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Now showing items 1 - 16 of 615

  • A critical plane-energy model for multiaxial fatigue life prediction

    Wei, H.   Liu, Y.  

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  • p62/SQSTM1 synergizes with autophagy for tumor growth in vivo

    Wei, H.   Wang, C.   Croce, C. M.   Guan, J.-L.  

    Autophagy is crucial for cellular homeostasis and plays important roles in tumorigenesis. FIP200 (FAK family-interacting protein of 200 kDa) is an essential autophagy gene required for autophagy induction, functioning in the ULK1-ATG13-FIP200 complex. Our previous studies showed that conditional knockout of FIP200 significantly suppressed mammary tumorigenesis, which was accompanied by accumulation of p62 in tumor cells. However, it is not clear whether FIP200 is also required for maintaining tumor growth and how the increased p62 level affects the growth in autophagy-deficient FIP200-null tumors in vivo. Here, we describe a new system to delete FIP200 in transformed mouse embryonic fibroblasts as well as mammary tumor cells following their transplantation and show that ablation of FIP200 significantly reduced growth of established tumors in vivo. Using similar strategies, we further showed that either p62 knockdown or p62 deficiency in established FIP200-null tumors dramatically impaired tumor growth. The stimulation of tumor growth by p62 accumulation in FIP200-null tumors is associated with the up-regulated activation of the NF-kappa B pathway by p62. Last, we showed that overexpression of the autophagy master regulator TFEBS142A increased the growth of established tumors, which correlated with the increased autophagy of the tumor cells. Together, our studies demonstrate that p62 and autophagy synergize to promote tumor growth, suggesting that inhibition of both pathways could be more effective than targeting either alone for cancer therapy.
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  • PRMT5 dimethylates R30 of the p65 subunit to activate NF-?B

    Wei, H.   Wang, B.   Miyagi, M.   She, Y.   Gopalan, B.   Huang, D.-B.   Ghosh, G.   Stark, G. R.   Lu, T.  

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  • Physical Symptoms and Associated Factors in Chinese Renal Transplant Recipients

    Wei, H.   Guan, Z.   Zhao, J.   Zhang, W.   Shi, H.   Wang, W.   Wang, J.   Xiao, X.   Niu, Y.   Shi, B.  

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  • Highly tunable propagating surface plasmons on supported silver nanowires

    Wei, H.   Zhang, S.   Tian, X.   Xu, H.  

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  • Suppression of autophagy by FIP200 deletion inhibits mammary tumorigenesis

    Wei, H.   Wei, S.   Gan, B.   Peng, X.   Zou, W.   Guan, J.-L.  

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  • Largest ever screening study for BRCA1/2 Mutations in breast cancer patients in China

    Wei, H.   Huang, Y.   Tian, F.   Ling, R.   Ou, J.   Liu, J.   Sheng, Y.   Liao, N.   Chen, Z.   Jiang, J.  

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  • Dimerization of a viral SET protein endows its function

    Wei, H.   Zhou, M.-M.  

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  • Fratricide Is Essential for Efficient Gene Transfer between Pneumococci in Biofilms

    Wei, H.   Havarstein, L. S.  

    Streptococcus pneumoniae and a number of commensal streptococcal species are competent for natural genetic transformation. The natural habitat of these bacteria is multispecies biofilms in the human oral cavity and nasopharynx. Studies investigating lateral transfer of virulence and antibiotic resistance determinants among streptococci have shown that interspecies as well as intraspecies gene exchange takes place in these environments. We have previously shown that the action of a competence-specific murein hydrolase termed CbpD strongly increases the rate of gene transfer between pneumococci grown in liquid cultures. CbpD is the key component of a bacteriolytic mechanism termed the fratricide mechanism. It is secreted by competent pneumococci and mediates the release of donor DNA from sensitive streptococci present in the same environment. However, in nature, gene exchange between streptococci takes place in biofilms and not in liquid cultures. In the present study, we therefore investigated whether CbpD affects the rate of gene transfer in laboratory-grown biofilms. Our results show that the fratricide mechanism has a strong positive impact on intrabiofilm gene exchange, indicating that it is important for active acquisition of homologous donor DNA under natural conditions. Furthermore, we found that competent biofilm cells of S. pneumoniae acquire a Nov(r) marker much more efficiently from neighboring cells than from the growth medium. Efficient lysis of target cells requires that CbpD act in conjunction with the murein hydrolase LytC. In contrast, the major autolysin LytA does not seem to be important for fratricide-mediated gene exchange in a biofilm environment.
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  • Cancer Immunotherapy Using In vitro Genetically Modified Targeted Dendritic Cells

    Wei, H.   Wang, H.   Lu, B.   Li, B.   Hou, S.   Qian, W.   Fan, K.   Dai, J.   Zhao, J.   Guo, Y.  

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  • Protective effects of epigallocatechin-3-gallate against lead-induced oxidative damage

    Wei, H.   Meng, Z.  

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  • Formation of porous hydrophobic stainless steel surfaces by maskless electrochemical machining

    Wei, H.   Wang, G.   Liu, G.   Guo, Z.  

    Surface topography is an important feature, which directly affects the friction, corrosion and flow characteristics of a workpiece. In this study, a new approach was explored to produce a microporous structure by maskless electrochemical machining (ECM). A rough surface with a microporous structure was created on a stainless steel substrate, and the principle of the micropore formation was investigated. It was found that the microporosity could form, grow and interconnect when the potential of the anodic surface was higher than the pitting potential of the material. The electric field is an important factor, and the surface topography can be altered by changing the electrical parameters. The porous microstructure was observed by laser scanning confocal microscopy. The rough and porous surface, modified with 1-dodecanethiol, exhibits hydrophobic characteristics, with a static contact angle of 137° for water droplets. This is a significant improvement in the wetting properties for stainless steels.
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  • Transcriptional Coordination of the Metabolic Network in Arabidopsis

    Wei, H.   Persson, S.   Mehta, T.   Srinivasasainagendra, V.   Chen, L.   Page, G. P.   Somerville, C.   Loraine, A.  

    Patterns of coexpression can reveal networks of functionally related genes and provide deeper understanding of processes requiring multiple gene products. We performed an analysis of coexpression networks for 1,330 genes from the AraCyc database of metabolic pathways in Arabidopsis (Arabidopsis thaliana). We found that genes associated with the same metabolic pathway are, on average, more highly coexpressed than genes from different pathways. Positively coexpressed genes within the same pathway tend to cluster close together in the pathway structure, while negatively correlated genes typically occupy more distant positions. The distribution of coexpression links per gene is highly skewed, with a small but significant number of genes having numerous coexpression partners but most having fewer than 10. Genes with multiple connections (hubs) tend to be single-copy genes, while genes with multiple paralogs are coexpressed with fewer genes, on average, than single-copy genes, suggesting that the network expands through gene duplication, followed by weakening of coexpression links involving duplicate nodes. Using a network-analysis algorithm based on coexpression with multiple pathway members (pathway-level coexpression), we identified and prioritized novel candidate pathway members, regulators, and cross pathway transcriptional control points for over 140 metabolic pathways. To facilitate exploration and analysis of the results, we provide a Web site (http://www.transvar.org/at_coexpress/analysis/web) listing analyzed pathways with links to regression and pathway-level coexpression results. These methods and results will aid in the prioritization of candidates for genetic analysis of metabolism in plants and contribute to the improvement of functional annotation of the Arabidopsis genome.
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  • Interference-free broadband single- and multicarrier DS-CDMA

    Wei, H.   Yang, L.L.   Hanzo, L.  

    The choice of the direct sequence spreading code in DS-CDMA predetermines the properties of the system. This contribution demonstrates that the family of codes exhibiting an interference-free window (IFW) outperforms classic spreading codes, provided that the interfering multi-user and multipath components arrive within this IFW, which may be ensured with the aid of quasi-synchronous adaptive timing advance control. It is demonstrated that the IFW duration may be extended with the advent of multicarrier DS-CDMA proportionately to the number of subcarriers. Hence, the resultant MC DS-CDMA system is capable of exhibiting near-single-user performance without employing a multi-user detector. A limitation of the system is that the number of spreading codes exhibiting a certain IFW is limited, although this problem may be mitigated with the aid of novel code design principles
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  • Effects of Traditional Chinese Medicine Wuzhi Capsule on Pharmacokinetics of Tacrolimus in Rats

    Wei, H.   Tao, X.   Di, P.   Yang, Y.   Li, J.   Qian, X.   Feng, J.   Chen, W.  

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  • Quantifying stacking faults and vacancies in thin convectively assembled colloidal crystals

    Wei, H.   Meng, L.   Jun, Y.   Norris, D. J.  

    The authors determine the concentration and distribution of stacking faults and vacancies in colloidal crystals between 3 and 11 layers thick grown via convective assembly. The average stacking parameter rises from 0.1 for samples 3 layers thick to 0.8 for >= 6 layers. For samples >= 6 layers, the authors also find that the stacking faults are less likely to be near the substrate. The average vacancy concentration is 2.1 x 10(-3) per unit cell. Surprisingly, the layer-averaged vacancy concentration is lowest in the layer nearest the substrate and highest in the next nearest layer. (c) 2006 American Institute of Physics.
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