first joined bank (52) having an area resulting from joining the banks for two subpixels.
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  • ELECTRONIC DEVICE

    An electronic device, such as a flexible EL display apparatus, wherein the present technology is used has: a first barrier film (24) having laminated therein an inorganic film (22) formed of an inorganic material, and a polymer film (23) formed of a polymer material; and a second barrier film (29) having laminated therein an inorganic film (27) formed of an inorganic material and a polymer film (28) formed of a polymer material. The electronic device is provided with a configuration wherein a thin film transistor array apparatus (25) and a light emitting section (26) are sealed using the first barrier film (24) and the second barrier film (29). Each of the polymer films (23, 28) is configured of the material containing at least one kind of nano fine particles in the polymer material, said nano fine particles being selected from the group consisting of silica-based particles, Fe-based particles, montmorillonite particles, silica-coated particles, and zeolite particles.
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  • DIMMING METHOD AND DIMMING DEVICE FOR LED

    [Problem] To provide an LED dimming device having satisfactory responsiveness and in which luminance unevenness, flickering and the like caused from noise can be prevented by generating a pulse signal of a TTL input/output level synchronized with a phase-controlled power wave and providing the pulse signal to the LED. [Solution] An LED dimming method for dimming an LED using a phase-controlled power wave from an AC power supply, said method comprising subjecting the phase-controlled power wave to full-wave rectification, then smoothly slicing the full-wave rectified wave to generate a first pulse signal dropped to a TTL input/output level, generating a second pulse signal shaped to a rectangular shape by making the edges of the first pulse signal steep, and providing the second pulse signal to the LED to carry out dimming control synchronized with the phase-controlled power wave.
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  • Dyskeratosis Congenita Complicated by Hepatic Fibrosis With Hepatic Vein Thrombosis

    Yoshida, Hideki   Imamura, Toshihiko   Sakamoto, Kenichi   Asai, Daisuke   Nakatani, Takuya   Morimoto, Akira   Hosoi, Hajime  

    We describe the case of 2 male siblings with dyskeratosis congenita (DC). Extensive genetic analysis failed to identify a causative genetic abnormality. The elder brother developed hepatic fibrosis accompanied with hepatic vein thrombosis at the age of 9 years. Recent studies have found that patients with DC sometimes develop hepatic complications, including cirrhosis. However, little is known about hepatic complications in patients with DC who lack these mutations. Further genetic studies are required to understand the relationship between DC and hepatic complications. In addition, although danazol can sometimes be effective for treating bone marrow failure, hepatotoxicity can be a major complication. Therefore, when danazol is administered to patients with DC, careful monitoring for hepatic complications is important.
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