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Now showing items 1 - 9 of 9

  • CXCR4 overexpression is correlated with poor prognosis in colorectal cancer

    Chao Xu   Linfeng Zheng   Dechuan Li   Guoping Chen   Jianzhong Gu   Jun Chen   Qinghua Yao  

    Abstract Aims Colorectal cancer threatens human health due to its high mortality resulting from metastatic progression. The expression of C-X-C chemokine receptor type 4 (CXCR4) is absent or low in most healthy tissues but high in various types of tumours. In this study, we aim to determine the prognostic significance of CXCR4 in colorectal cancer. Main methods We retrospectively examined a total of 72 tissue samples, that qRT-PCR and immunohistochemistry were performed to detect the expression of CXCR4 as well as univariate and multivariate analyses were performed to explore the overall survival. Key findings Our data demonstrated that CXCR4 expression was associated with lymph node metastasis ( P  = 0.049), histological differentiation ( P  = 0.01), distant metastasis ( P  = 0.02) and DNA mismatch repair (MMR) index ( P  = 0.0002). However, CXCR4 expression was not associated with age, sex, tumour diameter or depth of invasion. Furthermore, both univariate and multivariate analyses confirmed that CXCR4 was an independent factor in predicting unfavourable overall survival (hazard ratio, 0.188; 95% confidence interval, 0.038–0.757). Significance In conclusion, our findings suggest that CXCR4 might contribute to clinical tumour progression and may be a valuable prognostic biomarker in colorectal cancer treatment. Graphical abstract Unlabelled Image
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  • Magnetic resonance imaging of glioma with novel APTS-coated superparamagnetic iron oxide nanoparticles

    Kangan Li   Mingwu Shen   Linfeng Zheng   Jinglong Zhao…  

    We report in vitro and in vivo magnetic resonance (MR) imaging of C6 glioma cells with a novel acetylated 3-aminopropyltrimethoxysilane (APTS)-coated iron oxide nanoparticles (Fe3O4 NPs). In the present study, APTS-coated Fe3O4 NPs were formed via a one-step hydrothermal approach and then chemically modified with acetic anhydride to generate surface charge-neutralized NPs. Prussian blue staining and transmission electron microscopy (TEM) data showed that acetylated APTS-coated Fe3O4 NPs can be taken up by cells. Combined morphological observation, cell viability, and flow cytometric analysis of the cell cycle indicated that the acetylated APTS-coated Fe3O4 NPs did not significantly affect cell morphology, viability, or cell cycle, indicating their good biocompatibility. Finally, the acetylated APTS-coated Fe3O4 nanoparticles were used in magnetic resonance imaging of C6 glioma. Our results showed that the developed acetylated APTS-coated Fe3O4 NPs can be used as an effective labeling agent to detect C6 glioma cells in vitro and in vivo for MR imaging. The results from the present study indicate that the developed acetylated APTS-coated Fe3O4 NPs have a potential application in MR imaging.
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  • Differentiation between adrenal adenomas and nonadenomas using dynamic contrast-enhanced computed tomography

    Xifu Wang   Kangan Li   Haoran Sun   Jinglong Zhao   Linfeng Zheng   Zhuoli Zhang   Renju Bai   Guixiang Zhang  

    This study was performed to evaluate the findings including the time density curve (TD curve), the relative percentage of enhancement washout (Washr) and the absolute percentage of enhancement washout (Washa) at dynamic contrast-enhanced computed tomography (DCE-CT) in 70 patients with 79 adrenal masses (including 44 adenomas and 35 nonadenomas) confirmed histopathologically and/or clinically. The results demonstrated that the TD curves of adrenal masses were classified into 5 types, and the type distribution of the TD curves was significantly different between adenomas and nonadenomas. Types A and C were characteristic of adenomas, whereas types B, D and E were features of nonadenomas. The sensitivity, specificity and accuracy for the diagnosis of adenoma based on the TD curves were 93%, 80% and 87%, respectively. Furthermore, when myelolipomas were excluded, the specificity and accuracy for adenoma were 90% and 92%, respectively. The Washr and the Washa values for the adenomas were higher than those for the nonadenomas. The diagnostic efficiency for adenoma was highest at 7-min delay time at DCE-CT; Washr was more efficient than Washa. Washr ≥34% and Washa ≥43% were both suggestive of adenomas and, on the contrary, suspicious of nonadenomas. The sensitivity, specificity and accuracy for the diagnosis of adenoma were 84%, 77% and 81%, respectively. When myelolipomas were precluded, the diagnostic specificity and accuracy were 87% and 85%, respectively. Therefore, DCE-CT aids in characterization of adrenal tumors, especially for lipid-poor adenomas which can be correctly categorized on the basis of TD curve combined with the percentage of enhancement washout.
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  • Mefunidone ameliorates renal inflammation and tubulointerstitial fibrosis via suppression of IKKβ phosphorylation

    Jin Zhang   Linfeng Zheng   Xiangning Yuan   Chunyan Liu   Qiongjing Yuan   Feifei Xie   Sisi Qiu   Zhangzhe Peng   Yiting Tang   Jie Meng   Jiao Qin   Gaoyun Hu   Lijian Tao  

    Abstract Mefunidone is a new pyridone agent that attenuates renal tubulointerstitial fibrosis. However, the signaling pathways involved in the effect of mefunidone on renal tubulointerstitial fibrosis have not been well explained. Inflammatory response initiates and promotes renal tubulointerstitial fibrosis, and the inhibitor of nuclear factor kappa-B kinase beta (IKKβ) is a master regulator of inflammation. This study is determined to clarify the influence of mefunidone on renal inflammation and the phosphorylation of IKKβ. Experimental renal tubulointerstitial fibrosis was induced by unilateral ureteral obstruction (UUO) for 3, 7 and 14 days in sprague dawley rat. Treatment with mefunidone was conducted simultaneously. Obstructed kidneys were harvested for the assessment. Our results showed that treatment with mefunidone ameliorated renal inflammatory injury, renal tubular lesions and interstitial fibrosis. Further studies indicated that treatment with mefunidone mitigated the expressions of tumor necrosis factorα (TNFα) and interleukin-1β (IL-1β) in the kidney. The phosphorylation of IKKβ and inhibitor of kappa-B (IκB) and the expression of NOD-like receptor family, pyrin domain containing 3 (NALP3) were also reduced in vivo after treatment with mefunidone. In vitro, peritoneal macrophages were incubated with necrotic cells or lipopolysaccharide in the presence or absence of mefunidone. Mefunidone markedly decreased necrotic cell or LPS induced IL-1β production and LPS induced TNFα production in primary peritoneal macrophages. Furthermore, mefunidone significantly inhibited the phosphorylation of IKKβ/IκB and nuclear transition of NF-κB p65 in peritoneal macrophages stimulated by necrotic cell or lipopolysaccharide. In conclusion, mefunidone serves as a novel anti-inflammatory agent that attenuates UUO-induced renal interstitial inflammation and fibrosis, possibly through suppressing IKKβ phosphorylation.
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  • Targeted cancer theranostics using alpha-tocopheryl succinate-conjugated multifunctional dendrimer-entrapped gold nanoparticles

    Jingyi Zhu   Linfeng Zheng   Shihui Wen   Yueqin Tang   Mingwu Shen   Guixiang Zhang   Xiangyang Shi  

    Abstract Development of multifunctional theranostic nanoplatforms for targeted cancer imaging and therapy still remains a great challenge. Herein, we report the use of multifunctional dendrimer-entrapped gold nanoparticles (Au DENPs) covalently linked with α-tocopheryl succinate (α-TOS) as a platform for targeted cancer computed tomography (CT) imaging and therapy. In this study, amine-terminated poly(amidoamine) dendrimers of generation 5 (G5.NH 2 ) conjugated with fluorescein isothiocyanate (FI), polyethylene glycol (PEG)-modified α-TOS, and PEGylated folic acid (FA) were used as templates to synthesize Au DENPs, followed by acetylation of the remaining dendrimer terminal amines. The formed multifunctional Au DENPs were characterized via different techniques. We show that the Au DENPs conjugated with approximately 9.8 α-TOS molecules per dendrimer and with an Au core size of 3.3 nm are water-dispersible, and stable under different pH and temperature conditions and in different aqueous media. The FA modification onto the Au DENPs enables efficient targeting of the particles to cancer cells overexpressing FA receptors (FAR), and effective targeted CT imaging of the cancer cells in vitro and the xenografted tumor model in vivo . Likewise, the covalent conjugation of α-TOS does not compromise its therapeutic activity, instead significantly improves its water solubility. Importantly, thanks to the role of FA-directed targeting, the formed multifunctional Au DENPs are able to exert the specific therapeutic efficacy of α-TOS to the FAR-overexpressing cancer cells in vitro and the xenografted tumor model in vivo . The developed multifunctional Au DENPs may hold a great promise to be used as a unique theranostic nanoplatform for targeted CT imaging and therapy of different types of cancer.
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  • Incremental capacity analysis and differential voltage analysis based state of charge and capacity estimation for lithium-ion batteries

    Linfeng Zheng   Jianguo Zhu   Dylan Dah-Chuan Lu   Guoxiu Wang   Tingting He  

    Abstract The reliability and safety of battery operations necessitate an efficient battery management system (BMS) with accurate battery state of charge (SOC) and capacity estimation techniques. This paper investigates the incremental capacity analysis (ICA) and differential voltage analysis (DVA) methods for onboard battery SOC and capacity estimation. Since the conventional cell terminal voltage based ICA/DVA methods are sensitive to the changed battery resistance and polarization during battery aging processes, the SOC based ICA/DVA methods are proposed to address this problem as so to accurately identify features of interest on incremental capacity (IC) and differential voltage (DV) curves for applications. Three feature points (FPs) that are potential to be easily identified by battery management systems are extracted from the SOC based IC/DV curves, and then the relations between FPs and cell SOCs/capacities are quantified and applied for battery SOC and capacity estimation. The robustness of the proposed approach against various aging levels and erroneous cumulative capacities is evaluated. Promising results with the maximum absolute error of 1.0% and the relative error of 2.0% can be achieved for battery SOC and capacity estimation, respectively. Highlights • ICA and DVA methods are developed for onboard battery SOC and capacity estimation. • The SOC based IC/DV curves can reflect the relation between IC/DV values and SOCs. • The relations between feature points and SOCs/capacities are quantified. • The proposed method can perform well even with biased cumulative capacities.
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  • <em>In vitro</em> influence of selenium on the proliferation of and steroidogenesis in goat luteinized granulosa cells

    Xiaolei Yao   M.A. EI-Samahy   Lijie Fan   Linfeng Zheng   Yuyue Jin   Jing pang   Guomin Zhang   Zifei Liu   Feng Wang  

    Abstract In this study, we investigated the effects of Selenium (Se) on the proliferation of and steroidogenesis in goat luteinized granulosa cells (LGCs) and elucidated the mechanisms underlying these effects. Our results showed that proliferating cell nuclear antigen (PCNA), Akt, and phosphoinositide 3-kinase (PI3K) were expressed mainly in ovarian oocytes and granulosa cells (GCs). We observed that 5 ng/mL Se significantly stimulated LGC proliferation, which could be attributed to increases in PCNA, cyclin-dependent kinase 1 (CDK1), phosphorylated adenosine monophosphate-activated protein kinase (p-AMPK; Thr172), and phosphorylated Akt (p-Akt; Ser473) and decreases in p21 ( P  < 0.05). Se treatment also significantly increased estradiol (E 2 ) production, which could be, at least partially, due to increased levels of 3β-hydroxysteroid dehydrogenase( 3β-HSD ), steroidogenic acute regulatory protein ( StAR ), p-Akt (Ser473), and cyclic adenosine monophosphate (cAMP) ( P  < 0.05); however, follicle-stimulating hormone (FSH) significantly enhanced the production of E 2 , progesterone (P 4 ) and cAMP ( P  < 0.05). Moreover, Se treatment stimulated proliferation and the synthesis of E 2 and cAMP in the presence of FSH ( P  < 0.05). Additionally, the expression of antioxidant-related genes [glutathione peroxidase ( GSH-Px ) and superoxide dismutase 2 ( SOD2 )] and the activity of GSH-Px and SOD were progressively elevated by Se treatment ( P  < 0.05). These data suggested that Se plays an important role in the proliferation of and steroidogenesis in LGC by activating the PI3K/Akt and AMPK pathways, thereby increasing the expression of its downstream cell-cycle- and steroid-synthesis-related genes, as well as regulating cellular oxidative stress. Highlights • Appropriate Se concentrations promote in vitro proliferation of goat LGCs. • Se treatment activates the PI3K/Akt- and AMPK-signaling pathways. • Se treatment enhances LGC steroidogenesis via the PI3K/Akt-signaling pathway. • Se treatment strengthens LGC-related stimulatory effects in the presence of FSH.
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  • Comparison of dynamic contrast-enhanced magnetic resonance imaging with T2-weighted imaging for preoperative staging of early endometrial carcinoma

    Linfeng Zheng   Sujuan Zheng   Xiaochun Yuan   Xifu Wang   Zhuoli Zhang   Guixiang Zhang  

    PurposeThis study aimed to compare dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) with T2-weighted imaging (T2WI) for the preoperative staging of early endometrial carcinoma.MethodsThis retrospective study included 22 subjects with early endometrial carcinoma who underwent 3.0 T MRI examination prior to hysterectomy. DCE-MRI and T2WI were evaluated for the preoperative staging of endometrial carcinoma. The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of DCE-MRI and T2WI were assessed and compared using the revised International Federation of Gynecology and Obstetrics surgical staging guidelines (2009) as the reference standard.ResultsOut of the 22 cases of endometrial carcinoma, the use of the reference standard method led to the staging of 14 as IA and eight as IB. The sensitivity, specificity, PPV, NPV, and accuracy of DCE-MRI for preoperative staging were 100% (95% confidence interval: 0.73–1.0), 62.5% (95% CI: 0.26–0.90), 82.4% (95% CI: 0.56–0.95), 100% (95% CI: 0.46–1.0), and 86.4%, respectively, and these values were 85.7% (95% CI: 0.56–0.97), 75% (95% CI: 0.36–0.96), 85.7% (95% CI: 0.56–0.97), 75% (95% CI: 0.36–0.96), and 81.8%, respectively, for T2WI. Thus, the sensitivity and accuracy of DCE-MRI were greater than those of T2WI for preoperative endometrial carcinoma staging.ConclusionDCE-MRI was more sensitive but less specific than T2WI for the preoperative staging of early endometrial carcinoma. DCE-MRI may serve as a useful and reliable tool for the preoperative assessment of endometrial carcinoma.
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  • Breast liposarcoma with solitary metastasis to the pleura

    Lei Lei   Zhanhong Chen   Zhuo Wang   Linfeng Zheng   Yabing Zheng   Xiaojia Wang   Xian Wang  

    AbstractRationale:Breast cancer is the most prevalent malignancy in women worldwide. Our patient presented with a history of breast liposarcoma (LPS) and was found to have pleural metastasis during the initial workup.Patient concerns:The patient was complaining about chest pain and dyspnea that had persisted for a week.Diagnoses:After a full evaluation and histological diagnosis, she was diagnosed as metastatic breast LPS.Interventions:We adopted 6 cycles of pegylated liposomal doxorubicin (PLD) plus ifosfamide as 1st-line palliative chemotherapy, combined with local pleural effusion management.Outcomes:The patient's symptoms were notably relieved, and both malignant metastatic lesions and pleural effusion were controlled.Lessons:Although metastatic breast LPS is rarely reported and incurable, more clinical experience and use of next-generation sequencing should be helpful in finding the effective treatment for metastatic LPS.
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