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Interferon gamma up-regulates alpha-2 macroglobulin expression in human astrocytoma cells

Journal:
Journal of Neuroimmunology


Abstract(summary):

An established human astrocytoma cell line (T67) was shown to constitutively produce the proteinase inhibitor alpha-2macroglobulin (alpha-2M). Interferon gamma (IFN-gamma), a potent immunoregulatory lymphokine, was able to increase the synthesis of alpha-2M by these cells, as measured by ELISA on cell supernatants. The alpha-2M induction was also observed in other human glioma cell lines (T70 and ADF) and in human fetal astrocyte cultures following IFN-gamma treatment. In T67 cells this effect was dose-dependent and the maximum (2.7-fold increase) was obtained with 2000 U/ml of IFN-gamma. A corresponding enhanced alpha-2M mRNA accumulation was demonstrated by PCR and Northern blot techniques. Our results suggest an important role of alpha-2M during inflammatory and immune processes in the CNS. An increased release of alpha-2M following IFN-gamma stimulation may allow the removal of the bulk of proteases released at the site of inflammation, strengthening at the same time the antigen presentation processes.


Page:
31---37


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