Quinoline-Based Fluorescent Probe for Ratiometric Detection of Lysosomal pH

Author:

Li, Guoping   Zhu, Dongjian   Xue, Lin   Jiang, Hua  

Journal:

Organic Letters

Issue Date:

2013

Abstract(summary):

A new pH-responsive fluorescent probe has been reported based on protonation-activable resonance charge transfer. In aqueous solution, probe PQ-Lyso exhibits ratiometric detection of pH changes with a large hypsochromic shift of 76 nm and remarkable changes in the fluorescence intensity ratio (R = F-494 (nm)/F-570 (nm), R/R-0 = 105). Furthermore, PQ-Lyso, which is localized to lysosomes in living cells, can calibrate lysosomal pH using fluorescence ratiometry.

Page:

5020-5023