Yan, Li
Zhan, Cheng
Wang, Shengzi
Wang, Shuyi
Guo, Luo
The aim of this study was to investigate the differences in the gene expression profiles of radiation-sensitive (RS) and radiation-resistant (RR) sinonasal squamous cell carcinoma (SNSCC) and to identify prognostic markers for the radiation reaction of SNSCC. We first examined the differentially expressed genes (DEGs) in RS and RR SNSCC tissues by analyzing clinical samples with GeneChip Human Transcriptome Array 2.0 (HTA 2.0).To understand the functional significance of the molecular changes, we examined the DEGs with Gene Ontology (GO) and pathway analyses to identify the core genes. The expression of several core genes (CCND2, COL5A2, GADD45B, and THBS2) was confirmed with reverse transcription quantitative PCR (RT-qPCR) in a larger series of tissues. We identified 208 DEGs, of which 76 were upregulated and 132 downregulated in the RS tissues relative to the RR tissues. The DEGs were mainly involved in the regulation of cell proliferation, the NF-kappaB signaling pathway, the cell adhesion molecule signaling pathway, and the extracellular matrix-receptor interaction signaling pathway. RT-qPCR confirmed that the CCND2, COL5A2, GADD45B, and THBS2 genes were significantly differentially expressed in the RS and RR tissues, consistent with the GeneChip data. These results extend our understanding of the molecular mechanisms underlying the sensitivity of SNSCC to radiation. The DEGs are involved in the differential response to radiation therapy and the dysregulated core genes identified in this study can be used to predict radiation sensitivity in SNSCC.
Cheng, Xiuyong
Li, Tongchuan
Wang, Honglian
Zhu, Dengna
Ma, Caiyun
Ma, Bingxiang
Wang, Yinghong
Zhang, Junyu
Guo, Luo
Wang, Lei
Yun, Liu
Chen, Shiting
Jiang, Zedong
He, Lin
Genetic polymorphisms of methylenetetrahydrofolate reductase (MTHFR) have been suggested as being associated with cerebral palsy (CP) but the evidence is uncertain. The purpose of this study was to investigate whether MTHFR gene polymorphisms contribute to the development of CP in Chinese infants. For this study, 169 health controls and 159 infants with CP including 43 cases also suffering from mental retardation (MR) were recruited. Genomic DNA was prepared from venous blood and all five single nucleotide polymorphisms in MTHFR (rs4846049, rs1476413, rs1801131, rs1801133 and rs9651118) were genotyped using TaqMan technology. There were no significant differences in allele or genotype frequencies between the CP patients and controls at any of the five genetic polymorphisms. Subgroup analysis found statistically significant difference in allele and genotype frequencies between cases with both CP and MR (CP + MR) compared with both CP-only cases and controls at rs4846049, rs1476413 and rs1801131. The frequencies of the T alleles of rs4846049, rs1476413 and the G allele of rs1801131 were greater in the CP + MR patients than in the CP-only patients and controls. This study provides the first evidence pointing to a MTHFR gene polymorphism as a potential risk factor for CP combined with MR. Journal of Human Genetics (2011) 56, 17-21; doi:10.1038/jhg.2010.127; published online 21 October 2010
Keyue Hu;School of journalism and communication , Beijing Jiaotong University;
With the spread of the epidemic, people's entertainment consumption habits have changed. The TV variety show has become one of the important ways of cultural entertainment for people during the traditional festivals. Among them, the song and dance programs represented by "Tang Palace Banquet", "Luo Shen Shui Fu" and "Guo Se Tian Xiang" have gained in great popularity, attracting the attention of generation Z group(hereinafter referred to as "Gen Z"). This paper focuses on the gratifications of henan TV's songs and dances to "Gen Z" 's aesthetic needs,cognitive needs, social needs and psychological and emotional needs, and studies the Use and Gratifications of "Gen Z" in the song and dance works of TV stations.
CYP3A4 metabolizes more than 50% of the drugs on the market. The large inter-individual differences of CYP3A4 expression may contribute to the variability of human drug responses. Post-transcriptional regulation of CYP3A4 is poorly understood, whereas transcriptional regulation has been studied much more thoroughly. In this study, we used multiple software programs to predict miRNAs that might bind to CYP3A4 and identified 112 potentially functional miRNAs. Then a luciferase reporter system was used to assess the effect of the overexpression of each potentially functional miRNA in HEK 293T cells. Fourteen miRNAs that significantly decreased reporter activity were measured in human liver samples (N =3D 27) as candidate miRNAs. To establish a more effective way to analyze in vivo data for miRNA candidates, the relationship between functional miRNA and target mRNA was modeled mathematically. Taking advantage of this model, we found that hsa-miR-577, hsa-miR-1, hsa-miR-532-3p and hsa-miR-627 could significantly downregulate the translation efficiency of CYP3A4 mRNA in liver. This study used in silico, in vitro and in vivo methods to progressively screen functional miRNAs for CYP3A4 and to enhance our understanding of molecular events underlying the large inter-individual differences of CYP3A4 expression in human populations. =20
Sang, Qing
Mei, Honglin
Kuermanhan, Ahan
Feng, Ruizhi
Guo, Luo
Qu, Ronggui
Xu, Yao
Li, Huawei
Jin, Li
He, Lin
Wang, Lei
With a prevalence of 0.1 %, hearing loss is among the most common sensory impairments and affects several million people around the world. Identification of deafness-related genes or loci may facilitate basic research and clinical translational research of the disorder. The PTPRQ gene encodes protein tyrosine phosphatase receptor Q, which is required for the formation of shaft connectors and the normal maturation and development of hair bundles in the mammalian cochlea. Here, we present the genetic and molecular characteristics of a Kazakh family with an autosomal recessive non-syndromic hearing impairment, DFNB84. Using whole-exome sequencing, we identified two mutations that together form a novel compound heterozygous mutation in PTPRQ. Sanger sequencing confirmed that the affected members inherited both the c.16_17insT (L8fsX18) and c.2714delA (E909fsX922) mutations. Both mutations lead to a frameshift and a truncated form of the protein. The novel compound heterozygous mutation co-segregated with hearing loss in this family, and neither of the two mutations was found in 200 healthy Kazakh controls or in any of the public databases. In the study, we identified novel mutations in PTPRQ responsible for DFNB84. This is the third report of PTPRQ mutations involved in deafness and the first report of familial deafness in China. The identification of novel mutations in PTPRQ presented here further confirms the essential role of PTPRQ in hearing development and auditory function. Our data provide additional molecular information for establishing a better genotype-phenotype understanding of DFNB84.=20
Guo, Luo
Du, Shihong
Haining, Robert
Zhang, Lianjun
The existing indicators related to spatial association, especially the K function, can measure only the same dimension of vector data, such as points, lines and polygons, respectively. We develop four new indicators that can analyze and model spatial association for the mixture of different dimensions of vector data, such as lines and points, points and polygons, lines and polygons. The four indicators can measure the spatial association between points and polygons from both global and local perspectives. We also apply the presented methods to investigate the association of temples and villages on land-use change at multiple distance scales in the Guoluo Tibetan Autonomous Prefecture in Qinghai Province, PR China. Global indicators show that temples are positively associated with land-use change at large spatial distances (e.g., >6000 m), while the association between villages and land-use change is insignificant at all distance scales. Thus temples, as religious and cultural centers, have a stronger association with land-use change than the places where people live. However, local indicators show that these associations vary significantly in different sub-areas of the study region. Furthermore, the association of temples with land-use change is also dependent on the specific type of land-use change. The case study demonstrates that the presented indicators are powerful tools for analyzing the spatial association between points and polygons. (C) 2011 Elsevier B.V. All rights reserved.