A stem cell filtering screening enrichment and quickly compounding device, comprises a filter (1), a dual three-way board (2), an elongate pipe (3), a blood circulation driver (4) and a piece of porous material (5). The filter (1) includes a house (11) and a filtering box (12). The house (11) includes a shell (111) and a back cover (112) detachably connected with each other. The shell (111) is in a shape of a cylinder with a front bottom wall while the back cover (112) is in a shape of a cylinder with a back bottom wall, and each bottom wall individually has a hole (1110,1120) in its center; the filtering box (12), in a cylindrical shape, has a strainer-shaped box cover (121) and a strainer-shaped box bottom (122), and the inner chamber of the filtering box (12) is a volume-adjustable stem cell screening and enrichment chamber, furthermore the filtering box (12) is inserted into the back of the shell's chamber. The porous material (5) is disposed in the stem cell screening and enrichment chamber of the filtering box (12), and is matched in shape with the stem cell screening and enrichment chamber. Therefore, the compounding device enables acquiring marrow stem cells, which are highly enriched and compounded on material, during surgery without in vitro cell culture.

An image rotation method and an image rotation system for a video player are provided. The image rotation method includes the following steps. Firstly, an image rotation request from a terminal is received. Then, a source video file is decoded and a rotation processing operation is performed on the decoded source video file in response to the image rotation request, so that a rotated image of the source file is displayed. By the image rotation method and the image rotation system of the present invention, the image in the video player can be rotated by a specified angle. Consequently, the problems of the conventional video player will be overcome. Moreover, since the problems of causing the compressed and distorted image are eliminated, the quality of the played and displayed image is enhanced.

Pure In2O3 is considered as an efficient methanol steam reforming catalyst. Despite of several studies in the past decades, the mechanism of MSR on In2O3 is still not fully understood. In this work, a periodic density functional theory study of the initial dissociation of methanol and water over the In2O3 (110) surface is presented. The activation energy barriers and thermochemistry for several elementary steps are reported. It is found that the energy barriers for O?H bond cleavage of both CH3OH and H2O to produce CH3O and OH species at a surface In-O pair site are very low, indicating that In2O3 (110) can facilely catalyze these two important processes at low temperatures. In addition, the subsequent dehydrogenation of CH3O to CH2O is also found to proceed with a low barrier.

BACKGROUND: Because of a lack of markers for predicting prognosis and an underlying mechanism, patients with primary brainstem hemorrhage (PBH) are currently treated with multiple strategies, but most of them have poor outcomes in a comparison with patients with supratentorial intracranial hemorrhage. Recently, it has been reported that the neutrophil-to-lymphocyte ratio (NLR) represents a novel composite inflammatory marker to predict the prognosis of patients with intracranial hemorrhage, a majority of whom have supratentorial hemorrhage. In this report, we aim to assess the potential predictive value of NLR in patients with PBH. In addition, other available laboratory parameters, including platelet-to-lymphocyte ratio (PLR), and admission blood glucose level (ABG), will be also investigated as markers for prognosis in patients with PBH. METHODS: This study retrospectively enrolled 225 patients with acute PBH who were admitted West China Hospital from January 2012 to December 2016. ABG and absolute numbers of neutrophils, lymphocytes, white blood cells, and platelets were extracted from electronic medical records. ABG, NLR, and PLR were calculated and further assessed using multivariable logistic regression analysis for understanding the associations of treatment outcomes. The comparison of predictive power of independent predictors was evaluated using receiver operating characteristic. RESULTS: Of 225 inpatients, NLR (odds ratio [OR], 1.82; 95% confidence interval [CI]. 1.24 2.62, P< 0.01), PLR (OR, 1.43; 95% CI, 1.11 2.36; P =3D 0.013), and ABG (OR, 6.57; 95% CI, 2.78 15.52; P < 0.01) were independently associated with 90-day status in 112 patients with unfavorable outcomes. All 3 parameters also correlated with admission Glasgow Coma Scale score (r =3D -0.244, P < 0.001; r =3D -0.292, P < 0.001; r =3D -0.661, P < 0.01) and absolute neutrophil counts (r =3D 0.645, P < 0.001; r =3D 0.347, P < 0.001; r =3D 0.695, P < 0.01). Meanwhile, NLR exhibits a comparable predictive power by comparing with PLR (area under the curve [AUC], 0.694; 95%, CI, 0.626-0.764; P < 0.001; versus AUC, 0.662; 95% CI, 0.596-0.724; P < 0.001). In addition, ABG shows a positive predictive value (AUC, 0.784; 95% CI, 0.725 0.832; P < 0.001). The best independent predictive cutoff points were 6.65, 59.3, and 7.81 mmol/L for NLR, PLR, and ABG, respectively. Nevertheless, a combination of 3 parameters shows the best predictive ability (AUC, 0.835; 95% CI, 0.781-0.883; P < 0.001). CONCLUSIONS: NLR, PLR, and ABG can be used to independently predict 90-day functional outcome in patients after PBH. When combined, they have better predictive power in identifying PBH patients with a poor prognosis. To our knowledge, this study is the first to reveal the associations between NLR, PLR, and hyperglycemia and the functional outcomes of patient with PBH. In associating with previously studies on hemorrhage site, our results provide a good opportunity to elucidate the underlying mechanisms of PBH.

BackgroundThe chloroplast-localized ribulose-1, 5-biphosphate carboxylase/oxygenase (Rubisco), the primary enzyme responsible for autotrophy, is instrumental in the continual adaptation of plants to variations in the concentrations of CO2. The large subunit (LSU) of Rubisco is encoded by the chloroplast rbcL gene. Although adaptive processes have been previously identified at this gene, characterizing the relationships between the mutational dynamics at the protein level may yield clues on the biological meaning of such adaptive processes. The role of such coevolutionary dynamics in the continual fine-tuning of RbcL remains obscure.ResultsWe used the timescale and phylogenetic analyses to investigate and search for processes of adaptive evolution in rbcL gene in three gymnosperm families, namely Podocarpaceae, Taxaceae and Cephalotaxaceae. To understand the relationships between regions identified as having evolved under adaptive evolution, we performed coevolutionary analyses using the software CAPS. Importantly, adaptive processes were identified at amino acid sites located on the contact regions among the Rubisco subunits and on the interface between Rubisco and its activase. Adaptive amino acid replacements at these regions may have optimized the holoenzyme activity. This hypothesis was pinpointed by evidence originated from our analysis of coevolution that supported the correlated evolution between Rubisco and its activase. Interestingly, the correlated adaptive processes between both these proteins have paralleled the geological variation history of the concentration of atmospheric CO2.ConclusionsThe gene rbcL has experienced bursts of adaptations in response to the changing concentration of CO2 in the atmosphere. These adaptations have emerged as a result of a continuous dynamic of mutations, many of which may have involved innovation of functional Rubisco features. Analysis of the protein structure and the functional implications of such mutations put forward the conclusion that this evolutionary scenario has been possible through a complex interplay between adaptive mutations, often structurally destabilizing, and compensatory mutations. Our results unearth patterns of evolution that have likely optimized the Rubisco activity and uncover mutational dynamics useful in the molecular engineering of enzymatic activities.ReviewersThis article was reviewed by Prof. Christian Blouin (nominated by Dr W Ford Doolittle), Dr Endre Barta (nominated by Dr Sandor Pongor), and Dr Nicolas Galtier.

In this paper, the model of ink drop and color distortion is first proposed. At first, the ink drop fixing on three-dimensional and status change are analyzed and modeled. Based on this model, Author investigate the reason of color distortions in 3D printing, the parameters is obtained. Then, The range of printing angle and height of 3D model is developed. Finally, under the guidance of the model, the experiments prove that the algorithm is effective in three-dimensional printing.