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Differential cytokine gene expression in CD4+ and CD8+ T cell subsets of calves

Author:
Sachi Tanaka   Hisashi Aso   Kohtaro Miyazawa   Yasuhiro Nagai   Kouichi Watanabe   Shyuichi Ohwada   Jin Kobayashi    Takahiro Yamaguchi  


Journal:
Veterinary Immunology and Immunopathology


Issue Date:
2007


Abstract(summary):

The distinct patterns of cytokine expression in CD4+ and CD8+ T cells are well understood in mice and humans. However, little information is available about cytokine expression in bovine CD4+ and CD8+ T cells. In this study, mRNA expression of 19 different cytokines was analyzed in CD4+ and CD8+ T cells of calves with or without Concanavalin A (Con A) stimulation. CD4+ and CD8+ T cell populations were enriched to 98%purity by positive selection using magnetic cell sorting (MACS). CD4+ T cells spontaneously expressed the mRNAs of interleukin-1α (IL-1α), IL-1β, IL-2, IL-6, IL-7, IL-8, IL-10, IL-18, IFN-γ, TNF-α, TNF-β and TGF-β, and augmented the mRNA expression of IL-10, IFN-γ and TNF-β after Con A stimulation. The mRNAs of IL-3, IL-4, IL-5, IL-13 and GM-CSF were newly expressed in Con A-stimulated CD4+ T cells. CD8+ T cells displayed spontaneous mRNA expression of IL-6, IL-18, TNF-α, TNF-β and TGF-β, and newly expressed the mRNA of IL-2, IL-7, interferon-gamma (IFN-γ) and GM-CSF after Con A stimulation. It was found that CD4+ T cells expressed the mRNA of 17 cytokines except for IL-12 and IL-15, while CD8+ T cells expressed only the mRNA of 9 cytokines after Con A stimulation. The profile of cytokine mRNA expression was substantially different in the CD4+ and CD8+ T cells of calves, indicating that CD4+ T cells can be distinguished from CD8+ T cells by the cytokine gene expression of IL-1α, IL-1β, IL-3, IL-4, IL-5, IL-8, IL-10 and IL-13. Differential cytokine expression between CD4+ and CD8+ T cells serve to interpret an individual function of T cell subsets in the immune system of calves.


Page:
84-91


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