Fluorescence completely separated ratiometric probe for HClO in lysosomes

Author:

Zhen Zhang  Jiangli Fan  Guanghui Cheng  Sahar Ghazali  Jianjun Du  Xiaojun Peng  

Journal:

Sensors and Actuators B: Chemical

Issue Date:

2017

Abstract(summary):

Graphical abstract Highlights • A ratiometric probe (BFClO) with completely seperated fluorescent signals for sensing HOCl is reported. • BFClO exhibits superior selectivity (>300 fold) and fast response (within 10 s) for HClO over other ROS in a broad pH range. • BFClO can be used for visualizing exogenous and endogenous HOCl in lysosomes of living cells. Abstract Hypochlorous acid (HClO), as one of the reactive oxygen species (ROS), was reported to be involved in a number of human diseases and many efforts have therefore been made to probe its biological roles at molecular level. In this work, a ratiometric probe (BFClO) with completely separated fluorescent signals consisting of a boron-dipyrromethene (BODIPY) dye conjugated with Fisher aldehyde was designed and synthesized for the selective and sensitive detection of HOCl via oxidative cleavage of the alkene linker. The probe evinced more than 300-fold enhancement in fluorescence ratio (I 511nm /I 713nm ) in the presence of HClO over other ROS with fast response time (within 10 s) and low detection limit (10.6 nM). Confocal fluorescence images demonstrated that the probe could well permeate through plasma membrane and visualize exogenous and endogenous HOCl in lysosomes of living cells.

Page:

293-293