A ratiometric fluorescence probe for monitoring cyanide ion in live cells

Author:

Chao, Jianbin  Li, Zhiqing  Zhang, Yongbin  Huo, Fangjun  Yin, Caixia  Tong, Hongbo  Liu, Yuhong  

Journal:

SENSORS AND ACTUATORS B-CHEMICAL

Issue Date:

2016

Abstract(summary):

A ratiometric emission fluorescent probe 1,1-dimethy1-3-ethyl 2 (2 (N ethyl carbazol-3-y1)-viny1)-1Hbenzo[e]indolium iodide (Probe) is facilely synthesized via the ethylene bridging of N-ethylcarbazole3-formaldehyde and 1,1,2-trimethy1-3-ethyl-1H-benz[e]indolium iodide, which is a useful fluorescent probe for monitoring cyanide ion at extremely low concentrations, quantitatively. The detection of cyanide is performed via the nucleophilic attack of cyanide toward the benzo[e]indolium group of the probe, resulting in a prominent fluorescence ratiometric change and a color change. The titration experiments for CN-show that it exhibits a large Stokes shift (92 nm), high selectivity, excellent sensitivity and good stability for CN-sensing in DMSO system. The detection limit of Probe for CN-is found to be 0.23 M. Furthermore, the probe has excellent cell membrane permeability and is applied successfully to rapidly detect CN-in living cells. (C) 2016 Elsevier B.V. All rights reserved.

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