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Two New Methods for Early Detection of the Effects of Herbicides in Plants Using Biomarkers

Journal:
Journal of Planar Chromatography - Modern TLC


Issue Date:
2009


Abstract(summary):

Two simple and rapid methods for early detection of the effects of herbicides using two different groups of plant biomarkers are presented. The methods are based on High Performance Planar Chromatography (HPPC) and have been developed as field-tests (Herbicide Weed Response test (HWR-Test (R)). Phytochemical changes can be detected before any morphological changes are visible on the plants. These changes are defined as biomarkers and can be detected by HPPC-screening. After overall identification of the phytochemical biomarker pattern by HPPC-screening, two different biomarker groups, carbohydrates and amino acids, were detected using modified HPPC reagents for color reactions. The color reactions of the two biomarker groups four days after exposure were correlated with biomass reduction 21 days after exposure in extracts of unexposed plants and plants exposed to herbicides. The method can, thus, be used vis an early indicator for predicting the extent of the effects of herbicides on plants.


Page:
65---71


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