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Now showing items 1 - 16 of 31

  • Glycosaminoglycans from bovine eye vitreous humour and interaction with collagen type II

    Peng, Yanfei   Yu, Yanlei   Lin, Lei   Liu, Xinyue   Zhang, Xing   Wang, Peipei   Hoffman, Pauline   Kim, So Young   Zhang, Fuming   Linhardt, Robert J.  

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  • Prohibitin-2 negatively regulates AKT2 expression to promote prostate cancer cell migration

    Shen, Yongmei   Gao, Yu   Yuan, Hui   Cao, Jiasong   Jia, Bona   Li, Mingming   Peng, Yanfei   Du, Xiaoling   Zhang, Ju   Shi, Jiandang  

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    A cementitious board system which is reinforced on its opposed surfaces by an improved glass fiber mesh scrim with thicker yarn and larger mesh openings to provide a cementitious board with improved handling properties while retaining tensile strength and long term durability. The fabric is constructed as a mesh of high modulus strands of bundled glass fibers encapsulated by alkali and water resistant material, e.g. a thermoplastic material. The composite fabric also has suitable physical characteristics for embedment within the cement matrix of the panels or boards closely adjacent the opposed faces thereof. The fabric provides a board system with long- lasting, high strength tensile reinforcement and improved handling properties regardless of their spatial orientation during handling. Also included are methods for making the reinforced board.
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  • Structure and antitumor activity of extracellular polysaccharides from mycelium

    Peng, Yanfei   Zhang, Lina   Zeng, Fanbo   Xu, Yanxia  

    Two heteropolysaccharides coded as EPF1 and EPF2 were obtained from the crude extracellular polysaccharide (CEP) of Ganoderma tsugae mycelium by elution with DEAE-Sepharose CL-6B column. Their chemical structures and molecular mass were characterized by infrared, gas chromatography, 13C NMR and size exclusion chromatography combined with laser light scattering (SEC-LLS). The results indicated that they were mainly composed with mannose, fucose, xylose, galactose and glucosamine. EPF2 was confirmed to be mainly a β-d-galacto-α-d-mannan. The weight-average molecular mass of the samples EPF1, EPF2 and CEP were 92.0×104, 8.35×104 and 23.8×104, respectively. The EPF1 existed as a compact coil chain in 0.2 M NaCl aqueous solution at 25 °C, while EPF2 as a flexible chain. The antitumor activities against Sarcoma 180 were tested both in vitro and in vivo. All the samples exhibited high inhibition ratio against Sarcoma 180 in mice, and the CEP has higher inhibition effect both in vivo and in vitro than EPF1 and EPF2. In view of these results, the presence of bound protein and mannose, and relatively expanded chain would be helpful to the enhancement of antitumor activities. This study suggested that the extracellular polysaccharides had a potential application as natural antitumor drugs.
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  • Chain Conformation of an Alkali‐Soluble Polysaccharide from Mycelium of Ganoderma tsugae

    Peng, Yanfei   Zhang, Lina  

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    Disclosed are hydrophobic finish compositions and cementitious articles made with the hydrophobic finish compositions. In some embodiments, the article is a waterproof gypsum panel surface reinforced with inorganic mineral fibers that face a flexible and hydrophobic cementitious finish possessing beneficial waterproofing properties. These waterproof gypsum panels have many uses, such as, tile backer board in wet or dry areas of buildings, exterior weather barrier panel for use as exterior sheathing, interior wall and ceiling, and roof cover board having water durability and low surface absorption. The flexible and hydrophobic cementitious finish can include fly ash, film-forming polymer, preferably silane compound (e.g., alkyl alkoxysilane), an extended flow time retention agent including either one or more carboxylic acids, salts of carboxylic acids, or mixtures thereof, and other optional additives. Preferably a pre-coated non-woven glass fiber mat is employed to provide the inorganic mineral fibers for the surface reinforcement.
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    Disclosed are a battery charging/discharging method and circuit, falling within the technical field of electronics. The battery charging/discharging circuit comprises a power supply, a load and at least one battery pack, wherein a first electrode of the power supply and a first electrode of the load are connected to a first node, and a second electrode of the power supply and a second electrode of the load are connected to a second node. The charging/discharging circuit further comprises loop circuits corresponding to battery packs on a one-to-one basis, wherein a first electrode of a battery pack is connected to the first node, and a loop circuit is located between a second electrode of the battery pack and the second node. A loop assembly comprises: a first switch, a second switch and a diode, wherein a first end of the first switch and a first end of the second switch are both connected to a second electrode of a battery pack; a second end of the second switch is connected to a first electrode of the diode; and a second end of the first switch and a second electrode of the diode are connected to a third node. The technical solution can effectively solve the problems that a lithium-iron battery cannot be subjected to floating charging for a long time and charging/discharging is uncontrollable due to a transient large current during a battery switching-on period.
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    Disclosed are cementitious articles with hydrophobic finish. In some embodiments, the article is a waterproof gypsum panel that is surface reinforced with inorganic mineral fibers that face a flexible and hydrophobic cementitious finish possessing beneficial waterproofing properties. The waterproof gypsum panels of the invention are useful in many applications, such as, for example, tilebacker board in wet or dry areas of buildings, exterior weather barrier panel for use as exterior sheathing, and roof cover board having superior water durability and extremely low surface absorption. The flexible and hydrophobic cementitious finish of the invention can include Class C fly ash, film-forming polymer, silane compound (e.g., alkyl alkoxysilane), and other optional additives.
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  • Detection of sialylated N-Linked glycans by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

    Peng, Yanfei   Xu, Xiaojuan  

    Native and methyl-esterified sialylated glycans were analyzed with 2, 4, 6-trihydroxyacetophenone (THAP) and 2, 5-dihydroxybenzoic acid (DHB) as matrix by a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF MS). High quality negative-ion spectra of commercial sialylated glycan were obtained with THAP as matrix. Detection limit of the glycan was less than 0.1 pmol. After methyl esterification of sialic acid (SA) residue, sialylated glycans were detected sensitively in the positive-ion mode using DHB as matrix. Neutral and sialylated glycans from the mixture of asialofetuin and fetuin were methylesterified and simultaneously recognized in one manipulation. Methyl esterification of SA residue offers a convenient and sensitive way to identify the structure of N-linked glycans for glycan profiling.
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    An internal resistance detection device and detection method for a storage battery, and a storage battery state monitoring system. The detection device (100) comprises: a discharging unit (101), connected to a storage battery (105), and set to discharge the storage battery (105), wherein a discharging current is within a pre-set range; a discharging control unit (102), connected to the discharging unit (101), and set to repeatedly control the conduction of the discharging unit (101) and close same after a pre-set time, and to perform pulse discharging on the storage battery (105) a plurality of times; a data collection unit (103), respectively connected to the storage battery (105), and set to acquire, in real time, a current value and a voltage value during the process of performing pulse discharging on the storage battery (105); and a data processing unit (104), connected to the data collection unit (103), and set to calculate an internal resistance of the storage battery (105) according to the voltage value and the current value, and the number of times that pulse discharging is performed. On the premise of ensuring a lower degree of damage caused by discharging to the storage battery (105), the technical solution enhances the authenticity and accuracy of the internal resistance measurement of the storage battery (105).
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  • Prostaglandin E2 induces stromal cell-derived factor-1 expression in prostate stromal cells by activating protein kinase A and transcription factor Sp1

    Peng, Yanfei   Shi, Jiandang   Du, Xiaoling   Wang, Liang   Klocker, Helmut   Mo, Linjian   Mo, Zengnan   Zhang, Ju  

    Recent reports indicate prostaglandin E2 (PGE2) can modulate tumor environment and promote angiogenesis through induction of stromal cell-derived factor 1 (SDF-1) production. We investigated the mechanism of PGE2-induced SDF-1 regulation in human prostate stromal cell and analyzed the effects in a stromal-epithelial interaction model. PGE2 stimulation increased SDF-1 expression in the prostate stromal cell lines WPMY-1 and NAF. We revealed signaling through the PGE2 receptor EP3 and activation of protein kinase A (PKA) are required. The EP3 agonist sulprostone and the cAMP analog forskolin mimicked and the EP3 siRNA, antagonist L798106 and the PKA inhibitor H89 abrogated the effect of PGE2 on SDF-1 expression. SDF-1 promoter truncation experiments demonstrated a 254 bp (from nt -219 to nt +34) SDF-1 proximal promoter fragment containing 5 putative transcription factor Sp1 binding motifs is sufficient for PGE2 induction. CHIP assays confirmed binding and PGE2 induced recruitment of Sp1 to the SDF-1 promoter. Sp1 motif mutation identified Sp1 motifs -140/-133 and -9/+1 as the crucial elements responsible for PGE2 induction. Moreover, SDF-1 was up- or down-regulated by Sp1 over-expression or knock-down. We also demonstrate stimulation of migration of prostate cancer cell lines PC3 and DU145 with conditioned media collected from WPMY-1 or NAF cells stimulated with PGE2 and blockade of enhanced migration by a SDF-1 neutralizing antibody. In conclusion, we provide evidence for a paracrine prostate stromal-epithelial interaction induced by upregulation of expression of SDF-1 by PGE2. Our research provides new insights into the mechanism promoting metastasis of prostate carcinoma via stromal-epithelial interaction. (c) 2012 Elsevier Ltd. All rights reserved.
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  • Characterization of a polysaccharide–protein complex from Ganoderma tsugae mycelium by size-exclusion chromatography combined with laser light scattering

    Peng, Yanfei   Zhang, Lina  

    A water-soluble polysaccharide–protein complex (GM3) extracted from the mycelium of Ganoderma tsugae was characterized using size-exclusion chromatography combined with laser light scattering (SEC-LLS). Two peaks coded as fractions I and II appeared in the SEC pattern of GM3 in 0.5 M NaCl aqueous solution, corresponding to the weight-average molecular mass (Mw) of 355×104 and 6.3×104, respectively. The relationship between the radius of gyration (<s2>z1/2) and Mw showed that molecules of fraction I exhibited more compact coil conformation than that of fraction II in 0.5 M NaCl aqueous solution at 25 °C. To clarify the component of polysaccharide and protein in each fraction, the sample GM3 was treated with 0.2 M NaOH aqueous solution to degrade polysaccharide and trypsin to hydrolyze protein. The obtained products were analyzed by SEC combined with detectors such as UV, differential refractive index (DRI) and LLS. The results indicated that both the fractions I and II were protein-bound polysaccharide, but had different protein content and degree of branching, resulting in the difference of the chain conformation.
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  • GPR30 Promotes Prostate Stromal Cell Activation via Suppression of ER alpha Expression and Its Downstream Signaling Pathway

    Jia, Bona   Gao, Yu   Li, Mingming   Peng, Yanfei   Du, Xiaoling   Klocker, Helmut   Sampson, Natalie   Shen, Yongmei   Liu, Mengyang  

    Cancer-associated fibroblasts (CAFs) play a vital role in malignant transformation and progression of prostate cancer (PCa), and accumulating evidence suggests an enhancing effect of estrogens on PCa. The present study aimed to investigate the possible origin of prostate CAFs and the effects of estrogen receptors, G protein-coupled receptor 30 (GPR30) and estrogen receptor (ER)-alpha, on stromal cell activation. High expression of fibroblast activation protein (FAP), CD44, and nonmuscle myosin heavy chain B (SMemb) accompanied by low expression of smooth muscle differentiation markers was found in the stromal cells of PCa tissues and in cultured human prostate CAFs. Additionally, SMemb expression, which is coupled to cell phenotype switching and proliferation, was coexpressed with FAP, a marker of activated stromal cells, and with the stem cell marker CD44 in the stromal cells of PCa tissue. Prostate CAFs showed high GPR30 and low ER alpha expression. Moreover, GPR30 was coexpressed with FAP, CD44, and SMemb. Furthermore, the study demonstrated that the overexpression of GPR30 or the knockdown of ER alpha in prostate stromal cells induced the up-regulation of FAP, CD44, Smemb, and the down-regulation of smooth muscle markers. The conditioned medium from these cells promoted the proliferation and migration of LNCaP and PC3 PCa cells. GPR30 knockdown or ER alpha overexpression showed opposite effects. Finally, we present a novel mechanism whereby GPR30 limits ER alpha expression via inhibition of the cAMP/protein kinase A signaling pathway. These results suggest that stem-like cells within the stroma are a possible source of prostate CAFs and that the negative regulation of ER alpha expression by GPR30 is centrally involved in prostate stromal cell activation.
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  • Estrogen receptor α-NOTCH1 axis enhances basal stem-like cells and epithelial-mesenchymal transition phenotypes in prostate cancer

    Shen, Yongmei   Cao, Jiasong   Liang, Zhixian   Lin, Qimei   Wang, Jianxi   Yang, Xu   Zhang, Ran   Zong, Jiaojiao   Du, Xiaoling   Peng, Yanfei   Zhang, Ju   Shi, Jiandang  

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  • Estradiol promotes epithelial-to-mesenchymal transition in human benign prostatic epithelial cells

    Shi, Xiaoyu   Peng, Yanfei   Du, Xiaoling   Liu, Haitao   Klocker, Helmut   Lin, Qimei   Shi, Jiandang   Zhang, Ju  

    BackgroundEpithelial-to-mesenchymal transition (EMT) is involved in pathogenesis of human benign prostatic hyperplasia (BPH). Estrogenic signaling pathways may stimulate the induction of EMT. However, the details of estradiol (E2) and estrogen receptors (ERs) effects on EMT, as well as E2-induced modulation of benign prostatic epithelial cell phenotype in vitro have not been completely clarified. MethodsThe effects of E2 on EMT markers and cytokeratins (CKs) expression were evaluated in benign epithelial cell lines BPH-1 and RWPE-1, which were cultured both in two-dimensional (2D) culture and three-dimensional (3D) culture model using hanging drop technique or 3D Matrigel model. ER antagonist, ICI182,780, was used to confirm the regulatory effects of E2 on EMT and phenotypic modulation. In 3D culture, immunohistochemical stainings were performed to detect the specific phenotype of cells that underwent EMT in acinar-like spheroids formed by RWPE-1. To illustrate the exact function of ERs in E2-induced EMT and phenotypic modulation, specific short interfering RNAs (siRNAs), and agonists were used to knockdown or activate individual ERs, respectively. ResultsE2-induced EMT was observed both in 2D and 3D culture, with related regulation of EMT markers expression at both mRNA and protein level. In addition, E2 down-regulated luminal cell type markers CK18 and CK8 and up-regulated basal cell type markers CK5 and CK14. E2 also increased intermediate type markers CK15 and CK17, while it attenuated CK19 in 3D culture. ICI182,780 blocked E2-induced EMT and cell phenotypic switching. In 3D Matrigel culture, Vimentin was co-expressed with ER and CK17, as well as with SMemb, which is related to cell status switching and proliferation. Knockdown of ER but not GPR30 inhibited EMT, while ER knockdown facilitated EMT process. Knockdown of ER blocked E2-induced EMT both in RWPE-1 and BPH-1. MRNA expression of EMT markers was stimulated by ER-specific agonist PPT and inhibited by ER-specific agonist DPN. ConclusionsEstrogenic effect mediated by ER can promote EMT. E2 is also an inductive factor of cell phenotypic switching. Cell type modulation is associated with E2-induced EMT in benign prostatic epithelial cells. Taken together the results support a contribution of estrogens to the pathogenesis of BPH in elderly men.
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  • Dietary Natural N-Acetyl-D-Glucosamine Prevents Bone Loss in Ovariectomized Rat Model of Postmenopausal Osteoporosis

    Jiang, Zhiwen   Li, Zhe   Zhang, Wei   Yang, Yan   Han, Baoqin   Liu, Wanshun   Peng, Yanfei  

    Postmenopausal osteoporosis has seriously affected the life quality of elderly women. A natural polymer, chitin, obtained from shells of crab and shrimp, has been widely used in the biomedical field owing to its nontoxicity, biocompatibility, and biodegradability. In this study, natural N-acetyl-D-glucosamine (NAG) was prepared from liquefied chitin. The protective activities of NAG in postmenopausal osteoporosis were evaluated on Sprague Dawley rats and osteoblast-based models. Results showed that oral administration of NAG boosted trabecular bone volume and trabecular numbers. Additionally, the calcium content in the femur and tibia increased, and femoral biomechanical properties improved. Furthermore, NAG supplementation significantly lowered alkaline phosphatase levels and increased calcium content in the serum of ovariectomized rats. In vitro studies showed that NAG markedly promoted cell proliferation and stimulated osteoblast differentiation of mouse calvaria origin MC3T3-E1 cells with increased alkaline phosphatase activity in a concentration-dependent manner. Moreover, NAG effectively protected osteoblasts from oxidative damage induced by hydrogen peroxide. In conclusion, our data provide an additional foundation for dietary supplementation of NAG, which could protect and reverse osteopenia in postmenopausal women.
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