The genes for type XIII collagen (COL13A1) and prolyl 4-hydroxylase (P4HA) were previously assigned to human chromosome 10q by radioactivein situhybridization. Here we have applied fluorescencein situhybridization combined with targets representing different levels of resolution to determine, first, the order of these genes along chromosome 10; second, their transcriptional orientation; and third, the distance between these genes. The order along the chromosome was determined to be centromere–COL13A1–P4HA–telomere using mechanically stretched chromosomes. By combining the data from stretched chromosomes and interphase nuclei, we found that the transcriptional orientation were tail to tail (COL13A1 3′–3′ P4HA). The distance between these genes was measured by fiber FISH to be 550 kb.
Page:
299-302
Related
Batch download
Cited By
noting
Similar Literature
Submit Feedback
Please wait while the file you selected is being converted