Creat membership Creat membership
Sign in

Forgot password?

Confirm
  • Forgot password?
    Sign Up
  • Confirm
    Sign In
Creat membership Creat membership
Sign in

Forgot password?

Confirm
  • Forgot password?
    Sign Up
  • Confirm
    Sign In
Collection
For ¥0.57 per day, unlimited downloads CREATE MEMBERSHIP Download

toTop

If you have any feedback, Please follow the official account to submit feedback.

Turn on your phone and scan

home > search >

Shoot organogenesis from leaf explants ofDayaoshania cotinifoliaW. T. Wang

Author:
Yang, Guo   Lü, Jinfeng   Teixeira da Silva, Jaime A.   Chen, Hongfeng   Ma, Guohua  


Journal:
In Vitro Cellular & Developmental Biology - Plant


Issue Date:
2014


Abstract(summary):

Dayaoshania cotinifolia W. T. Wang is a rare and endangered member of the Gesneriaceae family which is endemic to China. To conserve this species, an efficient in vitro propagation and regeneration system via shoot organogenesis was established from young leaf explants. Adventitious shoot induction was possible within 50-60 d on basal Murashige and Skoog medium supplemented with 1-3 mu M 6-benzyladenine, although 5 mu M 6-benzyladenine induced hyperhydricity. Basal medium containing 1-5 mu M thidiazuron induced fewer shoots, while 1-5 mu M alpha-naphthaleneacetic acid induced numerous adventitious roots and a few adventitious shoots. However, when thidiazuron and alpha-naphthaleneacetic acid were combined, both the induction percentage and number of shoots increased. Leaf explants cultured on induction medium supplemented with 1-5 mu M 2,4-dichlorophenoxyacetic acid become necrotic and died. Induction medium supplemented with 1 mu M alpha-naphthaleneacetic acid and 1-3 mu M 6-benzyladenine was optimal for inducing adventitious shoots as was the combination of 1-3 mu M thidiazuron and 1 mu M alpha-naphthaleneacetic acid. Induction medium containing 2.0 mu M 6-benzyladenine and 0.5 mu M indole-3-acetic acid was optimal for the multiplication of adventitious shoots. Rooting was achieved on half-strength MS medium supplemented with 3.0 mu M indole-3-acetic acid or alpha-naphthaleneacetic acid and 0.1% activated charcoal. Plantlets were transplanted to a mixture of sand, vermiculite, and humus (1:1:1); 92% survived. This protocol is a unique and effective means to micropropagate this rare and important plant and could serve as a solution for in vitro and ex vitro conservation.


Page:
451-457


VIEW PDF

The preview is over

If you wish to continue, please create your membership or download this.

Create Membership

Similar Literature

Submit Feedback

This function is a member function, members do not limit the number of downloads