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Proteomic analysis of human immunodeficency virus using liquid chromatography/tandem mass spectrometry effectively distinguishes specific incorporated host proteins

Author:
Saphire, ACS  Gallay, PA  Bark, SJ  


Journal:
JOURNAL OF PROTEOME RESEARCH


Issue Date:
2006


Abstract(summary):

A major challenge to studying virus-incorporated host proteins is the fact that they are not encoded by the viral genome. We used Liquid Chromatography/Tandem Mass Spectrometry (LC-MS/MS) on whole virions to obtain a snapshot of the HIV-1 proteome. We identified known viral and host-cellular proteins and also identified novel components of HIV-1 and confirm these by traditional biochemical methods. Our comparison of wild-type and mutant viruses demonstrates that LC-MS/MS has the specificity to distinguish the presence/absence of a single host protein in intact virions.


Page:
530---538


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